Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	603,772	G→A	R298C (CGC→TGC)	ybdG ←	miniconductance mechanosensitive channel YbdG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	603,772	0	G	A	100.0%	208.3 / NA	58	R298C (CGC→TGC)	ybdG	miniconductance mechanosensitive channel YbdG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/30);  total (28/30)

ACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACA  >  NC_000913/603638‑603900                                                                                                                                       |                                                                                                                                 aCGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTgg                                                                                                                             <  1:1043262/140‑1 (MQ=255)     aTCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTaa                                                                                                                         >  1:300474/1‑140 (MQ=255)       ccGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAAt                                                                                                                       >  2:369425/1‑140 (MQ=255)        cgcgGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATa                                                                                                                      >  1:101755/1‑140 (MQ=255)        cgcgGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATa                                                                                                                      <  2:101755/140‑1 (MQ=255)        cgcgGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATa                                                                                                                      >  1:58108/1‑140 (MQ=255)                 ttACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTaa                                                                                                             >  2:955382/1‑140 (MQ=255)                           atTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTGCACTCATTAATTTCCTGATGGAAGCTGGTTAAATAAGGCTTTAACAAATGCGCt                                                                                                   >  1:664410/1‑140 (MQ=255)                           atTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCt                                                                                                   <  2:571444/140‑1 (MQ=255)                                   aGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAg                                                                                           >  1:616696/1‑140 (MQ=255)                                       aGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGt                                                                                       <  1:369425/140‑1 (MQ=255)                                         gCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTg                                                                                     >  1:936792/1‑140 (MQ=255)                                            cGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATATGGCTTTAACAAATGCGCTTTATTCAGACGTTGCAt                                                                                  <  2:300474/140‑1 (MQ=255)                                               aaGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTc                                                                               >  1:184363/1‑140 (MQ=255)                                                  gTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTc                                                                            <  1:894430/140‑1 (MQ=255)                                                  gTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTc                                                                            >  1:818745/1‑140 (MQ=255)                                                   ttCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCt                                                                           >  1:149010/1‑140 (MQ=255)                                                           ttGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAg                                                                   <  2:616696/140‑1 (MQ=255)                                                             ggTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaa                                                                 >  1:605029/1‑140 (MQ=255)                                                             ggTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaa                                                                 >  1:641695/1‑140 (MQ=255)                                                              gTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGaaa                                                                <  2:605029/140‑1 (MQ=255)                                                                cATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAAc                                                              >  2:146773/1‑140 (MQ=255)                                                                   tCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGaa                                                           >  1:360125/1‑140 (MQ=255)                                                                      gcgcAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATAc                                                        <  1:955382/140‑1 (MQ=255)                                                                      gcgcAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATAc                                                        <  1:593726/140‑1 (MQ=255)                                                                          aGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTGCACTCATTAATTTCCTGATGGAAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGt                                                    <  2:664410/140‑1 (MQ=255)                                                                             tttAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGAc                                                 <  2:936792/140‑1 (MQ=255)                                                                              ttAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACa                                                <  2:360125/140‑1 (MQ=255)                                                                                aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                              <  1:42153/140‑1 (MQ=255)                                                                                aaTACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                              <  2:1025253/140‑1 (MQ=255)                                                                                     cGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatac                                         <  2:637190/140‑1 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                              >  2:601742/1‑134 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                              <  2:524164/134‑1 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                              >  1:524164/1‑134 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATc                                              <  1:601742/134‑1 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                        >  2:155012/1‑140 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                        >  2:603364/1‑140 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                        >  1:710947/1‑140 (MQ=255)                                                                                      gACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatact                                        >  2:49410/1‑140 (MQ=255)                                                                                       aCTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactg                                       <  2:504836/140‑1 (MQ=255)                                                                                       aCTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactg                                       >  1:304526/1‑140 (MQ=255)                                                                                        cTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactga                                      <  2:304526/140‑1 (MQ=255)                                                                                         tCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgat                                     <  2:1013584/140‑1 (MQ=255)                                                                                           cGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatac                                   <  1:905675/140‑1 (MQ=255)                                                                                            gTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatact                                  <  2:184363/140‑1 (MQ=255)                                                                                             tAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCGatactgatactg                                 <  1:566801/140‑1 (MQ=255)                                                                                             tAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatactg                                 >  2:956782/1‑140 (MQ=255)                                                                                             tAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAatactgatactg                                 >  2:168281/1‑140 (MQ=255)                                                                                              aGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTgc                                <  1:817008/140‑1 (MQ=255)                                                                                                       cTGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATAc                       >  1:856107/1‑140 (MQ=255)                                                                                                        tGGCGATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACg                      <  2:641695/140‑1 (MQ=255)                                                                                                            gATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTcgc                  <  2:58108/140‑1 (MQ=255)                                                                                                            gATTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTcgc                  <  1:168281/140‑1 (MQ=255)                                                                                                             aTTCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTcgcc                 >  1:242638/1‑140 (MQ=255)                                                                                                               tCCACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTcgcccg               >  1:808329/1‑140 (MQ=255)                                                                                                                ccACTCATTAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTcgcccgc              <  1:956782/140‑1 (MQ=255)                                                                                                                        tAATTTCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCt      >  1:279391/1‑140 (MQ=255)                                                                                                                            ttCCTGATGGCAGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACa  <  2:710947/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                 ACGAATCCGCGGATGGTTACGCAGATATTCGTTCAGATAGGCACGAAAGGTTCCAATATTGGTCATTCGGCGCAGATTTAATACCGACTCCGTAGAACCTTGCTGGCGATTCCACTCATTAATTTCCTGATGGCGGCTGGTTAAATAAGGCTTTAACAAATGCGCTTTATTCAGACGTTGCATTTCGTCTTCATCAAGAAAACGAATACTGGTGACATCAATACTGATACTGCGCTTAATACGTCGCCCGCCAGATGCTGACA  >  NC_000913/603638‑603900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑