Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,073,492	A→G	W174R (TGG→CGG)	rutA ←	pyrimidine oxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,073,492	0	A	G	100.0%	73.1 / NA	21	W174R (TGG→CGG)	rutA	pyrimidine oxygenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/13);  total (8/13)

ACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCACAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATCTGCTCATACTCGGGCTTTTGCCAGCCAGTCACGAGGTT  >  NC_000913/1073357‑1073612                                                                                                                                        |                                                                                                                         aCCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAgg                                                                                                                      >  2:969840/1‑140 (MQ=255)      gCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTcgcg                                                                                                                 >  1:40908/1‑140 (MQ=255)         gagaACGCCATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCgcag                                                                                                              <  2:909040/140‑1 (MQ=255)            aaCGCCATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCAc                                                                                                           <  1:678907/140‑1 (MQ=255)                 cATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGaa                                                                                                      <  1:980698/140‑1 (MQ=255)                    gCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACat                                                                                                   >  1:561274/1‑140 (MQ=255)                     ccAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACata                                                                                                  <  1:651762/140‑1 (MQ=255)                              cTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTgag                                                                                         <  2:40908/140‑1 (MQ=255)                               tttGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTgaga                                                                                        <  2:387996/140‑1 (MQ=255)                                                  tttCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAacg                                                                             >  1:500242/1‑132 (MQ=255)                                                  tttCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAacg                                                                             <  2:500242/132‑1 (MQ=255)                                                    tCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGaaa                                                                   >  1:762418/1‑140 (MQ=255)                                                            acacTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATc                                                           >  1:711987/1‑140 (MQ=255)                                                               cTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCgcc                                                        <  2:762418/140‑1 (MQ=255)                                                                  ggTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCgccagg                                                     <  1:599313/140‑1 (MQ=255)                                                                    ttGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCgccaggcc                                                   <  2:711987/140‑1 (MQ=255)                                                                               cgcgACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATc                                        <  2:561274/140‑1 (MQ=255)                                                                                                 tGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATCTGCTCATACTCGGGCttt                      >  2:438016/1‑140 (MQ=255)                                                                                                        aTCGCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATCTGCTCATACTCGGGCTTTTgccagc               <  2:995297/140‑1 (MQ=255)                                                                                                           gCCTTTAAAATCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATCTGCTCATACTCGGGCTTTTGccagccag            >  2:408514/1‑140 (MQ=255)                                                                                                                     tCGCTTTTCCCCGTGCCCCGCAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATCTGCTCATACTCGGGCTTTTGCCAGCCAGTCACGAGGtt  <  1:438016/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                         ACCGGGCGGAGAACGCCATGCCAGCGTCGCTTTGCCCGGCGCAGATCACTTTCATGGGGACACTCGGTTGCGGACTCACGCGACAATCATTCATGGTGAAAAAATCGCCTTTAAAATCGCTTTTCCCCGTGCCCCACAGGTCGCGCAGCACCTGAACATATTCGGTGAGATAGTCGTAACGACGGGAGAAATAGTCATCGCCAGGCCAGATACCCATCTGCTCATACTCGGGCTTTTGCCAGCCAGTCACGAGGTT  >  NC_000913/1073357‑1073612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑