Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,679,855	T→C	V100A (GTC→GCC)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,679,855	0	T	C	100.0%	166.0 / NA	46	V100A (GTC→GCC)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/21);  total (25/21)

TTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTGGATTGTTCAC  >  NC_000913/1679720‑1679982                                                                                                                                        |                                                                                                                                ttGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTcc                                                                                                                             >  1:947790/1‑140 (MQ=255)       tGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAACGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTg                                                                                                                       >  2:879889/1‑140 (MQ=255)          tGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTg                                                                                                                    <  2:947790/140‑1 (MQ=255)          tGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTg                                                                                                                    <  2:645725/140‑1 (MQ=255)              ccTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCg                                                                                                                >  1:268852/1‑140 (MQ=255)                   cgcgCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGttttt                                                                                                           >  2:401420/1‑140 (MQ=255)                      gcATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTcc                                                                                                        >  2:719637/1‑140 (MQ=255)                      gcATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCACCAACGCCTCCTATCTGGTGATCGTTTTTTcc                                                                                                        >  1:575214/1‑140 (MQ=255)                              cccGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAg                                                                                                >  2:531286/1‑140 (MQ=255)                                    cTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCttttt                                                                                          >  1:982516/1‑140 (MQ=255)                                          ggcggTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGa                                                                                    <  1:829330/140‑1 (MQ=255)                                                         tATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTgcg                                                                     >  1:293509/1‑140 (MQ=255)                                                          aTGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTgcgc                                                                    <  1:531286/140‑1 (MQ=255)                                                           tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGcc                                                                   >  1:517571/1‑140 (MQ=255)                                                           tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGcc                                                                   >  2:95688/1‑140 (MQ=255)                                                           tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGcc                                                                   >  1:663086/1‑140 (MQ=255)                                                           tGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGcc                                                                   >  2:390705/1‑140 (MQ=255)                                                                 ctaaGGGGTTGGCGTGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTg                                                             >  2:261825/3‑140 (MQ=255)                                                                 cgAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTg                                                             >  1:396115/1‑140 (MQ=255)                                                                  gAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTgcgc                                                                    <  2:514222/132‑1 (MQ=255)                                                                  gAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTgcgc                                                                    >  1:514222/1‑132 (MQ=255)                                                                  gAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTgg                                                            >  1:225896/1‑140 (MQ=255)                                                                   aaGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGc                                                           <  1:401420/140‑1 (MQ=255)                                                                   aaGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGc                                                           <  2:225896/140‑1 (MQ=255)                                                                    aGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCg                                                          <  1:183135/140‑1 (MQ=255)                                                                    aGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCg                                                          <  1:95688/140‑1 (MQ=255)                                                                           ggCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTaa                                                   <  2:293509/140‑1 (MQ=255)                                                                                 cTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTg                                             >  1:12715/1‑140 (MQ=255)                                                                                  tAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATGCCTgg                                            <  1:879889/140‑1 (MQ=255)                                                                                              gTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTc                                >  2:876661/1‑140 (MQ=255)                                                                                                     aTGGGGATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCAt                         <  1:727661/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAGCGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  1:261825/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  1:876661/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  2:510212/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  2:517571/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  1:409805/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  1:390705/140‑1 (MQ=255)                                                                                                          gATACTGGCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  2:663086/140‑1 (MQ=255)                                                                                                                 gCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTa                 >  2:373120/1‑136 (MQ=255)                                                                                                                 gCTGTGCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTa                 <  1:373120/136‑1 (MQ=255)                                                                                                                   tgtgCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    >  1:1019855/1‑131 (MQ=255)                                                                                                                   tgtgCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCt                    <  2:1019855/131‑1 (MQ=255)                                                                                                                   tgtgCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTgg           >  1:435079/1‑140 (MQ=255)                                                                                                                   tgtgCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTgg           >  2:44093/1‑140 (MQ=255)                                                                                                                   tgtgCGCAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTgg           >  2:167795/1‑140 (MQ=255)                                                                                                                     tgCGCAGTCATCGCCAACGCCTCCTATCTGGCGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTGGAt         >  1:474998/1‑140 (MQ=255)                                                                                                                       cgcAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTGGATTg       >  1:725819/1‑140 (MQ=255)                                                                                                                         cAGTCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTGGATTGtt     <  2:890478/140‑1 (MQ=255)                                                                                                                            tCATCGCCAACGCCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTGGATTGTTCAc  >  1:170984/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                TTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATGCCCGCGAAGGGTTTGGCGAGCTAATCGGCTTTTGTTCCGCATGGGGATACTGGCTGTGCGCAGTCATCGCCAACGTCTCCTATCTGGTGATCGTTTTTTCCGCGTTAAGCTTTTTTACGGACACGCCGGAATTGCGCCTGTTTGGCGATGGTAATACCTGGCAATCGATAGTCGGTGCATCGGCTTTATTGTGGATTGTTCAC  >  NC_000913/1679720‑1679982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑