Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	125,718	G→A	P8P (CCG→CCA)	aceF →	pyruvate dehydrogenase, E2 subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	125,718	0	G	A	100.0%	179.2 / NA	50	P8P (CCG→CCA)	aceF	pyruvate dehydrogenase, E2 subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/26);  total (24/26)

GCGAAATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCG  >  NC_000913/125598‑125854                                                                                                                         |                                                                                                                                         gCGAAATCGATAAGAAAGTGGTTGTTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAg                                                                                                                       >  2:918901/1‑140 (MQ=255) gCGAAATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAg                                                                                                                       >  2:83643/1‑140 (MQ=255)      aTCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGaa                                                                                                                  <  1:366727/140‑1 (MQ=255)        cGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAAt                                                                                                                <  1:1018339/140‑1 (MQ=255)             aGAAAGTGGTTGTTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCg                                                                                                           <  1:918901/140‑1 (MQ=255)             aGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTCACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCg                                                                                                           <  2:910129/140‑1 (MQ=255)             aGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCg                                                                                                           <  2:612647/140‑1 (MQ=255)                  gTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATc                                                                                                      <  1:107985/140‑1 (MQ=255)                   tGGTTGCTGACGCAATCGCTAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATcc                                                                                                     >  1:340815/1‑140 (MQ=255)                   tGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATcc                                                                                                     >  1:555796/1‑140 (MQ=255)                         cTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCa                                                                                               >  1:107364/1‑140 (MQ=255)                           gACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCaaa                                                                                             >  1:781893/1‑140 (MQ=255)                                aaTCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTggg                                                                                        <  1:1002540/140‑1 (MQ=255)                                 aTCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGc                                                                                       >  1:260943/1‑140 (MQ=255)                                 aTCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGc                                                                                       >  1:222050/1‑140 (MQ=255)                                                    aTGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGt                                                                               >  2:1028746/1‑129 (MQ=255)                                                    aTGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGt                                                                               <  1:1028746/129‑1 (MQ=255)                                                    aTGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAc                                                                    <  1:609213/140‑1 (MQ=255)                                                    aTGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAc                                                                    <  2:180516/140‑1 (MQ=255)                                                     tGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACa                                                                   >  1:809912/1‑140 (MQ=255)                                                         gATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCg                                                               >  2:850361/1‑140 (MQ=255)                                                           tAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCt                                                             <  1:494307/140‑1 (MQ=255)                                                             aaGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGa                                                           <  2:222050/140‑1 (MQ=255)                                                                  aaCCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCAcc                                                      >  2:477842/1‑140 (MQ=255)                                                                    cccGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAc                                                                    >  1:55091/1‑124 (MQ=255)                                                                    cccGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAAc                                                                    <  2:55091/124‑1 (MQ=255)                                                                    cccGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGt                                                    >  2:613395/1‑140 (MQ=255)                                                                    cccGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGt                                                    >  1:303034/1‑140 (MQ=255)                                                                        cgTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGaa                                                >  2:136147/1‑140 (MQ=255)                                                                        cgTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGaa                                                >  2:296400/1‑140 (MQ=255)                                                                        cgTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGaa                                                >  1:462778/1‑140 (MQ=255)                                                                               cGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCATGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACa                                         <  2:279758/140‑1 (MQ=255)                                                                                   agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                     <  1:318050/140‑1 (MQ=255)                                                                                   agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                     <  1:613395/140‑1 (MQ=255)                                                                                   agagGTAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGc                                     >  2:541922/1‑140 (MQ=255)                                                                                        tAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTa                                >  1:1036116/1‑140 (MQ=255)                                                                                        tAAAAGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTa                                >  2:2614/1‑140 (MQ=255)                                                                                          aaaGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATg                              <  1:296400/140‑1 (MQ=255)                                                                                          aaaGAATAATGGCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATg                              <  1:850361/140‑1 (MQ=255)                                                                                                    ggCTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGt                    <  2:107364/140‑1 (MQ=255)                                                                                                      cTATCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGtct                  >  1:620580/1‑140 (MQ=255)                                                                                                        aTCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTcc                >  2:614318/1‑140 (MQ=255)                                                                                                        aTCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTcc                <  2:781893/140‑1 (MQ=255)                                                                                                         tCGAAATCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCg               <  1:136147/140‑1 (MQ=255)                                                                                                             aaTCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAgg           <  2:340815/140‑1 (MQ=255)                                                                                                              aTCAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGc          <  1:541922/140‑1 (MQ=255)                                                                                                                cAAAGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCgg        >  2:602688/1‑140 (MQ=255)                                                                                                                 aaaGTACCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCggg       <  1:614318/140‑1 (MQ=255)                                                                                                                   aGTACCAGACATCGGGGTTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTa     <  2:620580/140‑1 (MQ=255)                                                                                                                      aCCAGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCg  <  1:83643/140‑1 (MQ=255)                                                                                                                         |                                                                                                                                         GCGAAATCGATAAGAAAGTGGTTGCTGACGCAATCGCCAAATTCAACATCGATGCAGATAAAGTTAACCCGCGTCTGGCGTAAGAGGTAAAAGAATAATGGCTATCGAAATCAAAGTACCGGACATCGGGGCTGATGAAGTTGAAATCACCGAGATCCTGGTCAAAGTGGGCGACAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCG  >  NC_000913/125598‑125854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑