Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,324,319	A→G	E74E (GAA→GAG)	yciO →	putative RNA‑binding protein YciO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,324,319	0	A	G	100.0%	138.2 / NA	39	E74E (GAA→GAG)	yciO	putative RNA‑binding protein YciO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/20);  total (19/20)

TGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCAT  >  NC_000913/1324183‑1324453                                                                                                                                         |                                                                                                                                       tGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAACGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGctc                                                                                                                                     <  2:743154/140‑1 (MQ=255)   aTTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCtctc                                                                                                                                   <  2:73494/140‑1 (MQ=255)   aTTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCtctc                                                                                                                                   >  2:491484/1‑140 (MQ=255)   aTTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCtctc                                                                                                                                   <  1:958888/140‑1 (MQ=255)     tGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGa                                                                                                                                 >  1:338414/1‑140 (MQ=255)       tttATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGAcc                                                                                                                               <  1:310788/140‑1 (MQ=255)       tttATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGAcc                                                                                                                               <  2:243984/140‑1 (MQ=255)         tATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTa                                                                                                                             >  2:376328/1‑140 (MQ=255)         tATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTa                                                                                                                             >  1:19071/1‑140 (MQ=255)           tCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTAtt                                                                                                                           >  2:961427/1‑140 (MQ=255)           tCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCACCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTAtt                                                                                                                           <  2:696052/140‑1 (MQ=255)              aaCTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCAt                                                                                                                        <  2:19071/140‑1 (MQ=255)               aCTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTAtt                                                                                                                           <  2:280998/136‑1 (MQ=255)               aCTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTAtt                                                                                                                           >  1:280998/1‑136 (MQ=255)                cTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCAttt                                                                                                                      <  2:338414/140‑1 (MQ=255)                 tGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATttg                                                                                                                     >  2:882418/1‑140 (MQ=255)                   aTTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATttgtt                                                                                                                   >  2:288859/1‑140 (MQ=255)                       cGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATa                                                                                                               <  2:391447/140‑1 (MQ=255)                                 cTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGcgtt                                                                                                     <  1:491484/140‑1 (MQ=255)                                   cGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCACCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGcgtttc                                                                                                   >  2:1034033/1‑140 (MQ=255)                                    ggCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGcgtttcg                                                                                                  >  1:715119/1‑140 (MQ=255)                                                            gcgATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCggg                                                                          <  2:641176/140‑1 (MQ=255)                                                            gcgATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCggg                                                                          <  1:882418/140‑1 (MQ=255)                                                                       tATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGTGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCt                                                               <  2:1008742/140‑1 (MQ=255)                                                                       tATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCt                                                               <  2:522113/140‑1 (MQ=255)                                                                            gTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATc                                                          >  2:100830/1‑140 (MQ=255)                                                                                 aTTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGaa                                                     <  2:585619/140‑1 (MQ=255)                                                                                    cGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAggg                                                  <  2:715119/140‑1 (MQ=255)                                                                                    cGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAggg                                                  >  2:122868/1‑140 (MQ=255)                                                                                          cTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACgaa                                            >  2:248403/1‑140 (MQ=255)                                                                                               ggACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACgaaggaag                                       >  2:701833/1‑140 (MQ=255)                                                                                                      cacaACTTTACCCTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACg                                <  1:376328/140‑1 (MQ=255)                                                                                                                cccTGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCCGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTg                      <  2:596483/140‑1 (MQ=255)                                                                                                                   tGATGTGTCGCGATCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAg                   >  2:739770/1‑140 (MQ=255)                                                                                                                               aTCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCaaa       >  1:381187/1‑140 (MQ=255)                                                                                                                                tCTTTCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCaaaa      <  2:761019/140‑1 (MQ=255)                                                                                                                                  tttCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCaaaaac    >  2:10011/1‑138 (MQ=255)                                                                                                                                  tttCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAAcc    >  2:298247/1‑140 (MQ=255)                                                                                                                                    tCTGAGCTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCAt  >  2:148730/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TGATTGTTTATCCAACTGATTCCGGCTATGCGCTCGGCTGTAAAATTGAAGATAAAAACGCGATGGAGCGTATTTGTCGTATTCGCCAGCTGCCGGACGGTCACAACTTTACCCTGATGTGTCGCGATCTTTCTGAACTCTCGACCTATTCATTTGTTGATAACGTTGCGTTTCGTTTAATGAAAAACAACACGCCGGGCAACTATACCTTCATCCTGAAGGGGACGAAGGAAGTGCCACGCCGTCTGTTGCAGGAAAAACGCAAAACCAT  >  NC_000913/1324183‑1324453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑