Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,373,436	C→T	S72L (TCG→TTG)	ycjO →	putative ABC transporter membrane subunit YcjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,373,436	0	C	T	100.0%	140.2 / NA	40	S72L (TCG→TTG)	ycjO	putative ABC transporter membrane subunit YcjO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/19);  total (21/19)

GGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCT  >  NC_000913/1373308‑1373567                                                                                                                                 |                                                                                                                                    ggcgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGa                                                                                                                          <  2:195264/140‑1 (MQ=255)   cgtggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACg                                                                                                                        <  2:255701/140‑1 (MQ=255)     tggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACg                                                                                                                        <  2:903703/138‑1 (MQ=255)     tggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACg                                                                                                                        <  2:675929/138‑1 (MQ=255)     tggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACg                                                                                                                        >  1:903703/1‑138 (MQ=255)     tggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACg                                                                                                                        >  1:675929/1‑138 (MQ=255)     tggcCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGt                                                                                                                      >  2:810700/1‑140 (MQ=255)                  cGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACcgcg                                                                                                         <  1:375789/140‑1 (MQ=255)                      tatCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCtgg                                                                                                     <  1:929122/140‑1 (MQ=255)                                      ttACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCAc                                                                                     <  2:460294/140‑1 (MQ=255)                                               ccGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCgg                                                                            >  1:490750/1‑140 (MQ=255)                                                                 gAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGGGATGtt                                                          >  2:779938/1‑140 (MQ=255)                                                                  aGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTTGCGATGttt                                                         >  1:198552/1‑140 (MQ=255)                                                                  aGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGttt                                                         >  2:666616/1‑140 (MQ=255)                                                                  aGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGttt                                                         >  2:885647/1‑140 (MQ=255)                                                                  aGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGGGGCGATGttt                                                         >  2:759524/1‑140 (MQ=255)                                                                             gttgGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGa                                              >  2:251950/1‑140 (MQ=255)                                                                                      aGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCt                                     >  2:323197/1‑140 (MQ=255)                                                                                                  cGTATCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACcgc                         >  1:255737/1‑140 (MQ=255)                                                                                                     aTCCTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACcgcgcg                      <  2:490750/140‑1 (MQ=255)                                                                                                       ccTCTCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGtt                    <  1:809514/140‑1 (MQ=255)                                                                                                        ctctCCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTc                   <  1:810700/140‑1 (MQ=255)                                                                                                          ctcCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCg                  >  1:596731/1‑139 (MQ=255)                                                                                                          ctcCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCg                  <  2:596731/139‑1 (MQ=255)                                                                                                          ctcCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGc                 >  1:945719/1‑140 (MQ=255)                                                                                                          ctcCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGc                 >  1:559068/1‑140 (MQ=255)                                                                                                          ctcCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGc                 >  2:54063/1‑140 (MQ=255)                                                                                                          ctcCGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGGGGCGGTGTTTTTCAACCGCGGGTTCCCCCTGCGCAAAACCGCGCGTTCGc                 >  2:507794/1‑140 (MQ=255)                                                                                                             cGATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTTGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTgg              <  2:198552/140‑1 (MQ=255)                                                                                                              gATCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGt             <  1:759524/140‑1 (MQ=255)                                                                                                                tCCCGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCTCCTGCGCAAAACCGCGCGTTCGCTGGTGa           >  2:113586/1‑140 (MQ=255)                                                                                                                  ccGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCTCCTGCGCAAAACCGCGCGTTCGCTGGTGATc         <  1:113586/140‑1 (MQ=255)                                                                                                                  ccGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATc         <  2:858737/140‑1 (MQ=255)                                                                                                                  ccGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATc         <  2:559068/140‑1 (MQ=255)                                                                                                                  ccGGCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATc         <  1:251950/140‑1 (MQ=255)                                                                                                                     gCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGAACctc      >  1:504550/1‑140 (MQ=255)                                                                                                                     gCTTCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCACCTGCGCAAAAACGCGCGTTCGCTGGTGATCctc      >  1:1041314/1‑140 (MQ=255)                                                                                                                       ttCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCtctc    <  2:298572/140‑1 (MQ=255)                                                                                                                       ttCTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCtctc    <  1:779938/140‑1 (MQ=255)                                                                                                                         cTGGCATTTGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCt  >  1:728215/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                    GGCGTGGCCGATGGTGTCGAATATCGAAATCAGTTTTTTACGTCTGCCGCTCAATCCCAACATCGAGTCAACGTTTGTTGGGGTGAGCAACTATGTGCGTATCCTCTCCGATCCCGGCTTCTGGCATTCGCTGTGGATGACGGTCTGGTATACCGCGCTGGTGGTGGCGGGCAGCACCGTGCTCGGGCTGGCGGTGGCGATGTTTTTCAACCGCGAGTTCCGCCTGCGCAAAACCGCGCGTTCGCTGGTGATCCTCTCCT  >  NC_000913/1373308‑1373567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑