Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,137,693	C→T	S108F (TCC→TTC)	flgJ →	putative peptidoglycan hydrolase FlgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,137,693	0	C	T	100.0%	166.2 / NA	50	S108F (TCC→TTC)	flgJ	putative peptidoglycan hydrolase FlgJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/23);  total (27/23)

AGCACACTCGCCTGTATACCAGTATGTATGACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTCCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCC  >  NC_000913/1137564‑1137827                                                                                                                                  |                                                                                                                                       agtacacTCGCCTGTATACCAGTATGTATGACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCcagca                                                                                                                              >  2:334196/4‑140 (MQ=255) aGCACACTCGCCTGTATACCAGTATGTATGACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGGTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCcagca                                                                                                                              >  1:186518/1‑140 (MQ=255)                   ccAGTATGTATGACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCg                                                                                                            <  2:650101/140‑1 (MQ=255)                      gtatgtatGACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTc                                                                                                         >  1:233593/1‑140 (MQ=255)                        atgtatgACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGa                                                                                                       >  1:338625/1‑140 (MQ=255)                         tgtatgACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCT‑GGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGaaa                                                                                                     >  1:129262/1‑140 (MQ=255)                           tatgACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAAc                                                                                                    >  2:612597/1‑140 (MQ=255)                              gACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACtgt                                                                                                 >  1:418281/1‑140 (MQ=255)                              gACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACtgt                                                                                                 >  1:428635/1‑140 (MQ=255)                               aCCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACtgtg                                                                                                >  1:855527/1‑140 (MQ=255)                                   gcagATTGCCCAACAGATGACGGCGGGCAAAGGTCT‑GGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCg                                                                                           <  2:129262/140‑1 (MQ=255)                                   gcagATTGCCCAACAGATGACGGCGGGCAAAGGGCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGGGGTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGc                                                                                            >  1:450631/1‑140 (MQ=255)                                           cccAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGATTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCaa                                                                                    >  1:531652/1‑140 (MQ=255)                                                  gATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAgg                                                                             <  1:630284/140‑1 (MQ=255)                                                   aTGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCGTTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGgc                                                                            >  2:916838/1‑140 (MQ=255)                                                          cggGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCg                                                                     >  1:904190/1‑140 (MQ=255)                                                          cggGCAAAGGGCTGGGGCTTGCCGTGATGATTGATAAACAGAAGACGCCAGAACAACCATTGCCAGAGGGATTCACGCCACCAGCACCGATGAAATTCCCGCTCGAAACTGTGGGGCGTTATCAAAATCAGGCGCTTTCg                                                                     >  2:584282/1‑140 (MQ=255)                                                           gggCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGgcg                                                                           >  2:805494/1‑133 (MQ=255)                                                           gggCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGgcg                                                                           <  1:805494/133‑1 (MQ=255)                                                             gCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAg                                                                  >  2:206231/1‑140 (MQ=255)                                                              cAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGc                                                                 <  2:428635/140‑1 (MQ=255)                                                              cAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGc                                                                 <  2:418281/140‑1 (MQ=255)                                                                aaGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTg                                                               <  2:855527/140‑1 (MQ=255)                                                                             tGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAgg                                                     <  2:360821/137‑1 (MQ=255)                                                                             tGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAgg                                                     >  1:360821/1‑137 (MQ=255)                                                                             tGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCg                                                  >  2:811225/1‑140 (MQ=255)                                                                             tGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGC‑AAAGGCCGt                                                 >  2:136697/1‑140 (MQ=255)                                                                               cAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTg                                                <  1:811225/140‑1 (MQ=255)                                                                                  agaTGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCa                                             <  2:904190/140‑1 (MQ=255)                                                                                  agaTGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCa                                             <  1:334196/140‑1 (MQ=255)                                                                                  agaTGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCa                                             <  2:338625/140‑1 (MQ=255)                                                                                    atgatgGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACg                                           >  1:313522/1‑140 (MQ=255)                                                                                      gatgGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTa                                         >  2:409912/1‑140 (MQ=255)                                                                                         ggTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACt                                      <  2:517603/140‑1 (MQ=255)                                                                                                   aTGACGCCAGAACAACCATTGCCAGAGGATTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTc                            <  2:531652/140‑1 (MQ=255)                                                                                                    tGACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCg                           >  1:514637/1‑140 (MQ=255)                                                                                                     gACGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGc                          >  2:763956/1‑140 (MQ=255)                                                                                                      aCGCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCt                         >  2:742741/1‑140 (MQ=255)                                                                                                        gCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGc                       <  2:186518/140‑1 (MQ=255)                                                                                                        gCCAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGc                       <  1:916838/140‑1 (MQ=255)                                                                                                          cAGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCg                     >  1:466580/1‑140 (MQ=255)                                                                                                           aGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGAtt                             <  1:1014325/131‑1 (MQ=255)                                                                                                           aGAACAACCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGAtt                             >  2:1014325/1‑131 (MQ=255)                                                                                                             aacaacCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGt                  <  2:514637/140‑1 (MQ=255)                                                                                                               caacCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGa                <  2:233593/140‑1 (MQ=255)                                                                                                                 acCATTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACa              <  1:206231/140‑1 (MQ=255)                                                                                                                    aTTGCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTa           <  1:612597/140‑1 (MQ=255)                                                                                                                       gCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAg        >  2:228493/1‑140 (MQ=255)                                                                                                                       gCCAGAGGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAg        >  1:937372/1‑140 (MQ=255)                                                                                                                          agagGAGTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCAt     >  1:246496/1‑140 (MQ=255)                                                                                                                           gaggagTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGC‑AAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTc   <  1:136697/140‑1 (MQ=255)                                                                                                                            aggagTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTc   >  1:315886/1‑140 (MQ=255)                                                                                                                             ggagTTCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTcc  <  2:315886/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       AGCACACTCGCCTGTATACCAGTATGTATGACCAGCAGATTGCCCAACAGATGACGGCGGGCAAAGGTCTGGGGCTTGCAGAGATGATGGTTAAACAGATGACGCCAGAACAACCATTGCCAGAGGAGTCCACGCCAGCAGCACCGATGAAATTCCCGCTCGAAACTGTGGTGCGTTATCAAAATCAGGCGCTTTCGCAGCTGGTGCAAAAGGCCGTGCCACGTAACTACGATGATTCGCTGCCGGGTGACAGTAAAGCATTCC  >  NC_000913/1137564‑1137827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑