Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,632,628	A→G	K632K (AAA→AAG)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,632,628	0	A	G	100.0%	207.9 / NA	57	K632K (AAA→AAG)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/29);  total (28/29)

CCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGC  >  NC_000913/4632495‑4632764                                                                                                                                      |                                                                                                                                         ccTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACg                                                                                                                                    <  1:862834/140‑1 (MQ=255)        tGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTtgatga                                                                                                                             <  1:799418/140‑1 (MQ=255)          gCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAgc                                                                                                                           >  1:276140/1‑140 (MQ=255)          gCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAgc                                                                                                                           >  2:824906/1‑140 (MQ=255)            aaCAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAgcgc                                                                                                                         >  1:248638/1‑140 (MQ=255)              cAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGACTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCa                                                                                                                       <  2:188001/140‑1 (MQ=255)                 cgcCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTtgat                                                                                                                               <  1:678114/129‑1 (MQ=255)                 cgcCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTtgat                                                                                                                               >  2:678114/1‑129 (MQ=255)                       gCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATggg                                                                                                              <  2:430057/140‑1 (MQ=255)                            tCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGAcgt                                                                                                         <  2:250585/140‑1 (MQ=255)                                 gCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTa                                                                                                    <  1:972319/140‑1 (MQ=255)                                     tGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGa                                                                                                >  1:381530/1‑140 (MQ=255)                                       gtaTTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATc                                                                                              <  1:991272/138‑1 (MQ=255)                                         aTTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg                                                                                            <  2:280616/140‑1 (MQ=255)                                         aTTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCg                                                                                            <  1:875862/140‑1 (MQ=255)                                           ttGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCa                                                                                          <  2:248638/140‑1 (MQ=255)                                              tCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGAcgt                                                                                                         <  1:116587/122‑1 (MQ=255)                                              tCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGAcgt                                                                                                         >  2:116587/1‑122 (MQ=255)                                              tCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGt                                                                                       <  1:824906/140‑1 (MQ=255)                                               cGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGtt                                                                                      <  1:743738/140‑1 (MQ=255)                                               cGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGtt                                                                                      >  2:564685/1‑140 (MQ=255)                                                       ttCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGc                                                                                           >  2:323849/1‑127 (MQ=255)                                                       ttCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGc                                                                                           <  1:323849/127‑1 (MQ=255)                                                       ttCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGt                                                                                       >  1:634501/1‑131 (MQ=255)                                                       ttCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGt                                                                                       <  2:634501/131‑1 (MQ=255)                                                       ttCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGatat                                                                              >  2:129929/1‑140 (MQ=255)                                                       ttCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGatat                                                                              >  2:542567/1‑140 (MQ=255)                                                        tCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATg                                                                             <  1:436391/140‑1 (MQ=255)                                                             tctcCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATc                                                                        <  1:523095/140‑1 (MQ=255)                                                               tcCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGt                                                                      >  2:517510/1‑140 (MQ=255)                                                               tcCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGt                                                                      >  2:609295/1‑140 (MQ=255)                                                               tcCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGt                                                                      <  1:517510/140‑1 (MQ=255)                                                                 cGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTAc                                                                    >  2:530491/1‑140 (MQ=255)                                                                   agaCGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTAGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTActc                                                                  >  2:831307/1‑140 (MQ=255)                                                                             ttATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGt                                                        <  2:381530/140‑1 (MQ=255)                                                                                       aaCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCgggg                                              >  1:279/1‑140 (MQ=255)                                                                                       aaCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCgggg                                              >  2:320937/1‑140 (MQ=255)                                                                                       aaCGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCgggg                                              >  1:1025079/1‑140 (MQ=255)                                                                                         cGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGa                                            >  1:838748/1‑140 (MQ=255)                                                                                         cGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGa                                            >  1:152422/1‑140 (MQ=255)                                                                                          gTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAg                                           >  1:988432/1‑140 (MQ=255)                                                                                                  ttATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTg                                   <  2:1025079/140‑1 (MQ=255)                                                                                                  ttATGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTg                                   <  1:564685/140‑1 (MQ=255)                                                                                                     tGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCtt                                <  2:838748/140‑1 (MQ=255)                                                                                                     tGACGCTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCtt                                <  1:542567/140‑1 (MQ=255)                                                                                                          cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTccccc                           >  2:113235/1‑140 (MQ=255)                                                                                                          cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTccccc                           >  1:590799/1‑140 (MQ=255)                                                                                                          cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTccccc                           >  2:762540/1‑140 (MQ=255)                                                                                                          cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTc                               >  2:1035230/1‑136 (MQ=255)                                                                                                          cTTACTACCGCTATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTc                               <  1:1035230/136‑1 (MQ=255)                                                                                                                     tATTTCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATgg                <  2:152422/140‑1 (MQ=255)                                                                                                                       tttCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCGGCAAAAAATGGCg              >  2:668661/1‑140 (MQ=255)                                                                                                                         tCATGGGGGATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGAc            <  1:831307/140‑1 (MQ=255)                                                                                                                               gggATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACAtatta      <  2:549750/140‑1 (MQ=255)                                                                                                                                ggATAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACAtattat     <  1:320937/140‑1 (MQ=255)                                                                                                                                  aTAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATgg   >  1:88613/1‑140 (MQ=255)                                                                                                                                   tAAGCCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGc  >  1:356910/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CCTGGCTTGGCAACAGCGCCGGGCGTATCGACGCAGTGGCATTTGTCGAGAGTATTCCATTCTCCGAGACGCGCGGTTATGTGAAGAACGTGCTGGCTTATGACGCTTACTACCGCTATTTCATGGGGGATAAACCGACGTTGATGAGCGCCACGGAATGGGGACGTCGTTACTGATCCGCACGTTTATGATATGCTATCGTACTCTTTAGCGAGTACAACCGGGGGAGGCATTTTGCTTCCCCCGCTAACAATGGCGACATATTATGGC  >  NC_000913/4632495‑4632764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑