Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,823,865	T→C	C117C (TGT→TGC)	cho →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,823,865	0	T	C	100.0%	169.1 / NA	47	C117C (TGT→TGC)	cho	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/27);  total (20/27)

ATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCA  >  NC_000913/1823731‑1823979                                                                                                                                       |                                                                                                                   aTGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCt                                                                                                               <  2:506804/140‑1 (MQ=255)  tGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTg                                                                                                              <  2:449073/140‑1 (MQ=255)            aTCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATg                                                                                                    >  1:184041/1‑140 (MQ=255)             tCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTGTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGa                                                                                                   >  2:717478/1‑140 (MQ=255)             tCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGa                                                                                                   >  2:914445/1‑140 (MQ=255)             tCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGa                                                                                                   >  1:637048/1‑140 (MQ=255)                     gATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGcgcg                                                                                           <  1:225686/140‑1 (MQ=255)                     gATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGcgcg                                                                                           <  1:877284/140‑1 (MQ=255)                      aTCAGCGGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGt                                                                                          <  2:415384/140‑1 (MQ=255)                        cAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCg                                                                                        <  2:184041/140‑1 (MQ=255)                           cTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGAtg                                                                                     <  1:807677/140‑1 (MQ=255)                           cTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGAtg                                                                                     <  2:234478/140‑1 (MQ=255)                           cTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGAtg                                                                                     <  2:735779/140‑1 (MQ=255)                           cTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGAtg                                                                                     <  1:287355/140‑1 (MQ=255)                                tatGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATgtggtg                                                                                <  1:914445/140‑1 (MQ=255)                                        gccgGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCaa                                                                        >  1:588121/1‑140 (MQ=255)                                        gccgGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCaa                                                                        >  1:264854/1‑140 (MQ=255)                                         ccgGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCaaa                                                                       >  2:975218/1‑140 (MQ=255)                                         ccgGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCa                                                                         >  1:151614/1‑138 (MQ=255)                                         ccgGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCa                                                                         <  2:151614/138‑1 (MQ=255)                                            gTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAagag                                                                    >  1:234985/1‑140 (MQ=255)                                             tGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAgg                                                                   >  1:937058/1‑140 (MQ=255)                                              gAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGt                                                                  >  1:854882/1‑140 (MQ=255)                                                        ctctGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCa                                                        <  1:280716/140‑1 (MQ=255)                                                         tctGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCAc                                                       >  1:527948/1‑140 (MQ=255)                                                          ctGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACg                                                      <  2:937058/140‑1 (MQ=255)                                                          ctGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACg                                                      <  1:975218/140‑1 (MQ=255)                                                          ctGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACg                                                      <  2:588121/140‑1 (MQ=255)                                                              tCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGcc                                                  <  1:717478/140‑1 (MQ=255)                                                                cTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGcccc                                                <  2:637048/140‑1 (MQ=255)                                                                   gAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCaa                                             >  1:731390/1‑140 (MQ=255)                                                                              aaTCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGcc                                  <  2:854882/140‑1 (MQ=255)                                                                              aaTCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGcc                                  <  2:731390/140‑1 (MQ=255)                                                                                 cAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGt                               <  1:625329/140‑1 (MQ=255)                                                                                         aacaGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAAt                       <  2:116685/140‑1 (MQ=255)                                                                                              ccGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGgcg                  >  1:7245/1‑140 (MQ=255)                                                                                                  tGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCa              <  2:7245/140‑1 (MQ=255)                                                                                                        ataaACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGgcgcg                >  1:158125/1‑132 (MQ=255)                                                                                                        ataaACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGgcgcg                <  2:158125/132‑1 (MQ=255)                                                                                                         taaACGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGc       <  2:983741/140‑1 (MQ=255)                                                                                                            aCGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCaa     >  2:687409/1‑139 (MQ=255)                                                                                                            aCGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCaa     >  2:167937/1‑139 (MQ=255)                                                                                                            aCGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCaa     <  1:687409/139‑1 (MQ=255)                                                                                                            aCGGTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCaa     <  1:167937/139‑1 (MQ=255)                                                                                                              ggTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAg    >  1:913354/1‑138 (MQ=255)                                                                                                              ggTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCa  >  1:579542/1‑140 (MQ=255)                                                                                                              ggTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACCAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCa  >  1:807506/1‑140 (MQ=255)                                                                                                              ggTTGCGCCGCAATCGCCAGCTCTGCGCCCTGCAATTAAATGAAAAGCGCGTCGATCTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAg    <  2:913354/138‑1 (MQ=255)                                                                                                                                       |                                                                                                                   ATGCTACGGCAATCCCGACGGATCAGCTGGATATGTACCGCCGGTGAAATCGGCGCTCTGCTCCTTGAAGCGCGATTAATCAAAGAACAACAGCCGCTGTTTAATAAACGGTTGCGCCGCAATCGCCAGCTCTGTGCCCTGCAATTAAATGAAAAGCGCGTCGATGTGGTGTATGCCAAAGAGGTGGATTTTTCACGAGCCCCCAACCTGTTTGGCCTGTTTGCCAATAGGCGCGCAGCTTTGCAAGCA  >  NC_000913/1823731‑1823979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑