Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,561	A→G	D56G (GAC→GGC)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,561	0	A	G	100.0%	219.2 / NA	63	D56G (GAC→GGC)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/35);  total (28/35)

GGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTT  >  NC_000913/285425‑285696                                                                                                                                         |                                                                                                                                        ggCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGgcgc                                                                                                                                      <  1:128040/140‑1 (MQ=255) ggCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGgcgc                                                                                                                                      <  1:224292/140‑1 (MQ=255)      cGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCa                                                                                                                                 <  1:539176/140‑1 (MQ=255)          cTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGa                                                                                                                             <  1:453345/140‑1 (MQ=255)            gggAGTCACCGCCTGCGGCTTCGTCTAGCAGGCCACGATGCTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTCTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGAcc                                                                                                                           <  2:922702/140‑1 (MQ=255)             ggAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGAccc                                                                                                                          <  2:416280/140‑1 (MQ=255)              gagaCACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCg                                                                                                                         <  2:317733/140‑1 (MQ=255)                gaCACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCt                                                                                                                       <  1:278473/140‑1 (MQ=255)                   acCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGAt                                                                                                                    >  2:111856/1‑140 (MQ=255)                       ccTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATgggg                                                                                                                >  2:300041/1‑140 (MQ=255)                       ccTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATgggg                                                                                                                >  2:64889/1‑140 (MQ=255)                        cTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGgc                                                                                                               <  1:644747/140‑1 (MQ=255)                        cTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGgc                                                                                                               <  2:749986/140‑1 (MQ=255)                        cTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGgc                                                                                                               >  2:644747/1‑140 (MQ=255)                           cGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGgctgc                                                                                                            >  2:229837/1‑140 (MQ=255)                            ggCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATg                                                                                                                   >  1:65686/1‑132 (MQ=255)                            ggCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATg                                                                                                                   <  2:65686/132‑1 (MQ=255)                              cTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTgg                                                                                                         <  2:737120/140‑1 (MQ=255)                                tCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTa                                                                                                       <  1:111856/140‑1 (MQ=255)                                  gTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGa                                                                                                     <  1:229837/140‑1 (MQ=255)                                           gCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGAccgcacccg                                                                                            >  2:849409/1‑140 (MQ=255)                                           gCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGAccgcacccg                                                                                            >  2:227956/1‑140 (MQ=255)                                             cACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGAccgcacccgca                                                                                          >  1:220536/1‑140 (MQ=255)                                              aCGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGAccgcacccgcac                                                                                         <  2:931108/140‑1 (MQ=255)                                              aCGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGAccgcacccgcac                                                                                         <  1:132567/140‑1 (MQ=255)                                                gATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCAcgc                                                                                       >  2:941459/1‑140 (MQ=255)                                                   gTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGc                                                                                    <  1:849409/140‑1 (MQ=255)                                                   gTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGc                                                                                    <  1:941459/140‑1 (MQ=255)                                                     tCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCAc                                                                                  >  1:132804/1‑140 (MQ=255)                                                     tCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCAc                                                                                  >  2:342398/1‑140 (MQ=255)                                                       ctgctgGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACgg                                                                                >  1:904757/1‑140 (MQ=255)                                                         gctgGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGcc                                                                              <  1:342398/140‑1 (MQ=255)                                                            ggCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGt                                                                           >  1:618435/1‑140 (MQ=255)                                                             gCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGtt                                                                          >  1:935841/1‑140 (MQ=255)                                                              ccTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTc                                                                         >  1:762424/1‑140 (MQ=255)                                                               cTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATTGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGGAGACCGCACCCGCACGCGGCACGGCCCGTTcc                                                                        >  2:252936/1‑140 (MQ=255)                                                                tATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCg                                                                       <  1:227956/140‑1 (MQ=255)                                                                  tttCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGcc                                                                     <  1:64889/140‑1 (MQ=255)                                                                  tttCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGcc                                                                     <  2:935841/140‑1 (MQ=255)                                                                  tttCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGcc                                                                     <  1:449581/140‑1 (MQ=255)                                                                           cGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgc                                                            <  1:351593/140‑1 (MQ=255)                                                                            gACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgct                                                           >  1:936101/1‑140 (MQ=255)                                                                             aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                          >  1:523317/1‑140 (MQ=255)                                                                             aCGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCctgctg                                                          >  2:579375/1‑140 (MQ=255)                                                                                  ttCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTgggg                                                     >  2:374416/1‑140 (MQ=255)                                                                                         tGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATc                                              <  2:904757/140‑1 (MQ=255)                                                                                           tCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATccc                                            >  2:893877/1‑140 (MQ=255)                                                                                           tCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATccc                                            >  1:451191/1‑140 (MQ=255)                                                                                             ggcggGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGt                                          <  1:374416/140‑1 (MQ=255)                                                                                             ggcggGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGt                                          <  1:579375/140‑1 (MQ=255)                                                                                                         gggCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCt                              <  2:451191/140‑1 (MQ=255)                                                                                                         gggCACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCt                              <  2:708751/140‑1 (MQ=255)                                                                                                            cACGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGCCTGCg                           >  2:608366/1‑140 (MQ=255)                                                                                                             aCGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGt                          >  2:105449/1‑140 (MQ=255)                                                                                                              cGCTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTg                         >  1:885586/1‑140 (MQ=255)                                                                                                               gCTGTTTTTGGTCTCCCGCGGGCTCGGCGCCGCCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCCTCCCGTTCGGCATCGTCTGCGTGc                        <  2:618435/140‑1 (MQ=255)                                                                                                                cTGTTTTTGGTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCt                       <  1:893877/140‑1 (MQ=255)                                                                                                                         gTCTCCCGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTa              <  1:300041/140‑1 (MQ=255)                                                                                                                             cccGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACg          >  2:363155/1‑140 (MQ=255)                                                                                                                             cccGCGTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACg          >  2:414938/1‑140 (MQ=255)                                                                                                                                  gTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa     <  2:885586/140‑1 (MQ=255)                                                                                                                                  gTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa     <  1:309024/140‑1 (MQ=255)                                                                                                                                  gTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa     <  1:171962/140‑1 (MQ=255)                                                                                                                                  gTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa     <  2:523317/140‑1 (MQ=255)                                                                                                                                  gTGCTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGACCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGa     <  1:28817/140‑1 (MQ=255)                                                                                                                                     cTCGGCGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGCCATCGCCTCCGTGCTGCCCTTCTACACCCCGGACtt  >  1:196640/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GGCTACGGGCTGGGAGACACCGCCTGCGGCTTCGTCTGGCAGGCCACGATGTTCCTGCTGGCCTATTTCTACACCGACGTCTTCGGCCTGTCGGCGGGGATTATGGGCACGCTGTTTTTGGTCTCCCGCGTGCTCGACGCCGTCACCGACCCGCTGATGGGGCTGCTGGTAGACCGCACCCGCACGCGGCACGGCCAGTTCCGCCCGTTCCTGCTGTGGGGGGCCATCCCGTTCGGCATCGTCTGCGTGCTGACCTTCTACACGCCGGACTT  >  NC_000913/285425‑285696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑