Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,020	C→T	W61* (TGG→TGA)	pqqL ←	putative zinc peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,020	0	C	T	100.0%	178.4 / NA	50	W61* (TGG→TGA)	pqqL	putative zinc peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/29);  total (21/29)

GCCCATTGACTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGT  >  NC_000913/1574885‑1575154                                                                                                                                        |                                                                                                                                       gCCCATTGACTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATa                                                                                                                                    >  1:123941/1‑140 (MQ=255)    cATTGACTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAAt                                                                                                                                 >  1:851114/1‑140 (MQ=255)         aCTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTAcc                                                                                                                            <  1:204891/140‑1 (MQ=255)         aCTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTAcc                                                                                                                            <  2:400568/140‑1 (MQ=255)          cTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCt                                                                                                                           <  2:851114/140‑1 (MQ=255)          cTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCt                                                                                                                           <  1:570737/140‑1 (MQ=255)           tCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTg                                                                                                                          <  2:466018/140‑1 (MQ=255)           tCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTg                                                                                                                          >  2:45362/1‑140 (MQ=255)           tCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTg                                                                                                                          <  1:58222/140‑1 (MQ=255)             aaaTGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGAt                                                                                                                        >  2:497953/1‑140 (MQ=255)              aaTGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATc                                                                                                                       <  2:123941/140‑1 (MQ=255)                     cGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATGATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAAGTTACCTGATCCTTTGGa                                                                                                                >  2:928502/1‑140 (MQ=255)                            tttATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATCGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGa                                                                                                                         <  1:11723/124‑1 (MQ=255)                            tttATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATCGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGa                                                                                                                         >  2:11723/1‑124 (MQ=255)                             ttattaCCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATg                                                                                                        <  2:839750/140‑1 (MQ=255)                                  aCCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGAt                                                                                                   <  2:856652/140‑1 (MQ=255)                                  aCCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGAt                                                                                                   <  1:697918/140‑1 (MQ=255)                                      ggCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAAt                                                                                               <  2:760043/140‑1 (MQ=255)                                          aTGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCata                                                                                           <  2:158432/140‑1 (MQ=255)                                                  gtgCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCaa                                                                                   >  2:617326/1‑140 (MQ=255)                                                   tgCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAg                                                                                  <  2:480304/140‑1 (MQ=255)                                                   tgCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAg                                                                                  <  1:45362/140‑1 (MQ=255)                                                   tgCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAg                                                                                  <  1:981981/140‑1 (MQ=255)                                                      cGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCa                                                                               <  2:699682/140‑1 (MQ=255)                                                        ttAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTACTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCAtt                                                                             >  1:827315/1‑140 (MQ=255)                                                                   atGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGcc                                                                  <  2:526675/140‑1 (MQ=255)                                                                        cTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAg                                                                       <  1:787617/130‑1 (MQ=255)                                                                        cTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAg                                                                       >  2:787617/1‑130 (MQ=255)                                                                        cTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTa                                                             >  2:587491/1‑140 (MQ=255)                                                                        cTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTa                                                             >  1:515280/1‑140 (MQ=255)                                                                         tACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTaa                                                            >  1:535126/1‑140 (MQ=255)                                                                                tGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACtt                                                     >  1:226102/1‑140 (MQ=255)                                                                                tGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACtt                                                     >  1:649482/1‑140 (MQ=255)                                                                                   gCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTc                                                  <  2:649482/140‑1 (MQ=255)                                                                                    ccACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCa                                                 <  1:617326/140‑1 (MQ=255)                                                                                     cACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCAt                                                <  1:431285/140‑1 (MQ=255)                                                                                        gCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCt                                             >  2:481903/1‑140 (MQ=255)                                                                                        gCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCt                                             >  1:699198/1‑140 (MQ=255)                                                                                                cATTGTCTTCTTCCTGCAACGAACCGGTATGAATTTGCAGTCA‑AAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAgg                                    <  1:198580/140‑1 (MQ=255)                                                                                                    gTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAggcgg                                 >  1:303334/1‑140 (MQ=255)                                                                                                                    gAACCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGccc                 >  1:552493/1‑140 (MQ=255)                                                                                                                     aaCCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGTCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCg                <  2:535126/140‑1 (MQ=255)                                                                                                                     aaCCGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCg                <  1:587491/140‑1 (MQ=255)                                                                                                                        cGGTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCgggg             <  1:481903/140‑1 (MQ=255)                                                                                                                          gTATGAATTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAg           <  1:928502/140‑1 (MQ=255)                                                                                                                               aaTTTGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGTGGCAATCAGCCGCCCGGGGAGCAACa      <  2:303334/140‑1 (MQ=255)                                                                                                                                  ttGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACagag   >  2:152224/1‑140 (MQ=255)                                                                                                                                   tGCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGTAATCAGCCGCCCGGGGAGCAACAGAGt  >  2:667561/1‑140 (MQ=255)                                                                                                                                    gCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCaa        <  2:280805/133‑1 (MQ=255)                                                                                                                                    gCAGTCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCaa        >  1:280805/1‑133 (MQ=255)                                                                                                                                        |                                                                                                                                       GCCCATTGACTCAAATGTTTCGATGACTTTATTACCCGGCCATGTTTTTGTGCCGTTAAACATCATATGTTCTACAAAATGAGCCACGCCGAGCTCATTGTCTTCTTCCTGCAATGAACCGGTATGAATTTGCAGCCATAAATTTACCTGATCCTTTGGATGAGCATGCGGATAAATCATATATCGCAAGCCATTGTCCAGTTGCCCGGTAATTAACTTTTCATCCTGCGGTAAGGCGGCGGCAATCAGCCGCCCGGGGAGCAACAGAGT  >  NC_000913/1574885‑1575154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑