Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,345,138	A→G	C535C (TGT→TGC)	pdeR ←	cyclic di‑GMP phosphodiesterase PdeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,345,138	0	A	G	100.0%	150.6 / NA	42	C535C (TGT→TGC)	pdeR	cyclic di‑GMP phosphodiesterase PdeR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/24);  total (18/24)

TGATGGCATCGATCGGAAAGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGAAATATTTACCGCCACTCGCAGGTTTATG  >  NC_000913/1345002‑1345273                                                                                                                                         |                                                                                                                                        tGATGGCATCGATCGGAAAGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCaa                                                                                                                                      <  1:247606/140‑1 (MQ=255)        atcgatcgGAAAGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTg                                                                                                                               >  1:541526/1‑140 (MQ=255)             tcgGAAAGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGt                                                                                                                          <  2:652742/140‑1 (MQ=255)             tcgGAAAGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGt                                                                                                                          <  1:617412/140‑1 (MQ=255)                  aaGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAAc                                                                                                                     <  1:172306/140‑1 (MQ=255)                  aaGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAAc                                                                                                                     <  1:894721/140‑1 (MQ=255)                  aaGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAAc                                                                                                                     <  1:520763/140‑1 (MQ=255)                    gcgcgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACAt                                                                                                                   <  2:541526/140‑1 (MQ=255)                    gcgcgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACAt                                                                                                                   <  1:835505/140‑1 (MQ=255)                       cgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAg                                                                                                                           >  2:938444/1‑129 (MQ=255)                       cgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAg                                                                                                                           <  1:938444/129‑1 (MQ=255)                       cgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGt                                                                                                                          <  2:912939/130‑1 (MQ=255)                       cgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGt                                                                                                                          >  1:912939/1‑130 (MQ=255)                       cgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCta                                                                                                                >  1:495393/1‑140 (MQ=255)                       cgcCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCta                                                                                                                >  2:620981/1‑140 (MQ=255)                            gTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAggg                                                                                                           <  2:182105/140‑1 (MQ=255)                            gTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAggg                                                                                                           <  1:353626/140‑1 (MQ=255)                              tGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCa                                                                                                         <  1:1026915/140‑1 (MQ=255)                                     gTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCa                                                                                                  <  1:36731/140‑1 (MQ=255)                                               gCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAAtt                                                                                             <  2:477772/135‑1 (MQ=255)                                               gCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAAtt                                                                                             >  1:477772/1‑135 (MQ=255)                                                  ggTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTGATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCt                                                                                     >  1:903530/1‑140 (MQ=255)                                                         aaaTCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAc                                                                              <  2:495393/140‑1 (MQ=255)                                                         aaaTCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAc                                                                              <  1:620981/140‑1 (MQ=255)                                                               tcCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAc                                                                              <  2:540967/134‑1 (MQ=255)                                                               tcCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAAc                                                                              >  1:540967/1‑134 (MQ=255)                                                                      gCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCg                                                                 <  1:989336/140‑1 (MQ=255)                                                                      gCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCg                                                                 <  2:627196/140‑1 (MQ=255)                                                                          ttGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGa                                                             >  1:419674/1‑140 (MQ=255)                                                                                  cTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAgg                                                                    >  2:27295/1‑125 (MQ=255)                                                                                  cTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAgg                                                                    <  1:27295/125‑1 (MQ=255)                                                                                            aaaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCGACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGa                                           >  2:618305/1‑140 (MQ=255)                                                                                                   tgAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGa                                      >  2:29361/1‑138 (MQ=255)                                                                                                   tgAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGa                                      <  1:29361/138‑1 (MQ=255)                                                                                                   tgAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACg                                    >  1:438022/1‑140 (MQ=255)                                                                                                          cagacagTGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGaa                             <  2:419674/140‑1 (MQ=255)                                                                                                                 tGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTg                                       <  1:755914/123‑1 (MQ=255)                                                                                                                 tGCCAGTTCATCATTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTg                                       >  2:755914/1‑123 (MQ=255)                                                                                                                           tcatTCTCAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGAAATATTTACCGCCACTCg            >  1:309039/1‑140 (MQ=255)                                                                                                                            ctttCTCAATCAGCCACCTCTCTGTCAGTTCAACATCTATGGGGCAGTATTCAAAATTGAGTTCCTGAAGACCCTGTTTCAGGGGGGTAAAAATGGTTTGATCGGGGAGCTGACGTGCAGAAATATTTACCGCCACTCGc           <  2:682214/138‑1 (MQ=255)                                                                                                                                 tcAATCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGAAATATTTACCGCCACTCGCAGGtt      >  1:682214/1‑140 (MQ=255)                                                                                                                                     tCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGAAATATTTACCGCCACTCGCAGGTTTATg  >  1:118469/1‑140 (MQ=255)                                                                                                                                     tCAGGCAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGAAATATTTACCGCCACTCGCAGGTTTATg  >  1:642564/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGATGGCATCGATCGGAAAGCGCGCCAGTTGCGAAAGTGAAGAGTAGCCGGTACCAAAATCATCCAGATGCACTTGCGCACCTAGTTGGCTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGATCGGCGAGCTGACGTGCAGAAATATTTACCGCCACTCGCAGGTTTATG  >  NC_000913/1345002‑1345273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑