Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,821,724	G→A	intergenic (‑105/+194)	chbB ← / ← osmE	N,N'‑diacetylchitobiose‑specific PTS enzyme IIB component/osmotically‑inducible lipoprotein OsmE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,821,724	0	G	A	100.0%	178.8 / NA	50	intergenic (‑105/+194)	chbB/osmE	N,N'‑diacetylchitobiose‑specific PTS enzyme IIB component/osmotically‑inducible lipoprotein OsmE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/31);  total (19/31)

AGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGGCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAA  >  NC_000913/1821587‑1821848                                                                                                                                          |                                                                                                                             aGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACt                                                                                                                            >  2:712085/1‑140 (MQ=255)   aaCAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGa                                                                                                                          >  1:1038129/1‑140 (MQ=255)   aaCAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGa                                                                                                                          <  2:285603/140‑1 (MQ=255)      aaaaCAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAg                                                                                                                       <  1:898444/140‑1 (MQ=255)       aaaCAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAgg                                                                                                                      >  1:429944/1‑140 (MQ=255)        aaCAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGa                                                                                                                     <  1:867520/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTTATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  1:986983/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAg                                                                                                                       >  2:612560/1‑133 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAg                                                                                                                       <  2:372608/133‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAg                                                                                                                       >  1:372608/1‑133 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAg                                                                                                                       <  1:612560/133‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                >  1:1011164/1‑140 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  2:865953/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  2:564877/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  2:429944/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  2:1038129/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  1:114310/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  1:222550/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  1:496305/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  1:370324/140‑1 (MQ=255)             aTAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  1:264829/140‑1 (MQ=255)                      tttCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                >  1:180944/1‑131 (MQ=255)                      tttCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  2:180944/131‑1 (MQ=255)                       ttCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTTAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                <  2:96445/130‑1 (MQ=255)                       ttCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTTAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGtata                                                                                                                >  1:96445/1‑130 (MQ=255)                       ttCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGAc                                                                                                      <  2:1011164/140‑1 (MQ=255)                       ttCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGAGACTGAAAGGAGTATACGGTATAGAc                                                                                                      <  2:371674/140‑1 (MQ=255)                                aTAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAAt                                                                                             <  1:784592/140‑1 (MQ=255)                                    aaCTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTa                                                                                         <  2:342537/140‑1 (MQ=255)                                         cccTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCt                                                                                    >  2:976020/1‑140 (MQ=255)                                         cccTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCt                                                                                    >  2:555315/1‑140 (MQ=255)                                         cccTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCt                                                                                    >  2:299139/1‑140 (MQ=255)                                              aTCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGAc                                                                               <  1:832746/140‑1 (MQ=255)                                                                       acTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCt                                                      <  1:712085/140‑1 (MQ=255)                                                                         tCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCtgt                                                    <  1:1000119/140‑1 (MQ=255)                                                                         tCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCtgt                                                    >  2:84389/1‑140 (MQ=255)                                                                         tCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCtgt                                                    >  2:172518/1‑140 (MQ=255)                                                                                 ccTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGa                                            <  1:555315/140‑1 (MQ=255)                                                                                           cTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGAcc                                          <  2:583926/132‑1 (MQ=255)                                                                                           cTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGAcc                                          >  1:583926/1‑132 (MQ=255)                                                                                            ttCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTcaca                                 >  1:1021534/1‑140 (MQ=255)                                                                                                      tGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCaa                       >  2:100022/1‑140 (MQ=255)                                                                                                        ggTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAaat                     >  2:834678/1‑140 (MQ=255)                                                                                                        ggTAACTTGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAaat                     >  1:788816/1‑140 (MQ=255)                                                                                                               tGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTcgc              <  1:976020/140‑1 (MQ=255)                                                                                                               tGCGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTcgc              <  2:1021534/140‑1 (MQ=255)                                                                                                                 cGAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGa            <  2:788816/140‑1 (MQ=255)                                                                                                                  gAAACCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGaa           >  1:819565/1‑140 (MQ=255)                                                                                                                    aaCCAACATGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGa         <  1:184859/140‑1 (MQ=255)                                                                                                                           aTGACTTTTTTTGTGACTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTaa  <  2:423170/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                             AGAACAAAACAGATAAATGTGTTTCTTTTCCATAAAACTGCCCTCATCGACGATTATCTGTCAGCCAGACACTCCGCAAGCCTTAACCTGCTTCCATGCTCTGGGTAACTTGCGAAACCAACATGACTTTTTTTGTGGCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAA  >  NC_000913/1821587‑1821848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑