Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,725,250	T→C	H128R (CAC→CGC)	ydhF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,725,250	0	T	C	100.0%	163.8 / NA	46	H128R (CAC→CGC)	ydhF	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/23);  total (23/23)

GCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCG  >  NC_000913/1725116‑1725381                                                                                                                                       |                                                                                                                                    gCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGAtt                                                                                                                                <  1:970216/140‑1 (MQ=255)     aCGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACa                                                                                                                            <  2:263917/140‑1 (MQ=255)          aTTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCaaa                                                                                                                       >  2:257311/1‑140 (MQ=255)          aTTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCaaa                                                                                                                       >  2:304588/1‑140 (MQ=255)                    ggCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTTAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAgatgat                                                                                                             >  2:369233/1‑140 (MQ=255)                    ggCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAg                                                                                                                           <  1:634057/126‑1 (MQ=255)                    ggCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAg                                                                                                                           >  2:634057/1‑126 (MQ=255)                    ggCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAgatgat                                                                                                             >  1:779165/1‑140 (MQ=255)                    ggCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAgat                                                                                                                <  2:54532/137‑1 (MQ=255)                    ggCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAgat                                                                                                                >  1:54532/1‑137 (MQ=255)                          ttGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTcg                                                                                                       <  2:404857/140‑1 (MQ=255)                             cgcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTcg                                                                                                       >  1:976408/1‑137 (MQ=255)                             cgcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTcg                                                                                                       <  2:976408/137‑1 (MQ=255)                             cgcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCga                                                                                                    >  2:198347/1‑140 (MQ=255)                                                   ccAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgc                                                                              <  1:198347/140‑1 (MQ=255)                                                     aaaaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGgcgc                                                                            <  1:257311/140‑1 (MQ=255)                                                       aaTGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGctc                                                                          >  2:1036907/1‑140 (MQ=255)                                                              cACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatga                                                                   >  2:270848/1‑140 (MQ=255)                                                                  ttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatga                                                                   <  1:248340/136‑1 (MQ=255)                                                                  ttGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAatga                                                                   >  2:248340/1‑136 (MQ=255)                                                                    gCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGAt                                                             <  1:140044/140‑1 (MQ=255)                                                                     ccGCTCTGATGCAGATGTTTTAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATc                                                            <  1:369233/140‑1 (MQ=255)                                                                       gCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgc                                                          >  2:216937/1‑140 (MQ=255)                                                                        ctctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgcg                                                         <  2:945893/140‑1 (MQ=255)                                                                          ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt                                                       <  1:649860/140‑1 (MQ=255)                                                                          ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt                                                       <  1:216937/140‑1 (MQ=255)                                                                          ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt                                                       <  1:1036907/140‑1 (MQ=255)                                                                          ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt                                                       <  1:420776/140‑1 (MQ=255)                                                                          ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt                                                       <  1:270848/140‑1 (MQ=255)                                                                          ctGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGt                                                       <  1:304588/140‑1 (MQ=255)                                                                             aTGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgc                                                          >  1:425272/1‑134 (MQ=255)                                                                             aTGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATcgc                                                          <  2:425272/134‑1 (MQ=255)                                                                                     gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTaa                                            >  1:647842/1‑140 (MQ=255)                                                                                     gTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTaa                                            >  1:324766/1‑140 (MQ=255)                                                                                      tttGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAAt                                           <  2:619370/140‑1 (MQ=255)                                                                                       ttGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATg                                          >  1:548211/1‑140 (MQ=255)                                                                                              cgTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATg                                   <  2:647842/140‑1 (MQ=255)                                                                                               gTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGa                                  >  1:321075/1‑140 (MQ=255)                                                                                               gTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGa                                  >  2:1026856/1‑140 (MQ=255)                                                                                                tCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGAc                                 >  2:490656/1‑140 (MQ=255)                                                                                                              ggCATCCATTAACGGGTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCAcgcg                   <  2:324766/140‑1 (MQ=255)                                                                                                                           gggTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGAt      <  1:833634/140‑1 (MQ=255)                                                                                                                             gTCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCTGTCGTCGCGATAc    >  2:922732/1‑140 (MQ=255)                                                                                                                              tCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAcc   >  2:476710/1‑140 (MQ=255)                                                                                                                              tCTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTAATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAcc   >  2:36526/1‑140 (MQ=255)                                                                                                                               cTGGTCGGCGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCg  >  2:110693/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                    GCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTCCGCCACTTCATCGGCATCCATTAACGGGTCTGGTCGGTGGATTAACAGCAAATCCAGATGATCGGTCGCGAGATTAATTAGCGACTGTTCGGCGCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCG  >  NC_000913/1725116‑1725381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑