Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	31,834	A→G	T340A (ACT→GCT)	carB →	carbamoyl phosphate synthetase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	31,834	0	A	G	100.0%	72.5 / NA	21	T340A (ACT→GCT)	carB	carbamoyl phosphate synthetase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (1/20);  total (1/20)

CTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGT  >  NC_000913/31699‑31958                                                                                                                                        |                                                                                                                             cTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTgg                                                                                                                          <  1:1027040/140‑1 (MQ=255) cTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTgg                                                                                                                          <  1:156614/140‑1 (MQ=255) cTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTgg                                                                                                                          <  2:591689/140‑1 (MQ=255)    aTTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTACACCCTCGACGAACTGATGAACGACATCGCTGGCgg                                                                                                                       >  1:374811/1‑140 (MQ=255)      tGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGAc                                                                                                                     <  2:842305/140‑1 (MQ=255)                  gAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGGGGGGTACACCCCCCACGAACTGATGAACGACATCGCTGGCGGGCGGAATCCCGccc                                                                                                         >  2:792541/1‑139 (MQ=255)                   aaCCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTTCACCCTCGACGAAATGATGAAAGACATCGCTGGCGGGCGGACTcccggccc                                                                                                        >  2:658019/1‑134 (MQ=255)                             tgtCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTTGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCg                                                                                              <  2:843576/140‑1 (MQ=255)                                  cGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCAt                                                                                         <  2:37243/140‑1 (MQ=255)                                         cGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGGTACACCCCCCGCGAAACGATGAACGACATCGCTGGCGGGGGGAATCCCGGCCCCCTCCAGCCGTCCCTCCACCAt                                                                                  >  2:769336/1‑140 (MQ=255)                                               tGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAACCCCGACGAACTGGAGGACGGCATCGCCGGGGGGCGTAATCCCGCCCCCCTCCAGGCGGCCCACGACCAttggggt                                                                            >  2:163215/1‑134 (MQ=255)                                                     cGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTaaa                                                                      <  1:889925/140‑1 (MQ=255)                                                                        gATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCg                                                   <  1:625856/140‑1 (MQ=255)                                                                        gATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCCCGACGAACCGGTGAACGACAACCCTGGCGGGCCTACTCCGGCCCCCCTTGAGCCCTCCCTCCACTATTTGGTTTCTAAAATTTCTCCCTTCCAATTCg                                                   >  2:73063/1‑140 (MQ=255)                                                                            gCTAAAGTGGCGGCGAAACTGGCGGCGGGGTTCACCCCCGGCGAACCGGTGAAAGACATCACTGGCGGGCCTACTCCAGCCCCCCCCGAGCCCTCCATCGACCATTTTGTTAATTAAAATTCTCGCGTCAACCTTGaaaa                                               >  2:625856/1‑140 (MQ=255)                                                                                aaGTGGCGGCGAAACTGGCGGTGGGGTACACCCCCGAAGGAATGATGGACGACATCGCTGGCGGACGTACTCCCGCCCCCCTCGGGGCGTCCATCGACTATGTGGGTTTTAAAAATTCCCGCTTTAACTTTGAAAAAATc                                           >  2:944428/1‑140 (MQ=255)                                                                                  gTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGc                                         <  1:530200/140‑1 (MQ=255)                                                                                    ggcggcGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCg                                       <  1:792541/140‑1 (MQ=255)                                                                                    ggcggcGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCg                                       <  1:51556/140‑1 (MQ=255)                                                                                     gcggcgAAACTGGCGGGGGGGTTCACCCCCCACGAACTGATGAACGACATCGCTGGCGGGCGGACTCCCGCCCCCCTCGAGCCCTCCCTCGACTATGTGGGTACTAAAAATTCTCCCTTCAACCTCGAAAAAATCGcccg                                      >  2:819895/1‑138 (MQ=255)                                                                                      cggcgAAACTGGCGGTGGGGTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGGCGGACTCCCGCCTCCCTCGGGCCGTCCATCGACTATGTGGTTTCTAAAAATTCTCGCCTTAACTTCGAAAAAATCGcccgg                                     >  2:530200/1‑137 (MQ=255)                                                                                        gcgAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGc                                   <  1:755939/140‑1 (MQ=255)                                                                                             aCTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACg                              <  1:73063/140‑1 (MQ=255)                                                                                             aCTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACg                              <  1:265296/140‑1 (MQ=255)                                                                                             aCTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGAGCTAACg                              <  1:1004817/140‑1 (MQ=255)                                                                                                      gggTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGa                     <  1:769336/140‑1 (MQ=255)                                                                                                      gggTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGa                     <  1:163215/140‑1 (MQ=255)                                                                                                      gggTTACACCCTCGACGAACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGa                     <  1:787450/140‑1 (MQ=255)                                                                                                                    acgaACTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGaaa       <  1:944428/140‑1 (MQ=255)                                                                                                                        aCTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCgg   <  2:374811/140‑1 (MQ=255)                                                                                                                         cTGATGAACGACATCGCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGt  >  1:750018/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                             CTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTAACGACCGTCTGACCACTCAGATGAAATCGGT  >  NC_000913/31699‑31958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑