Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,446,348	C→T	T746I (ACC→ATC)	tamB →	translocation and assembly module subunit TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,446,348	0	C	T	100.0%	229.2 / NA	65	T746I (ACC→ATC)	tamB	translocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/35);  total (30/35)

TCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCT  >  NC_000913/4446213‑4446483                                                                                                                                        |                                                                                                                                        tCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACt                                                                                                                                     >  2:396772/1‑140 (MQ=255)    ggCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAg                                                                                                                                  >  2:460736/1‑140 (MQ=255)     gCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAgg                                                                                                                                 <  1:251655/140‑1 (MQ=255)     gCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAgg                                                                                                                                 <  2:612480/140‑1 (MQ=255)      ccACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGc                                                                                                                                <  1:943507/140‑1 (MQ=255)      ccACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGc                                                                                                                                <  1:956994/140‑1 (MQ=255)         cacTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGc                                                                                                                                <  1:648070/137‑1 (MQ=255)         cacTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGc                                                                                                                                >  2:648070/1‑137 (MQ=255)             gctgGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGt                                                                                                                         <  1:460736/140‑1 (MQ=255)              ctgGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTa                                                                                                                        >  2:45031/1‑140 (MQ=255)                  cTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCtt                                                                                                                    <  1:878226/140‑1 (MQ=255)                   ttAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTc                                                                                                                   >  2:122597/1‑140 (MQ=255)                      aCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAgg                                                                                                                                 >  1:242606/1‑123 (MQ=255)                      aCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAgg                                                                                                                                 <  2:242606/123‑1 (MQ=255)                      aCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACg                                                                                                                >  1:511316/1‑140 (MQ=255)                      aCCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACg                                                                                                                >  1:172986/1‑140 (MQ=255)                        ccGAATGCGGATCTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACggg                                                                                                              <  1:263025/140‑1 (MQ=255)                                 gAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGa                                                                                                     >  2:445772/1‑140 (MQ=255)                                 gAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGa                                                                                                     >  1:235565/1‑140 (MQ=255)                                 gAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGa                                                                                                     <  2:805259/140‑1 (MQ=255)                                   aCTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACg                                                                                                   <  2:402980/140‑1 (MQ=255)                                     tgtgCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGtt                                                                                                 >  2:593282/1‑140 (MQ=255)                                       tgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGc                                                                                               >  2:619453/1‑140 (MQ=255)                                       tgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGc                                                                                               >  2:648665/1‑140 (MQ=255)                                       tgcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGc                                                                                               >  1:152627/1‑140 (MQ=255)                                        gcgtgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGcc                                                                                              <  2:235565/140‑1 (MQ=255)                                           tgcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTgg                                                                                           <  2:246147/140‑1 (MQ=255)                                            gcCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTggg                                                                                          >  1:797214/1‑140 (MQ=255)                                             ccGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGa                                                                                         <  1:122597/140‑1 (MQ=255)                                             ccGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGa                                                                                         <  2:743850/140‑1 (MQ=255)                                                  aaCTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGAcacca                                                                                    <  2:386181/140‑1 (MQ=255)                                                              gggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGc                                                                        <  2:152627/140‑1 (MQ=255)                                                              gggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGc                                                                        <  2:126562/140‑1 (MQ=255)                                                               ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGGCCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCt                                                                       >  2:628326/1‑140 (MQ=255)                                                               ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCt                                                                       >  2:319951/1‑140 (MQ=255)                                                               ggggCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCt                                                                       >  2:1161/1‑140 (MQ=255)                                                                   ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGGCCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg                                                                   <  1:628326/140‑1 (MQ=255)                                                                   ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg                                                                   <  1:493744/140‑1 (MQ=255)                                                                   ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg                                                                   <  1:445772/140‑1 (MQ=255)                                                                   ccGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCg                                                                   <  1:648665/140‑1 (MQ=255)                                                                      aaGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAg                                                                >  2:175141/1‑140 (MQ=255)                                                                      aaGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCACAg                                                                >  2:860594/1‑140 (MQ=255)                                                                              gCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTAt                                                        <  1:687539/140‑1 (MQ=255)                                                                                 gtggtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCAc                                                     >  2:336129/1‑140 (MQ=255)                                                                                 gtggtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCAc                                                     <  1:619453/140‑1 (MQ=255)                                                                                   ggtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCAccc                                                   >  1:823395/1‑140 (MQ=255)                                                                                   ggtgAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCAccc                                                   <  1:336129/140‑1 (MQ=255)                                                                                            cAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGc                                          >  1:369933/1‑140 (MQ=255)                                                                                            cAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGc                                          >  1:290038/1‑140 (MQ=255)                                                                                             aaCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCg                                         <  2:511316/140‑1 (MQ=255)                                                                                              aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                        <  1:860594/140‑1 (MQ=255)                                                                                              aCCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGt                                        <  1:175141/140‑1 (MQ=255)                                                                                                 gCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAAc                                     >  1:51065/1‑140 (MQ=255)                                                                                                    tCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGt                                   <  2:913341/139‑1 (MQ=255)                                                                                                    tCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGt                                   >  1:913341/1‑139 (MQ=255)                                                                                                     cGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGACAGTATCACCCTTTc                                               <  2:547311/126‑1 (MQ=255)                                                                                                     cGACCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGACAGTATCACCCTTTc                                               >  1:547311/1‑126 (MQ=255)                                                                                                       aCCTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAg                               <  1:959056/140‑1 (MQ=255)                                                                                                        ccTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAgg                              <  1:319951/140‑1 (MQ=255)                                                                                                         cTCGCCATGCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGt                             <  1:45031/140‑1 (MQ=255)                                                                                                                 gCTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCaaa                     <  1:636215/140‑1 (MQ=255)                                                                                                                  cTGAAACCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAAc                    >  2:251791/1‑140 (MQ=255)                                                                                                                     aaaCCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGt                                   >  1:321459/1‑122 (MQ=255)                                                                                                                     aaaCCGTTTATGCCAGAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGt                                   <  2:321459/122‑1 (MQ=255)                                                                                                                                    gAAATCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCt  >  2:750337/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGGGCCACACTGCTGGCTTAACCCGAATGCGGAACTGTGCGTGCCGCAAACTATCGATGCGGGGGCCGAAGGGCGTGCGGTGGTGAATCTCAACCGCTTCGACCTCGCCATGCTGAAACCGTTTATGCCAGAAACCACTCAGGCCAGCGGTATCTTCACGGGTAAAGCGGACGTTGCCTGGGACACCACGAAAGAGGGGCTGCCGCAGGGCAGTATCACCCTTTCGGGGCGTAACGTGCAGGTAACGCAAACCGTCAACGATGCGGCGCT  >  NC_000913/4446213‑4446483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑