Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	118,198	A→G	L150L (TTG→CTG)	nadC ←	quinolinate phosphoribosyltransferase (decarboxylating)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	118,198	0	A	G	100.0%	172.3 / NA	48	L150L (TTG→CTG)	nadC	quinolinate phosphoribosyltransferase (decarboxylating)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/26);  total (22/26)

CCGGAGGCAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGT  >  NC_000913/118065‑118328                                                                                                                                      |                                                                                                                                   ccGGAGGCAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTg                                                                                                                              <  1:373313/140‑1 (MQ=255)  cGGAGGCAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGc                                                                                                                             <  2:461255/140‑1 (MQ=255)        cAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCcagca                                                                                                                                  >  1:982826/1‑129 (MQ=255)         aataatATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTgg                                                                                                                      <  1:256313/140‑1 (MQ=255)         aataatATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTgg                                                                                                                      >  1:981766/1‑140 (MQ=255)                   ttttCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAg                                                                                                            <  2:981766/140‑1 (MQ=255)                     ttCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCa                                                                                                          >  2:404524/1‑140 (MQ=255)                        tttGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAAtt                                                                                                       <  1:964665/140‑1 (MQ=255)                             tCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACa                                                                                                  <  1:296858/140‑1 (MQ=255)                             tCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACa                                                                                                  <  2:35318/140‑1 (MQ=255)                                 gAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGt                                                                                              >  1:35768/1‑140 (MQ=255)                                      cATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAAGGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGt                                                                                         >  1:912694/1‑140 (MQ=255)                                      cATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGt                                                                                         >  2:986327/1‑140 (MQ=255)                                      cATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGt                                                                                         >  2:181164/1‑140 (MQ=255)                                      cATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGt                                                                                         >  2:1028293/1‑140 (MQ=255)                                      cATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGt                                                                                         >  1:941593/1‑140 (MQ=255)                                           gAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGc                                                                                           >  1:912143/1‑133 (MQ=255)                                           gAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGc                                                                                           <  2:912143/133‑1 (MQ=255)                                           gAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCtt                                                                                    >  2:668253/1‑140 (MQ=255)                                           gAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCtt                                                                                    >  2:353080/1‑140 (MQ=255)                                           gAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCtt                                                                                    >  2:525861/1‑140 (MQ=255)                                            aaaGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTa                                                                                   <  1:404524/140‑1 (MQ=255)                                                ccccAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTgg                                                                               >  2:463504/1‑140 (MQ=255)                                                 cccAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGc                                                                              <  1:930633/140‑1 (MQ=255)                                                  ccAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCa                                                                             <  1:31780/140‑1 (MQ=255)                                                   cAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCaa                                                                            >  2:299952/1‑140 (MQ=255)                                                    aGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAAc                                                                           <  1:985957/140‑1 (MQ=255)                                                    aGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAAc                                                                           <  1:897988/140‑1 (MQ=255)                                                    aGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAAc                                                                           <  2:878582/140‑1 (MQ=255)                                                      aCGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTc                                                                         >  2:542903/1‑140 (MQ=255)                                                          tGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGa                                                                     >  1:246131/1‑140 (MQ=255)                                                                     gccgccgcAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGca                                                          <  2:941593/140‑1 (MQ=255)                                                                       cgccgcAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGcaca                                                        <  1:668253/140‑1 (MQ=255)                                                                                   ccGCGTATTTCAGAGCTGAACCCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCa                                            <  1:44088/140‑1 (MQ=255)                                                                                         aTTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGt                                      >  2:810414/1‑140 (MQ=255)                                                                                          tttCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGtt                                     <  2:35768/140‑1 (MQ=255)                                                                                          tttCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGtt                                     <  1:542903/140‑1 (MQ=255)                                                                                           ttCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTc                                    >  1:73092/1‑140 (MQ=255)                                                                                            tCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCg                                   <  1:181164/140‑1 (MQ=255)                                                                                            tCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCg                                   <  1:353080/140‑1 (MQ=255)                                                                                            tCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCg                                   <  1:463504/140‑1 (MQ=255)                                                                                                     aaCGCAGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAAc                          >  2:685245/1‑140 (MQ=255)                                                                                                          aGGCCGGGTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCAc                     <  2:431058/140‑1 (MQ=255)                                                                                                                ggTAAGGTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCG‑CCGTTAACAGCACGCGGGAt              <  1:525861/140‑1 (MQ=255)                                                                                                                      gTTTTGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGcc         >  2:950100/1‑140 (MQ=255)                                                                                                                          tGCGCGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCa     >  1:879864/1‑140 (MQ=255)                                                                                                                           gcgcGTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCaa    >  1:72145/1‑140 (MQ=255)                                                                                                                            cgcgTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAg   <  2:619835/140‑1 (MQ=255)                                                                                                                             gcgTATCCAGCAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGt  <  1:299952/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   CCGGAGGCAATAATATGGTTTTCTTTGATCAGGAAGGCATCAGAAAGCCCCAGACGGTGATTCGCTCCGCCGCCGCAAAGTACCGCGTATTTCAGAGCTGAACGCAGGCCGGGTAAGGTTTTGCGCGTATCCAACAACTGCGTGTTGGTGCCTTCCAGCAATTCGACATAGTGGCGTACCTTACTGGCAACTCCTGAAAGGGTTTGCACAAAATTAAGCGCAGTGCGTTCGCCCGTTAACAGCACGCGGGATGGGCCTTCAAGT  >  NC_000913/118065‑118328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑