Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,589,756	A→G	G508G (GGT→GGC)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,589,756	0	A	G	100.0%	207.7 / NA	58	G508G (GGT→GGC)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/31);  total (27/31)

ATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGC  >  NC_000913/4589624‑4589886                                                                                                                                     |                                                                                                                                   aTACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCAt                                                                                                                             >  1:163789/1‑140 (MQ=255)     agcagGTAGCCCCACAGACCCGCACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAg                                                                                                                         <  2:875704/140‑1 (MQ=255)      gcagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAgc                                                                                                                        >  2:167102/1‑140 (MQ=255)       cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAgcg                                                                                                                       <  2:214743/140‑1 (MQ=255)       cagGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAgcg                                                                                                                       >  1:665955/1‑140 (MQ=255)         ggTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGcgcg                                                                                                                     >  1:560620/1‑140 (MQ=255)          gTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCgg                                                                                                                    <  2:560620/140‑1 (MQ=255)           tAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGt                                                                                                                   <  2:330937/140‑1 (MQ=255)           tAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGt                                                                                                                   >  2:533704/1‑140 (MQ=255)            aGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTc                                                                                                                  >  1:595993/1‑140 (MQ=255)              ccccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAg                                                                                                                >  1:127185/1‑140 (MQ=255)               cccACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAgg                                                                                                               <  1:800511/140‑1 (MQ=255)                 cacaGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGAt                                                                                                             >  2:328062/1‑140 (MQ=255)                 cacaGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGAt                                                                                                             >  2:883562/1‑140 (MQ=255)                 cacaGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGAt                                                                                                             >  1:654357/1‑140 (MQ=255)                 cacaGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGAt                                                                                                             >  1:571816/1‑140 (MQ=255)                     gACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAAc                                                                                                         >  1:297018/1‑140 (MQ=255)                     gACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAAc                                                                                                         >  2:10987/1‑140 (MQ=255)                        ccACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAgg                                                                                                      >  1:1006058/1‑140 (MQ=255)                          acacaGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGc                                                                                                    >  2:220769/1‑140 (MQ=255)                             caGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTc                                                                                                 <  1:347854/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              >  1:491955/1‑140 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  2:571816/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  1:251915/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  2:189688/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  1:533704/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  2:1006058/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              >  1:96711/1‑140 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  2:595993/140‑1 (MQ=255)                                cccGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGaa                                                                                              <  2:665955/140‑1 (MQ=255)                                  cGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCATGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACa                                                                                            >  1:656688/1‑140 (MQ=255)                                        aCCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATAc                                                                                      <  2:96711/140‑1 (MQ=255)                                         ccGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATAcc                                                                                     <  1:31486/140‑1 (MQ=255)                                          cGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAAt                                                                                        <  1:922782/136‑1 (MQ=255)                                          cGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAAt                                                                                        >  2:922782/1‑136 (MQ=255)                                          cGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCg                                                                                    >  1:360625/1‑140 (MQ=255)                                            atgatACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGaa                                                                                  <  2:297018/140‑1 (MQ=255)                                                tACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGa                                                                              <  2:163789/140‑1 (MQ=255)                                                 aCCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGAt                                                                             >  2:27771/1‑140 (MQ=255)                                                   cGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATAc                                                                           >  1:404182/1‑140 (MQ=255)                                                    ggCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATAcc                                                                          >  2:588688/1‑140 (MQ=255)                                                      cAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAg                                                                        <  1:328062/140‑1 (MQ=255)                                                          cAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGc                                                                    <  1:167102/140‑1 (MQ=255)                                                             agaATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCa                                                                 >  1:891296/1‑140 (MQ=255)                                                                     tttttttCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGcc                                                         <  1:10987/140‑1 (MQ=255)                                                                               gAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGgcagca                                               <  2:491955/140‑1 (MQ=255)                                                                                  cGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACtt                                            <  1:795532/140‑1 (MQ=255)                                                                                       tGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTgg                                       <  1:220769/140‑1 (MQ=255)                                                                                          aGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAac                                    <  1:152656/140‑1 (MQ=255)                                                                                              aCCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGt                                <  1:883562/140‑1 (MQ=255)                                                                                                ccAGCATGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTgc                              <  2:656688/140‑1 (MQ=255)                                                                                                 cagcagGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTgcg                             <  1:335693/140‑1 (MQ=255)                                                                                                     agGTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGACAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGAt                         >  2:219922/1‑140 (MQ=255)                                                                                                      ggTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGa                          >  1:959412/1‑138 (MQ=255)                                                                                                      ggTCTTGCAGCATAAAGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGa                          <  2:959412/138‑1 (MQ=255)                                                                                                                     aGCGGCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTc         >  1:567021/1‑140 (MQ=255)                                                                                                                         gCCAGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGgcg     <  2:891296/140‑1 (MQ=255)                                                                                                                            aGAACGGGTGCCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGgcgcgc  <  2:360625/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                   ATACAGCAGGTAGCCCCACAGACCCACACAGCCCGCAGTACCGATGATACCGGCAACCAGAGAATCGGTTTTTTTCAGGAACGGGATGAAGTTACCCAGCAGGTCTTGCAGCATAAAGCGGCCAGAACGGGTACCCGCATCCAGCGCGGTCAGGATGAACAGGGCTTCGAACAGAATACCGAAGTGATACCAGAAGCCCATGTCAGCCATCGGCAGCACTTTGTGGAACACGTGCGCGATACCTACCGCCAGCGTCGGCGCGC  >  NC_000913/4589624‑4589886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑