Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	96,252	T→C	V84A (GTG→GCG)	mraY →	phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	96,252	0	T	C	100.0%	203.7 / NA	56	V84A (GTG→GCG)	mraY	phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/27);  total (29/27)

TGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGTGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACACCAAAG  >  NC_000913/96116‑96380                                                                                                                                         |                                                                                                                                 tgGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGAt                                                                                                                               <  1:173405/140‑1 (MQ=255) tgGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGAt                                                                                                                               >  2:687738/1‑140 (MQ=255)    aTGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGAtctc                                                                                                                            >  1:343792/1‑140 (MQ=255)    aTGGGCCCGCGTATGATTGCTCATTTGCAAAAACNTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGAtctc                                                                                                                            >  1:223018/1‑140 (MQ=255)           cgcgTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTActg                                                                                                                     >  2:254906/1‑140 (MQ=255)             cgTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTActgct                                                                                                                   >  1:196865/1‑140 (MQ=255)                  gATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTggg                                                                                                              <  1:889787/140‑1 (MQ=255)                      gCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTa                                                                                                          <  2:343792/140‑1 (MQ=255)                              gCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCa                                                                                                  <  2:449308/140‑1 (MQ=255)                                     cTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTa                                                                                           <  1:954331/140‑1 (MQ=255)                                       ttCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACg                                                                                         <  1:1010097/140‑1 (MQ=255)                                          cTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCt                                                                                      <  1:310398/140‑1 (MQ=255)                                                 cAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCgt                                                                               <  1:57501/140‑1 (MQ=255)                                                  aGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCgtg                                                                              >  2:337357/1‑140 (MQ=255)                                                         gCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTtggtgg                                                                       >  2:219886/1‑140 (MQ=255)                                                         gCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTtggtgg                                                                       >  2:655678/1‑140 (MQ=255)                                                         gCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTtggtg                                                                        <  1:598792/139‑1 (MQ=255)                                                         gCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTtggtg                                                                        >  2:598792/1‑139 (MQ=255)                                                         gCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTtggggg                                                                       >  2:437844/1‑140 (MQ=255)                                                                gacgGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGt                                                                <  1:219886/140‑1 (MQ=255)                                                                  cgGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAg                                                              >  1:470859/1‑140 (MQ=255)                                                                  cgGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAg                                                              >  1:880274/1‑140 (MQ=255)                                                                    gTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGt                                                            >  2:495359/1‑140 (MQ=255)                                                                    gTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGt                                                            >  1:103570/1‑140 (MQ=255)                                                                    gTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGt                                                            >  2:908833/1‑140 (MQ=255)                                                                        tGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACg                                                        <  2:470859/140‑1 (MQ=255)                                                                        tGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACg                                                        <  1:878090/140‑1 (MQ=255)                                                                        tGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACg                                                        <  1:254906/140‑1 (MQ=255)                                                                               cacTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTAt                                                 >  2:573884/1‑140 (MQ=255)                                                                               cacTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTAt                                                 >  2:151709/1‑140 (MQ=255)                                                                                 cTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGt                                                            <  1:507314/127‑1 (MQ=255)                                                                                 cTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGt                                                            >  2:507314/1‑127 (MQ=255)                                                                                  ttCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTgg                                              <  1:908833/140‑1 (MQ=255)                                                                                  ttCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTgg                                              <  2:273162/140‑1 (MQ=255)                                                                                    cAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCt                                            <  2:223018/140‑1 (MQ=255)                                                                                     aGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCtt                                           <  1:573884/140‑1 (MQ=255)                                                                                     aGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCtt                                           <  2:196865/140‑1 (MQ=255)                                                                                          gcgcGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTGCGGTGTTATTGGCTttgttg                                      >  1:583470/1‑140 (MQ=255)                                                                                          gcgcGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTttgttg                                      >  2:195462/1‑140 (MQ=255)                                                                                            gcgGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCt                                            >  2:645555/1‑132 (MQ=255)                                                                                            gcgGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCt                                            <  1:645555/132‑1 (MQ=255)                                                                                            gcgGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTtgat                                    >  1:562959/1‑140 (MQ=255)                                                                                               gTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTgatgat                                 <  1:485927/140‑1 (MQ=255)                                                                                               gTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTgatgat                                 <  2:880274/140‑1 (MQ=255)                                                                                                        ccATGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCGAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCaaa                        <  2:562959/140‑1 (MQ=255)                                                                                                          aTGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTGCGGTGTTATTGGCTTTGTTGATGATTATCGCAAAgt                      <  2:583470/140‑1 (MQ=255)                                                                                                          aTGGGCGGGATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAgt                      >  1:483229/1‑140 (MQ=255)                                                                                                                 ggATTATGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGt               >  1:240328/1‑140 (MQ=255)                                                                                                                       tGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTaa             <  1:313891/136‑1 (MQ=255)                                                                                                                       tGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTaa             >  2:313891/1‑136 (MQ=255)                                                                                                                       tGATCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGac         >  2:340877/1‑140 (MQ=255)                                                                                                                          tCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACAcc      <  2:483229/140‑1 (MQ=255)                                                                                                                          tCCTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACAcc      <  1:495359/140‑1 (MQ=255)                                                                                                                            cTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACACCaa    >  1:572170/1‑140 (MQ=255)                                                                                                                            cTGACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACACCaa    >  1:998328/1‑140 (MQ=255)                                                                                                                              gACGGCGATTGCGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACACCAAAg  >  2:368334/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                 TGGATGGGCCCGCGTATGATTGCTCATTTGCAAAAACTTTCCTTTGGTCAGGTGGTGCGTAACGACGGTCCTGAATCACACTTCAGCAAGCGCGGTACGCCGACCATGGGCGGGATTATGATCCTGACGGCGATTGTGATCTCCGTACTGCTGTGGGCTTACCCGTCCAATCCGTACGTCTGGTGCGTGTTGGTGGTGCTGGTAGGTTACGGTGTTATTGGCTTTGTTGATGATTATCGCAAAGTGGTGCGTAAAGACACCAAAG  >  NC_000913/96116‑96380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑