Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,536,171	G→A	intergenic (+140/+443)	yjhR → / ← nanS	KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR/N‑acetyl‑9‑O‑acetylneuraminate esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,536,171	0	G	A	100.0%	145.1 / NA	42	intergenic (+140/+443)	yjhR/nanS	KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR/N‑acetyl‑9‑O‑acetylneuraminate esterase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/27);  total (15/27)

GTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGC  >  NC_000913/4536034‑4536305                                                                                                                                          |                                                                                                                                       gtgATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCAcc                                                                                                                                      <  2:740549/140‑1 (MQ=255)   gatgaGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTg                                                                                                                                    >  2:24589/1‑140 (MQ=255)            ttCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATg                                                                                                                           >  2:691572/1‑140 (MQ=255)                tGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCgg                                                                                                                       <  2:807433/140‑1 (MQ=255)                tGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCgg                                                                                                                       <  2:657008/140‑1 (MQ=255)                tGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCgg                                                                                                                       <  1:337263/140‑1 (MQ=255)                  aTACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGt                                                                                                                     <  2:205039/140‑1 (MQ=255)                   tACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCgg                                                                                                                       <  1:524463/137‑1 (MQ=255)                   tACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCgg                                                                                                                       >  2:524463/1‑137 (MQ=255)                      aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCtt                                                                                                                 >  1:235209/1‑140 (MQ=255)                      aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCtt                                                                                                                 <  2:463698/140‑1 (MQ=255)                      aGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGCtt                                                                                                                 >  1:1001163/1‑140 (MQ=255)                             gtaAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGaa                                                                                                          >  1:997735/1‑140 (MQ=255)                                  gggCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGGTTTTTTGAATtaaa                                                                                                     >  2:229816/1‑140 (MQ=255)                                       tatTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGCTTTTTTGAATGaaaaaaaa                                                                                                >  1:355080/1‑140 (MQ=255)                                           ttACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgca                                                                                            <  1:877490/140‑1 (MQ=255)                                              ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagc                                                                                         <  2:615959/140‑1 (MQ=255)                                              ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagc                                                                                         <  1:691572/140‑1 (MQ=255)                                              ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagc                                                                                         <  2:997735/140‑1 (MQ=255)                                              ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGGTTTTTTGGATGAAAGaaaaaacaaaa                                                                                         >  1:708380/1‑133 (MQ=255)                                              ccAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGGTTTTTTGAATGAAAGaaaaaaaaaga                                                                                         >  2:821613/1‑133 (MQ=255)                                                aaTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                       <  2:277235/140‑1 (MQ=255)                                                aaTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                       <  1:996164/140‑1 (MQ=255)                                                aaTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                       <  1:423090/140‑1 (MQ=255)                                                aaTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaagcaa                                                                                       <  1:387183/140‑1 (MQ=255)                                                aaTGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTCGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATgcaaacaa                                                                                       <  2:722600/140‑1 (MQ=255)                                                            aCTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGGTTTTTTGAATGAAAGAAAAAGCAAACAATAAAAtaataaa                                                                           >  1:895438/1‑139 (MQ=255)                                                             cTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGCTGTTAGGATTTACAGAAAAGACAAAAACtaatataatatag                                                                          >  2:441199/1‑137 (MQ=255)                                                                        aTTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGCTTTTTGGAATGAAAAAAAAAACAAACAAAAATATAATAAAAGCCTAAttttt                                                               >  1:119258/1‑138 (MQ=255)                                                                             gAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGaa                                                          <  1:679732/140‑1 (MQ=255)                                                                             gAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGaa                                                          <  1:1013141/140‑1 (MQ=255)                                                                             gAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGaa                                                          <  1:229816/140‑1 (MQ=255)                                                                             gAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGaa                                                          <  1:451187/140‑1 (MQ=255)                                                                              aGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAAc                                                         <  1:178086/140‑1 (MQ=255)                                                                                    cTTTCCTGATTATAAATCTTATTGTATACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCAtt                                                   <  2:690847/140‑1 (MQ=255)                                                                                     tttCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTa                                                  <  2:284682/140‑1 (MQ=255)                                                                                              tataAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGGTTTTTTGGATGAAAAAAAAAGAAAAGAAAAAAATAAAAAATGACTAATTTTTTTTAAAAACATTAAACaaatta                                         >  2:702494/1‑138 (MQ=255)                                                                                                                 cTAATCATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGa                      <  1:155596/140‑1 (MQ=255)                                                                                                                      cATTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGGGGTTTTTTGAATGAAAGAAAAAACAAGCAAAAAAATAAAAAAAAACTAATTTTTTTTTAAATTATTAAAAATATAATAATCGTCTATTTTTCAGAAtacc                 >  2:80228/1‑137 (MQ=255)                                                                                                                       gacagccTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGt                <  1:121931/135‑1 (MQ=255)                                                                                                                       aTTTTCCTTTGCATAATGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTAATTGCCAGAATCCGt                <  1:1043296/140‑1 (MQ=255)                                                                                                                                     aaTGCACCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGc  <  1:597152/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       GTGATGAGCCGTTCATGATACAGGAGTAGTAAAGGGCATATTTTACCAATGTTAAGGCGACTTTCCGTTTTATTGCGAGTCGCCTTTCCTGATTATAAATCTTATTGTTTACCTAATCATTTTCCTTTGCATAATGCGCCTGGGATTAATGGCGGGTGCTTTTAGGAATGAAAGAAAATGCAAGCAATAATATAATAATGGCCTAATTTCTTTGAACATCATTAAGCATATCATCATCGTCTATTTGCCAGAATCCGTCGACTTATAATCGC  >  NC_000913/4536034‑4536305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑