Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	56,246	T→C	E288E (GAA→GAG)	lptD ←	lipopolysaccharide assembly protein LptD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	56,246	0	T	C	100.0%	232.5 / NA	64	E288E (GAA→GAG)	lptD	lipopolysaccharide assembly protein LptD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/33);  total (31/33)

ACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGA  >  NC_000913/56109‑56382                                                                                                                                          |                                                                                                                                         aCCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTc                                                                                                                                        <  2:89174/140‑1 (MQ=255)           aGTCGACGTTGAAACGCCACACCTGATCCATGACCCC‑GAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAg                                                                                                                             >  2:876461/1‑140 (MQ=255)                aCGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgcc                                                                                                                         <  1:861678/140‑1 (MQ=255)                aCGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgcc                                                                                                                         >  1:743241/1‑140 (MQ=255)                 cGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgccc                                                                                                                        >  2:350/1‑140 (MQ=255)                  gTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGAGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCACGCCAGcgcccg                                                                                                                       >  2:573430/1‑140 (MQ=255)                   ttGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgcccgc                                                                                                                      <  1:1004368/140‑1 (MQ=255)                    tGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgcccgcc                                                                                                                     >  1:312260/1‑140 (MQ=255)                      aaaCGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgcccgc                                                                                                                      <  2:726503/137‑1 (MQ=255)                      aaaCGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGcgcccgc                                                                                                                      >  1:726503/1‑137 (MQ=255)                       aaCGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTgg                                                                                                                  <  2:918905/140‑1 (MQ=255)                         cGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGga                                                                                                                >  2:1005350/1‑140 (MQ=255)                          gCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTggg                                                                                                                 <  2:61066/138‑1 (MQ=255)                          gCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTggg                                                                                                                 >  1:61066/1‑138 (MQ=255)                           ccACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGgaga                                                                                                              >  1:9725/1‑140 (MQ=255)                             acacCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAgg                                                                                                            >  2:893747/1‑140 (MQ=255)                                        aTGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTc                                                                                                 >  1:490100/1‑140 (MQ=255)                                         tGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCg                                                                                                <  2:785153/140‑1 (MQ=255)                                          gACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGt                                                                                               <  1:30289/140‑1 (MQ=255)                                           aCCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGtt                                                                                              <  2:9725/140‑1 (MQ=255)                                              ccGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTtctc                                                                                           <  1:906563/140‑1 (MQ=255)                                              ccGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTtctc                                                                                           <  1:500283/140‑1 (MQ=255)                                               cGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTcc                                                                                          >  1:693310/1‑140 (MQ=255)                                                 gAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCca                                                                                        >  1:422152/1‑140 (MQ=255)                                                      gTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACatga                                                                                   >  1:380671/1‑140 (MQ=255)                                                       ttCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACatgat                                                                                  <  2:694439/140‑1 (MQ=255)                                                       ttCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACatgat                                                                                  >  1:694439/1‑140 (MQ=255)                                                        tCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACatgatg                                                                                 <  2:312260/140‑1 (MQ=255)                                                                aaTAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCacg                                                                         <  2:814269/140‑1 (MQ=255)                                                                aaTAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCacg                                                                         <  2:490100/140‑1 (MQ=255)                                                                aaTAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCacg                                                                         >  2:51160/1‑140 (MQ=255)                                                                  tAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCACAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCacgac                                                                       <  2:968047/140‑1 (MQ=255)                                                                    aCCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCAcgacga                                                                     >  2:757394/1‑140 (MQ=255)                                                                    aCCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCAcgacga                                                                     >  1:722518/1‑140 (MQ=255)                                                                     ccAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGAt                                                                    <  2:1022875/140‑1 (MQ=255)                                                                     ccAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGAt                                                                    <  2:422152/140‑1 (MQ=255)                                                                     ccAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGAt                                                                    <  2:693310/140‑1 (MQ=255)                                                                        acgacgTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCa                                                                 >  2:544053/1‑140 (MQ=255)                                                                                      cATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTg                                                   >  2:7653/1‑140 (MQ=255)                                                                                           ttCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggt                                              <  1:893747/140‑1 (MQ=255)                                                                                             cGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGAtggtgg                                            <  2:380671/140‑1 (MQ=255)                                                                                               ggTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCa                                          >  1:451081/1‑140 (MQ=255)                                                                                                     cATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCata                                    >  1:969641/1‑140 (MQ=255)                                                                                                          tCATAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTgg                               >  2:760215/1‑140 (MQ=255)                                                                                                            aTAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcg                             <  1:573430/140‑1 (MQ=255)                                                                                                            aTAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcg                             <  1:499061/140‑1 (MQ=255)                                                                                                             tAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcgc                            <  1:1005350/140‑1 (MQ=255)                                                                                                             tAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcgc                            >  2:411007/1‑140 (MQ=255)                                                                                                             tAGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGgcgc                            <  1:778293/140‑1 (MQ=255)                                                                                                              aGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGcgcg                           <  1:876461/140‑1 (MQ=255)                                                                                                              aGACTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGcgcg                           <  1:760215/140‑1 (MQ=255)                                                                                                                aCTTTATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGCAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGAt                         >  2:780398/1‑140 (MQ=255)                                                                                                                  tttATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCTATATTTGGCGCGATGt                       >  1:683292/1‑140 (MQ=255)                                                                                                                    tATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTc                     <  1:411007/140‑1 (MQ=255)                                                                                                                    tATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTc                     >  2:246904/1‑140 (MQ=255)                                                                                                                    tATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTc                     >  2:1012039/1‑140 (MQ=255)                                                                                                                    tATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTc                     >  1:458935/1‑140 (MQ=255)                                                                                                                    tATCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTc                     >  2:967426/1‑140 (MQ=255)                                                                                                                     aTCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTcc                    >  2:227649/1‑140 (MQ=255)                                                                                                                     aTCTGAAGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTcc                    <  1:967426/140‑1 (MQ=255)                                                                                                                          aaGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGAt                         >  2:790612/1‑130 (MQ=255)                                                                                                                          aaGGCAGATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGAt                         <  1:790612/130‑1 (MQ=255)                                                                                                                                gATAGTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGc         <  2:458935/140‑1 (MQ=255)                                                                                                                                    gTCCAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGt     <  1:544053/140‑1 (MQ=255)                                                                                                                                       cAGCTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGa  <  2:451081/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGACCCCGGAGTGGTTCCAGTAGAATAACCAACGACGTGAACTGTCATCGTTCGGGTGTTCATCTTCATAGACTTTATCTGAAGGCAGATAGTCCAGTTCCATCAAGCCAGCGCCCGCCTGGGAGAGGTAGCGGAATTCGTTCTCCCACATGATGTTGCCACGACGATGCATATAATGCGGCGTGATGGTGGCATCCATATTTGGCGCGATGTTCCAGTAATATGGCAGGTAGA  >  NC_000913/56109‑56382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑