Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	457,810	G→A	A129T (GCT→ACT)	clpX →	ATP‑dependent Clp protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	457,810	0	G	A	100.0%	176.4 / NA	51	A129T (GCT→ACT)	clpX	ATP‑dependent Clp protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

GGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGCGACTACGA  >  NC_000913/457674‑457940                                                                                                                                         |                                                                                                                                   ggCGAAAAAAGTGCTGGCGGTCGCGGGATAAAACCATTACAAAAGGCTGCGCCACGGCGATAACAGCAATGGCGGCGAGGTGGGGAAAAAGAAAATTCTGCTGATCGGGCCGACCCGGTCCGGGAAAAAGCTgctgactg                                                                                                                                 >  2:1040236/1‑140 (MQ=255)      aaaaaGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACg                                                                                                                            >  1:838032/1‑140 (MQ=255)             gCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGgcgc                                                                                                                     <  2:424164/140‑1 (MQ=255)              cTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGgcgcg                                                                                                                    >  1:821096/1‑140 (MQ=255)               tGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGgcgcgc                                                                                                                   <  2:838032/140‑1 (MQ=255)                         ggTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATg                                                                                                         <  2:481381/140‑1 (MQ=255)                         ggTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATg                                                                                                         <  1:506388/140‑1 (MQ=255)                                aaCCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGtt                                                                                                  <  1:389565/140‑1 (MQ=255)                                  ccATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCa                                                                                                >  1:627696/1‑140 (MQ=255)                                   cATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCAc                                                                                               >  1:851016/1‑140 (MQ=255)                                   cATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTGCCGTTCAc                                                                                               >  2:594398/1‑140 (MQ=255)                                     ttACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGt                                                                                                        >  2:614155/1‑129 (MQ=255)                                     ttACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGt                                                                                                        <  1:614155/129‑1 (MQ=255)                                     ttACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCa                                                                                             >  1:378780/1‑140 (MQ=255)                                       aCAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATg                                                                                           >  2:730929/1‑140 (MQ=255)                                       aCAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATg                                                                                           >  2:876051/1‑140 (MQ=255)                                       aCAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATg                                                                                           >  1:331737/1‑140 (MQ=255)                                       aCAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATg                                                                                           >  1:1014923/1‑140 (MQ=255)                                          aaCGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGcc                                                                                        >  2:641178/1‑140 (MQ=255)                                            cGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGa                                                                                      <  1:588061/140‑1 (MQ=255)                                            cGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGa                                                                                      <  1:52076/140‑1 (MQ=255)                                             gTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGAc                                                                                     >  1:62246/1‑140 (MQ=255)                                             gTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGAc                                                                                     >  2:88239/1‑140 (MQ=255)                                              tCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGAcg                                                                                    <  2:1014923/140‑1 (MQ=255)                                                       cGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTacac                                                                           >  1:795055/1‑140 (MQ=255)                                                           gATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGAc                                                                       <  1:944879/140‑1 (MQ=255)                                                            aTACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGAcc                                                                      <  2:795055/140‑1 (MQ=255)                                                              aCCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGa                                                                    <  1:384033/140‑1 (MQ=255)                                                                 aGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGc                                                                 >  2:194526/1‑140 (MQ=255)                                                                 aGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGc                                                                 <  2:378780/140‑1 (MQ=255)                                                                  gCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGcc                                                                <  2:851016/140‑1 (MQ=255)                                                                         cGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg                                                         >  2:173331/1‑140 (MQ=255)                                                                         cGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg                                                         >  1:768567/1‑140 (MQ=255)                                                                         cGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg                                                         >  2:263784/1‑140 (MQ=255)                                                                         cGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg                                                         >  1:511757/1‑140 (MQ=255)                                                                         cGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtg                                                         >  2:745243/1‑140 (MQ=255)                                                                          gTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAtgt                                                        <  1:194526/140‑1 (MQ=255)                                                                           tCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAATGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGcc                                                                >  2:285837/1‑131 (MQ=255)                                                                           tCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAATGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGcc                                                                <  1:285837/131‑1 (MQ=255)                                                                                  gggCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAt                                                          >  2:416956/1‑130 (MQ=255)                                                                                  gggCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTAt                                                          <  1:416956/130‑1 (MQ=255)                                                                                  gggCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAg                                                <  2:821096/140‑1 (MQ=255)                                                                                  gggCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAg                                                <  1:641178/140‑1 (MQ=255)                                                                                              cATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAca                                    <  2:62246/140‑1 (MQ=255)                                                                                              cATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAca                                    <  2:511757/140‑1 (MQ=255)                                                                                               aTTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAAcat                                   <  1:738944/140‑1 (MQ=255)                                                                                                   tgctgATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCAtt                               <  1:88239/140‑1 (MQ=255)                                                                                                               cGACCGGTTCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTg                   >  2:233299/1‑140 (MQ=255)                                                                                                                      ttCCGGTAAAACGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAAt            >  2:890127/1‑140 (MQ=255)                                                                                                                               aaCGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGCGACTACg   <  2:768567/140‑1 (MQ=255)                                                                                                                               aaCGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGCGACTACg   <  1:1040236/140‑1 (MQ=255)                                                                                                                                aCGCTGCTGACTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGCGACTACGa  <  1:173331/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GGCGAAAAAAGTGCTGGCGGTCGCGGTATACAACCATTACAAACGTCTGCGCAACGGCGATACCAGCAATGGCGTCGAGTTGGGCAAAAGTAACATTCTGCTGATCGGTCCGACCGGTTCCGGTAAAACGCTGCTGGCTGAAACGCTGGCGCGCCTGCTGGATGTTCCGTTCACCATGGCCGACGCGACTACACTGACCGAAGCCGGTTATGTGGGTGAAGACGTTGAAAACATCATTCAGAAGCTGTTGCAGAAATGCGACTACGA  >  NC_000913/457674‑457940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑