Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,581,419	A→G	V15A (GTA→GCA)	hsdS ←	type I restriction enzyme EcoKI specificity protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,581,419	0	A	G	100.0%	203.1 / NA	55	V15A (GTA→GCA)	hsdS	type I restriction enzyme EcoKI specificity protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/27);  total (28/27)

AACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTT  >  NC_000913/4581283‑4581553                                                                                                                                         |                                                                                                                                       aaCCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTg                                                                                                                                     <  1:443764/140‑1 (MQ=255)     aaGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGc                                                                                                                                 <  2:498306/140‑1 (MQ=255)      aGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCg                                                                                                                                >  1:801722/1‑140 (MQ=255)       gTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGa                                                                                                                               <  2:587953/140‑1 (MQ=255)       gTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGa                                                                                                                               <  1:939366/140‑1 (MQ=255)                    aaCTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTc                                                                                                                  >  2:401029/1‑140 (MQ=255)                     aCTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTcc                                                                                                                 <  2:796701/140‑1 (MQ=255)                         gCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCa                                                                                                             >  2:963145/1‑140 (MQ=255)                         gCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCa                                                                                                             >  2:211291/1‑140 (MQ=255)                            aTTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCCCCCCTCCGGCAAtt                                                                                                          <  2:987547/140‑1 (MQ=255)                                 gAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTcccc                                                                                                     <  1:354421/140‑1 (MQ=255)                                 gAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTcccc                                                                                                     >  2:136901/1‑140 (MQ=255)                                  aaTATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCg                                                                                                    <  1:401029/140‑1 (MQ=255)                                    tatTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCa                                                                                                  <  2:146857/140‑1 (MQ=255)                                      ttGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACt                                                                                                <  2:606725/140‑1 (MQ=255)                                      ttGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACt                                                                                                <  2:801722/140‑1 (MQ=255)                                         ttCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAt                                                                                             >  2:7439/1‑140 (MQ=255)                                         ttCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCAt                                                                                             >  1:744240/1‑140 (MQ=255)                                            gCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGAAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTcc                                                                                          >  2:756237/1‑140 (MQ=255)                                                          cAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCaaa                                                                            >  1:230574/1‑140 (MQ=255)                                                            aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACg                                                                          >  1:874221/1‑140 (MQ=255)                                                            aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACg                                                                          >  1:482106/1‑140 (MQ=255)                                                            aataatCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACg                                                                          >  2:298880/1‑140 (MQ=255)                                                                   atcTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCttcttc                                                                   >  2:268686/1‑140 (MQ=255)                                                                               aTTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCTCCAAACGCTTCTTCCAGCAACTGACg                                                       >  1:730472/1‑140 (MQ=255)                                                                                          gCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                             >  1:405591/1‑139 (MQ=255)                                                                                          gCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCg                                             <  2:405591/139‑1 (MQ=255)                                                                                               tttttATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCa                                       <  1:963145/140‑1 (MQ=255)                                                                                                    ataCGTTACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  <  2:482106/140‑1 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  >  2:270593/1‑134 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  <  1:270593/134‑1 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  <  2:499321/134‑1 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  >  1:499321/1‑134 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGcccc                            >  2:406487/1‑140 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGcccc                            >  2:349000/1‑140 (MQ=255)                                                                                                          tACTCCTCGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGcccc                            >  1:71063/1‑140 (MQ=255)                                                                                                            ctcctcGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  >  1:459546/1‑132 (MQ=255)                                                                                                            ctcctcGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCAtcgct                                  <  2:459546/132‑1 (MQ=255)                                                                                                               ctcGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGtt                       <  1:268686/140‑1 (MQ=255)                                                                                                                 cGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCa                     <  2:1001727/140‑1 (MQ=255)                                                                                                                 cGGATTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCa                     <  2:749338/140‑1 (MQ=255)                                                                                                                    aTTAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGc                  >  1:275320/1‑140 (MQ=255)                                                                                                                     ttAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCa                 <  2:1000474/140‑1 (MQ=255)                                                                                                                      tAGAGTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCAt                >  2:249913/1‑140 (MQ=255)                                                                                                                       agagTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATc               >  1:694230/1‑140 (MQ=255)                                                                                                                         agTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg             >  2:166194/1‑140 (MQ=255)                                                                                                                         agTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAg             <  1:298880/140‑1 (MQ=255)                                                                                                                          gTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAgc            <  2:45233/140‑1 (MQ=255)                                                                                                                          gTTGTGACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAgc            <  1:211291/140‑1 (MQ=255)                                                                                                                              tgACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCa                 >  2:10826/1‑131 (MQ=255)                                                                                                                              tgACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCa                 <  1:10826/131‑1 (MQ=255)                                                                                                                              tgACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCa              >  1:1040372/1‑134 (MQ=255)                                                                                                                              tgACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCa              <  2:1040372/134‑1 (MQ=255)                                                                                                                              tgACCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCAt        <  1:7439/140‑1 (MQ=255)                                                                                                                                aCCGTAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATcc      <  2:71063/140‑1 (MQ=255)                                                                                                                                    tAGATGCTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGtt  >  1:567991/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       AACCAAGTCCGTAGTATCAAACTTGCCATTCTGAATATTGTTCGCACGGATAAGAGGCAAATAATCATCTTTTAGATAATTTATTGCCTGCTCTTTTTTATACGTTACTCCTCGGATTAGAGTTGTGACCGTAGATACTGGGGCGATAACCCACCCCTCCGGCAATTTCCCCGCACTCATTCCTTCACCCCACCAAACGCTTCTTCCAGCAACTGACGCTGCAAATCGGCCTCATCGCTCGCCCCCAGTTCACGCATCAGCGCATCCAGTT  >  NC_000913/4581283‑4581553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑