Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	309,752	A→G	V92A (GTG→GCG)	ecpB ←	putative fimbrial chaperone EcpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	309,752	0	A	G	100.0%	226.7 / NA	62	V92A (GTG→GCG)	ecpB	putative fimbrial chaperone EcpB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/28);  total (34/28)

CGATGCGGGCGGAAGCAGTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGAC  >  NC_000913/309615‑309884                                                                                                                                          |                                                                                                                                      cGATGCGGGCGGAAGCAGTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCt                                                                                                                                     >  2:157002/1‑140 (MQ=255)   aTGCGGGCGGAAGCAGTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACCGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTc                                                                                                                                   >  2:935502/1‑140 (MQ=255)    tGCGGGCGGAAGCAGTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCg                                                                                                                                  <  1:161720/140‑1 (MQ=255)                  gTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑ggg                                                                                                                   <  1:379277/140‑1 (MQ=255)                   tGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGt                                                                                                                  >  2:299802/1‑140 (MQ=255)                       cacaGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTagca                                                                                                              >  2:78013/1‑140 (MQ=255)                         caGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTagcagc                                                                                                            >  2:816570/1‑140 (MQ=255)                                gCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTgg                                                                                                     <  1:784228/140‑1 (MQ=255)                                      ggCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGgag                                                                                               >  2:207664/1‑140 (MQ=255)                                                cgcTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCaa                                                                                     <  2:446092/140‑1 (MQ=255)                                                     cgcCTCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCAt                                                                                <  1:591189/140‑1 (MQ=255)                                                       cATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATcc                                                                              >  1:64368/1‑140 (MQ=255)                                                         tCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCgg                                                                            <  2:64368/140‑1 (MQ=255)                                                            cTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCtt                                                                         <  2:290991/140‑1 (MQ=255)                                                                 ggCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCa                                                                    <  1:540883/140‑1 (MQ=255)                                                                   ccTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCAtt                                                                  >  2:902711/1‑140 (MQ=255)                                                                    cTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTg                                                                 >  1:225732/1‑140 (MQ=255)                                                                      gATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTgag                                                               >  1:861476/1‑140 (MQ=255)                                                                       aTCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTgaga                                                              <  2:861476/140‑1 (MQ=255)                                                                       aTCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTgaga                                                              >  2:1013428/1‑140 (MQ=255)                                                                       aTCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGGGAGTAGCAACTCATCCGGCTTGTCCATTtaga                                                              >  1:639247/1‑140 (MQ=255)                                                                        tCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGAt                                                             <  1:1013428/140‑1 (MQ=255)                                                                           aaCCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAAc                                                          >  2:249500/1‑140 (MQ=255)                                                                            aCCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAAcc                                                         >  1:406810/1‑140 (MQ=255)                                                                            aCCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAAcc                                                         >  1:350560/1‑140 (MQ=255)                                                                               aGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGc                                                      <  2:96724/140‑1 (MQ=255)                                                                                gACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGcc                                                     <  2:735778/140‑1 (MQ=255)                                                                                 aCAATGCGGTAGTAGCGCTCTTTTTTATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGccc                                                    >  2:120063/1‑140 (MQ=255)                                                                                 aCAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGccc                                                    >  1:833982/1‑140 (MQ=255)                                                                                 aCAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGccc                                                    >  1:669959/1‑140 (MQ=255)                                                                                 aCAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGccc                                                    >  2:104682/1‑140 (MQ=255)                                                                                       cGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCgtcgtc                                              <  1:230524/140‑1 (MQ=255)                                                                                            gtagCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCg                                         <  2:591350/140‑1 (MQ=255)                                                                                              agCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGt                                       <  1:367029/140‑1 (MQ=255)                                                                                                  ctctTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAag                                   >  1:328358/1‑140 (MQ=255)                                                                                                    ctTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGCGGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAaga                                  >  1:707652/1‑140 (MQ=255)                                                                                                    ctTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGTTTGTCCATTGAGATAACCTGCCCGTCGGCAAGCGGTGAaggg                                 >  2:964542/1‑138 (MQ=255)                                                                                                    ctTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAagag                                 >  1:863449/1‑140 (MQ=255)                                                                                                      ttttCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAagagag                               <  2:225732/140‑1 (MQ=255)                                                                                                      ttttCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAagagag                               <  1:249500/140‑1 (MQ=255)                                                                                                      ttttCATCTGCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAagagag                               <  1:299802/140‑1 (MQ=255)                                                                                                               gCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCgag                      >  1:665277/1‑140 (MQ=255)                                                                                                               gCGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCgag                      >  2:299093/1‑140 (MQ=255)                                                                                                                cGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCgaga                     <  2:669959/140‑1 (MQ=255)                                                                                                                cGGGTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCgaga                     <  2:665277/140‑1 (MQ=255)                                                                                                                  ggTCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACg                   <  1:964542/140‑1 (MQ=255)                                                                                                                    tCCCTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGa                 <  1:207664/140‑1 (MQ=255)                                                                                                                       cTTATAGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGatat              >  1:422749/1‑140 (MQ=255)                                                                                                                           taGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGGGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGAt          <  2:763640/140‑1 (MQ=255)                                                                                                                           taGAAGAAGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGAt          <  2:833982/140‑1 (MQ=255)                                                                                                                             gaagaaGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGAt          <  2:601177/138‑1 (MQ=255)                                                                                                                             gaagaaGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGAt          >  1:601177/1‑138 (MQ=255)                                                                                                                             gaagaaGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATaa        >  2:668447/1‑140 (MQ=255)                                                                                                                             gaagaaGCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATaa        >  2:835865/1‑140 (MQ=255)                                                                                                                              aagaagCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAg       >  2:244378/1‑140 (MQ=255)                                                                                                                                gaagCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGc     <  2:422749/140‑1 (MQ=255)                                                                                                                                 aagCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCg    >  1:347688/1‑140 (MQ=255)                                                                                                                                  agCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGa   >  2:456787/1‑140 (MQ=255)                                                                                                                                  agCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGa   <  1:299093/140‑1 (MQ=255)                                                                                                                                  agCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGa   >  1:78702/1‑140 (MQ=255)                                                                                                                                   gCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGAc  >  2:701563/1‑140 (MQ=255)                                                                                                                                   gCGGATCGCTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGAc  <  1:816570/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      CGATGCGGGCGGAAGCAGTGGCCACAGCGCTGCGGTTGGCATTATCGCGCTGCGCATCACTGAGGGCCTGATCAAACCAGACAATGCGGTAGTAGCGCTCTTTTTCATCTGCGGGTCCCTTATAGAAGAAGCGGATCACTTCGCTGGCTTGGGC‑GGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGAC  >  NC_000913/309615‑309884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑