Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,227	A→G	T114A (ACG→GCG)	glxK →	glycerate 2‑kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,227	0	A	G	100.0%	119.3 / NA	36	T114A (ACG→GCG)	glxK	glycerate 2‑kinase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/21);  total (15/21)

CGATGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTGTGCGTTTCCTTGATGCCGACGGTCA  >  NC_000913/542090‑542363                                                                                                                                          |                                                                                                                                         cGATGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCg                                                                                                                                        >  1:505676/1‑140 (MQ=255)  gATGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCgg                                                                                                                                       <  2:126539/140‑1 (MQ=255)   aTGGGCGAAAAAGGGAATGCTTTTTATGGCCTTACAGGCGACGGGAAAAGGGCGGTGATTGATATGGTGGGAGCAAGTGACATGATGCGGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCggg                                                                                                                                      <  2:72165/140‑1 (MQ=255)    tGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCgggg                                                                                                                                     >  1:213976/1‑140 (MQ=255)                      cTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATgcg                                                                                                                   <  2:505676/140‑1 (MQ=255)                        ttttATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCt                                                                                                                 >  1:154183/1‑140 (MQ=255)                         tttATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTg                                                                                                                <  1:428112/140‑1 (MQ=255)                              ggCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATaa                                                                                                           <  2:407251/140‑1 (MQ=255)                                  ttACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGAc                                                                                                       <  2:1028995/140‑1 (MQ=255)                                  ttACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGAc                                                                                                       >  1:139513/1‑140 (MQ=255)                                  ttACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGAc                                                                                                       <  1:179719/140‑1 (MQ=255)                                   tACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATgcg                                                                                                                   <  1:444345/127‑1 (MQ=255)                                   tACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATgcg                                                                                                                   >  2:444345/1‑127 (MQ=255)                                   tACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAACGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACa                                                                                                      >  1:858952/1‑140 (MQ=255)                                    cccGGCGAGGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCAGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACAt                                                                                                     <  2:491738/139‑1 (MQ=255)                                       ggCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCg                                                                                                  <  2:213976/140‑1 (MQ=255)                                         cGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGcc                                                                                                <  1:853194/140‑1 (MQ=255)                                         cGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGcc                                                                                                <  2:154183/140‑1 (MQ=255)                                          gACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCg                                                                                                  >  2:584563/1‑137 (MQ=255)                                          gACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCg                                                                                                  <  1:584563/137‑1 (MQ=255)                                            cGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCata                                                                                             <  2:792902/140‑1 (MQ=255)                                              ggAAAACGGCGGTGGTTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGGCGCGCCTGAAAAGCGTAATCCGCTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCAtatt                                                                                           >  2:288632/1‑140 (MQ=255)                                                     ggcggTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCg                                                                                    <  1:50198/140‑1 (MQ=255)                                                       cggTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGGTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGc                                                                                  >  1:154848/1‑140 (MQ=255)                                                                    ggcggcAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTg                                                                     <  1:288632/140‑1 (MQ=255)                                                                           gcaAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACggg                                                              >  2:859604/1‑139 (MQ=255)                                                                              aGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGa                                                           <  1:985865/140‑1 (MQ=255)                                                                                       aTGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTAGACGGCGGTAt                                                  >  1:618929/1‑140 (MQ=255)                                                                                             gTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcgg                                                        >  2:339524/1‑128 (MQ=255)                                                                                             gTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGAcgg                                                        <  1:339524/128‑1 (MQ=255)                                                                                             gTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTAGACGGCGGTATGGGCAt                                            <  2:618929/140‑1 (MQ=255)                                                                                                 cgcCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTa                                                   >  2:617173/1‑129 (MQ=255)                                                                                                 cgcCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTa                                                   <  1:617173/129‑1 (MQ=255)                                                                                                             gTAATCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGgt                            <  2:858952/140‑1 (MQ=255)                                                                                                                 tCCGTTGCTGGCCTCCAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTGTGc                        >  1:532654/1‑140 (MQ=255)                                                                                                                               ccAGTTTTGGTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTGTGCGTTTCCTTGATGcc          >  2:6843/1‑140 (MQ=255)                                                                                                                                       ggTGCGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTGTGCGTTTCCTTGATGCCGACGGTCa  >  2:56827/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         CGATGGGCGAAAAAGTGAATGCTTTTTATGGCCTTACCGGCGACGGGAAAACGGCGGTGATTGAGATGGCGGCAGCAAGTGGCCTGATGCTGGTCGCGCCTGAAAAGCGTAATCCGTTGCTGGCCTCCAGTTTTGGTACGGGGGAGTTAATTCGTCATGCGCTGGATAACGACATTCGCCATATTATTCTCGGCATTGGCGGCAGTGCGACGGTCGACGGCGGTATGGGCATGGCGCAGGCGCTCGGTGTGCGTTTCCTTGATGCCGACGGTCA  >  NC_000913/542090‑542363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑