| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,769,479 | A→G | T136A (ACG→GCG) | ydiK → | putative transporter YdiK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,769,479 | 0 | A | G | 98.1% | 186.8 / ‑3.3 | 53 | coding (406/1113 nt) | ydiK | putative transporter YdiK |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (21/31); minor base . (1/0); total (22/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
TCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCG > NC_000913/1769342‑1769612 | tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCg > 1:868426/1‑140 (MQ=255)tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCg > 1:98512/1‑140 (MQ=255) aCGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGgcggcg > 2:80353/1‑140 (MQ=255) aCGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGgcggcg > 1:323237/1‑140 (MQ=255) ggCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGAt > 1:441647/1‑140 (MQ=255) ggCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGAt > 2:47680/1‑140 (MQ=255) cGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATgg > 1:141033/1‑140 (MQ=255) tAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTc < 1:348400/140‑1 (MQ=255) tAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTc < 2:323237/140‑1 (MQ=255) tAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGcc < 2:868426/140‑1 (MQ=255) aaaGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGccc < 2:450709/140‑1 (MQ=255) aaaGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGccc < 2:632425/140‑1 (MQ=255) aaaGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGccc < 1:762631/140‑1 (MQ=255) aaGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCt < 1:356034/140‑1 (MQ=255) ccATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTcc > 2:519256/1‑132 (MQ=255) ccATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTcc < 1:519256/132‑1 (MQ=255) ccATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTtat < 1:950933/140‑1 (MQ=255) ccAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGc < 2:378325/140‑1 (MQ=255) tGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGG‑CGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccac > 2:815449/1‑140 (MQ=255) ttACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGtt > 1:680239/1‑140 (MQ=255) tACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTTGCACCACCACCACCTGGTTc > 1:708845/1‑140 (MQ=255) aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCg > 2:1008537/1‑140 (MQ=255) aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTg < 1:865320/130‑1 (MQ=255) aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTg > 2:865320/1‑130 (MQ=255) aTCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTggg < 2:804944/140‑1 (MQ=255) tGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAg < 1:555502/140‑1 (MQ=255) cTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCa > 1:146788/1‑140 (MQ=255) aTTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcg < 2:6680/140‑1 (MQ=255) ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg > 1:613172/1‑136 (MQ=255) ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg < 2:613172/136‑1 (MQ=255) ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGgcgc > 2:295861/1‑140 (MQ=255) ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCCGGCGCAACGTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCCCCACCACCTGGGTCGTTGGGCAGGCGGCGCATATCGGgcac > 2:258528/1‑138 (MQ=255) tCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt < 2:680239/140‑1 (MQ=255) aTTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGt > 1:750450/1‑140 (MQ=255) aTTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGt > 1:234795/1‑140 (MQ=255) ggCGCGAAGCTGTTTGCAGGTTGGCCCACCTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCa < 2:874556/140‑1 (MQ=255) cgcgAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCAtt < 1:47680/140‑1 (MQ=255) aGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTgcg > 2:938164/1‑140 (MQ=255) cTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCt < 1:295861/140‑1 (MQ=255) gTATGTAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGa < 2:362623/140‑1 (MQ=255) aTGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATg < 2:441647/140‑1 (MQ=255) tGCAGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCGCCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCGTTATGGTGCATTGTGCGCTGATGc < 1:258528/140‑1 (MQ=255) aGGCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGTTGCTtc > 1:652193/1‑140 (MQ=255) gCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTgc < 2:163576/127‑1 (MQ=255) gCTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTgc > 1:163576/1‑127 (MQ=255) cTGGCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTctct < 2:141033/140‑1 (MQ=255) ggCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTc > 1:630929/1‑140 (MQ=255) gCACAACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCa < 1:1008537/140‑1 (MQ=255) caACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg < 2:750450/140‑1 (MQ=255) caACTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg < 2:234795/140‑1 (MQ=255) aaCTTGCTGGATATGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGc < 2:98512/140‑1 (MQ=255) tatGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGTAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACt > 2:59639/1‑140 (MQ=255) tGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGccc < 2:644354/129‑1 (MQ=255) tGGGGGGGGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGccc > 1:644354/1‑129 (MQ=255) ggggggGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGTAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGgcg < 1:59639/140‑1 (MQ=255) ggggggGCGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGgcg < 2:652193/140‑1 (MQ=255) | TCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTGCTGTACTGGCG > NC_000913/1769342‑1769612 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |