Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,552,826	A→G	intergenic (+6/‑75)	uxuA → / → uxuB	D‑mannonate dehydratase/D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,552,826	0	A	G	100.0%	207.8 / NA	57	intergenic (+6/‑75)	uxuA/uxuB	D‑mannonate dehydratase/D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

CGGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATT  >  NC_000913/4552691‑4552955                                                                                                                                        |                                                                                                                                  cGGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCgg                                                                                                                               <  1:20887/140‑1 (MQ=255)  ggTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGc                                                                                                                              >  1:777815/1‑140 (MQ=255)             gCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTgcgcg                                                                                                                   <  1:183919/140‑1 (MQ=255)             gCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTgcgcg                                                                                                                   >  2:310934/1‑140 (MQ=255)                ggACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCg                                                                                                                >  2:810123/1‑140 (MQ=255)                ggACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCg                                                                                                                >  1:436507/1‑140 (MQ=255)                     acCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCg                                                                                                           >  2:714231/1‑140 (MQ=255)                      ccTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTgcgcgc                                                                                                                  <  1:435528/132‑1 (MQ=255)                      ccTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTgcgcgc                                                                                                                  >  2:435528/1‑132 (MQ=255)                                  aaccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCt                                                                                              <  2:518082/140‑1 (MQ=255)                                    ccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTg                                                                                            >  1:219298/1‑140 (MQ=255)                                    ccaaccCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTg                                                                                            >  1:161999/1‑140 (MQ=255)                                       accCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCg                                                                                         >  1:200803/1‑140 (MQ=255)                                       accCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCg                                                                                         >  1:64779/1‑140 (MQ=255)                                         ccAGGTTACTCCGCGATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTc                                                                                       <  2:1000479/140‑1 (MQ=255)                                            ggTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCAct                                                                                    >  2:609725/1‑140 (MQ=255)                                               tACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCGTGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCtt                                                                                 >  2:1013787/1‑140 (MQ=255)                                                 cTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGGACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTg                                                                               >  1:438981/1‑140 (MQ=255)                                                 cTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTg                                                                               <  1:494415/140‑1 (MQ=255)                                                 cTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTg                                                                               >  2:194881/1‑140 (MQ=255)                                                  tCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGa                                                                              >  1:27782/1‑140 (MQ=255)                                                           ggTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGAtt                                                                     <  2:64779/140‑1 (MQ=255)                                                            gtcgtcTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTc                                                                    >  1:599614/1‑140 (MQ=255)                                                                         ccTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTTACTCTTTGAAGACGGATTCTGGAGTTTACGAt                                                       <  2:534376/140‑1 (MQ=255)                                                                         ccTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGAt                                                       >  1:998148/1‑140 (MQ=255)                                                                         ccTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGAt                                                       <  1:310934/140‑1 (MQ=255)                                                                           tGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGa                                                     >  2:764708/1‑140 (MQ=255)                                                                           tGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGa                                                     >  2:248836/1‑140 (MQ=255)                                                                            ggCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGac                                                    >  2:816864/1‑140 (MQ=255)                                                                            ggCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGac                                                    >  1:410969/1‑140 (MQ=255)                                                                              ccGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGacta                                                  >  1:205122/1‑140 (MQ=255)                                                                              ccGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGacta                                                  <  1:270776/140‑1 (MQ=255)                                                                              ccGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGacta                                                  >  2:58433/1‑140 (MQ=255)                                                                                gAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGactact                                                <  1:248836/140‑1 (MQ=255)                                                                                 aaGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGActacta                                               <  2:322739/140‑1 (MQ=255)                                                                                  aGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAt                                              >  2:519187/1‑140 (MQ=255)                                                                                   gTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGAt                                                       >  2:492649/1‑130 (MQ=255)                                                                                   gTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGAt                                                       <  1:492649/130‑1 (MQ=255)                                                                                     tCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTAttgt                                           <  2:410969/140‑1 (MQ=255)                                                                                        cgGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGa                                        >  1:678166/1‑140 (MQ=255)                                                                                          gtgtCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACa                                      <  1:519187/140‑1 (MQ=255)                                                                                                aaCTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATc                                <  1:58433/140‑1 (MQ=255)                                                                                                   tGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGc                             <  1:609725/140‑1 (MQ=255)                                                                                                     gCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCg                           >  2:256560/1‑140 (MQ=255)                                                                                                     gCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCg                           <  2:599614/140‑1 (MQ=255)                                                                                                             gcgcgcTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccg                   <  1:600117/140‑1 (MQ=255)                                                                                                              cgcgcTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgc                  <  2:777815/140‑1 (MQ=255)                                                                                                               gcgcTTTCTTTAGCCGTTAATATCCGCCGGTATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgcc                 <  2:640265/140‑1 (MQ=255)                                                                                                               gcgcTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgcc                 <  2:436507/140‑1 (MQ=255)                                                                                                               gcgcTTTCTTTAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTgcccgcc                 <  1:714231/140‑1 (MQ=255)                                                                                                                   tttctttAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTc             <  2:200803/140‑1 (MQ=255)                                                                                                                    ttctttAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCa            <  2:438981/140‑1 (MQ=255)                                                                                                                     tctttAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCAt           <  1:816864/140‑1 (MQ=255)                                                                                                                     tctttAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCAt           <  2:998148/140‑1 (MQ=255)                                                                                                                         tAGCCGTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGa       <  1:194881/140‑1 (MQ=255)                                                                                                                              gTTAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCAtt  <  1:605145/140‑1 (MQ=255)                                                                                                                               ttAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCa    <  2:634156/137‑1 (MQ=255)                                                                                                                               ttAATATCCGCCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCa    >  1:634156/1‑137 (MQ=255)                                                                                                                                        |                                                                                                                                  CGGTCATCAGATGCTGGACGACCTGAAGAAGAAAACCAACCCAGGTTACTCCGCAATTGGTCGTCTGAAAGGCCTGGCCGAAGTTCGCGGTGTCGAACTGGCGATCCAGCGCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTTTGAAGACGGATTCTGGAGTTTACGATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATT  >  NC_000913/4552691‑4552955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑