Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,060,362	T→C	T185A (ACG→GCG)	cbl ←	DNA‑binding transcriptional activator Cbl

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,060,362	0	T	C	100.0%	290.8 / NA	79	T185A (ACG→GCG)	cbl	DNA‑binding transcriptional activator Cbl
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/37);  total (42/37)

AATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCAATATCAGCTTCGCCA  >  NC_000913/2060225‑2060495                                                                                                                                          |                                                                                                                                      aaTGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTTATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCa                                                                                                                                     >  2:866862/1‑140 (MQ=255)     aCATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATgg                                                                                                                                 >  1:844847/1‑140 (MQ=255)      cATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGt                                                                                                                                <  1:977033/140‑1 (MQ=255)      cATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGt                                                                                                                                >  1:750209/1‑140 (MQ=255)       aTCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTg                                                                                                                               >  1:720639/1‑140 (MQ=255)                 tGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTTATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGt                                                                                                                     <  1:866862/140‑1 (MQ=255)                 tGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGt                                                                                                                     <  1:148600/140‑1 (MQ=255)                 tGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGt                                                                                                                     >  2:907417/1‑140 (MQ=255)                 tGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGt                                                                                                                     <  2:280871/140‑1 (MQ=255)                  gcgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTc                                                                                                                    >  1:135469/1‑140 (MQ=255)                  gcgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTc                                                                                                                    >  2:893233/1‑140 (MQ=255)                   cgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   >  2:143822/1‑140 (MQ=255)                   cgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   <  1:893233/140‑1 (MQ=255)                   cgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   >  2:208266/1‑140 (MQ=255)                   cgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   >  1:167893/1‑140 (MQ=255)                   cgcACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   <  2:167893/140‑1 (MQ=255)                        ttAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGga                                                                                                              >  1:327033/1‑140 (MQ=255)                         tAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGgat                                                                                                             >  1:515/1‑140 (MQ=255)                          aaTACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGgatg                                                                                                            <  2:308985/140‑1 (MQ=255)                               aaTATCTGCCAGTAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCgt                                                                                                       <  1:328345/140‑1 (MQ=255)                                atatCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTc                                                                                                                    <  2:706929/126‑1 (MQ=255)                                atatCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTc                                                                                                                    >  1:706929/1‑126 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   >  1:425696/1‑125 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   <  2:425696/125‑1 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   <  1:496047/125‑1 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCa                                                                                                                   >  2:496047/1‑125 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGga                                                                                                              >  2:729628/1‑130 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGga                                                                                                              <  1:729628/130‑1 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGtgtg                                                                                                    >  1:732536/1‑140 (MQ=255)                                  atCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGtgtg                                                                                                    >  1:472617/1‑140 (MQ=255)                                    cTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGa                                                                                                  <  2:844847/140‑1 (MQ=255)                                     tGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGaa                                                                                                 <  1:143822/140‑1 (MQ=255)                                     tGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGaa                                                                                                 <  1:840406/140‑1 (MQ=255)                                      gCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAAc                                                                                                >  2:88387/1‑140 (MQ=255)                                        cAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACaa                                                                                              <  2:9971/140‑1 (MQ=255)                                        cAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACaa                                                                                              <  2:94073/140‑1 (MQ=255)                                            aaaCCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCaa                                                                                          >  1:456704/1‑140 (MQ=255)                                                     cGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATCTGCGTCAAGGGATGATCGTGTGGaa                                                                                                 <  1:172715/124‑1 (MQ=255)                                                     cGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATCTGCGTCAAGGGATGATCGTGTGGaa                                                                                                 >  2:172715/1‑124 (MQ=255)                                                                     caATTCGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACggg                                                                 <  1:757713/140‑1 (MQ=255)                                                                     caATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCa                                                                     >  1:47513/1‑136 (MQ=255)                                                                     caATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCa                                                                     <  2:47513/136‑1 (MQ=255)                                                                       aTACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGaa                                                               >  2:853145/1‑140 (MQ=255)                                                                              aGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGcgacg                                                        >  1:909874/1‑140 (MQ=255)                                                                              aGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGcgacg                                                        >  1:713454/1‑140 (MQ=255)                                                                                  ccccGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCt                                                    >  1:477493/1‑140 (MQ=255)                                                                                  ccccGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCt                                                    >  2:852276/1‑140 (MQ=255)                                                                                  ccccGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCt                                                    >  1:850963/1‑140 (MQ=255)                                                                                   cccGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTg                                                   >  1:223791/1‑140 (MQ=255)                                                                                    ccGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGCGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTg                                                   <  2:807183/139‑1 (MQ=255)                                                                                    ccGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGCGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTg                                                   >  1:807183/1‑139 (MQ=255)                                                                                        aaTCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGt                                              <  1:907417/140‑1 (MQ=255)                                                                                        aaTCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGt                                              <  1:652471/140‑1 (MQ=255)                                                                                        aaTCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGt                                              <  2:720639/140‑1 (MQ=255)                                                                                        aaTCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGt                                              <  1:812893/140‑1 (MQ=255)                                                                                        aaTCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGt                                              <  2:477493/140‑1 (MQ=255)                                                                                             ccTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTa                                         >  1:979425/1‑140 (MQ=255)                                                                                                 tCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCa                                     >  1:130661/1‑140 (MQ=255)                                                                                                 tCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCa                                     >  2:866928/1‑140 (MQ=255)                                                                                                    gTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAc                                  >  2:670115/1‑140 (MQ=255)                                                                                                     tAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcg                                 <  1:670115/140‑1 (MQ=255)                                                                                                      aaGTGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgc                                >  1:25199/1‑140 (MQ=255)                                                                                                         tGATTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgctcg                             <  2:223791/140‑1 (MQ=255)                                                                                                           aTTAACGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgctcgct                           >  1:49254/1‑140 (MQ=255)                                                                                                               aCGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgctcg                             >  1:150715/1‑134 (MQ=255)                                                                                                               aCGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCg                       <  2:979425/140‑1 (MQ=255)                                                                                                               aCGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCg                       <  2:472617/140‑1 (MQ=255)                                                                                                               aCGGCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGCGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgctcg                             <  2:150715/134‑1 (MQ=255)                                                                                                                 ggCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGAt                     <  2:909874/140‑1 (MQ=255)                                                                                                                  gCCACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATa                    <  2:456704/140‑1 (MQ=255)                                                                                                                    cACTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATAcc                  >  1:318500/1‑140 (MQ=255)                                                                                                                      cTTCGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCaa                >  1:1000395/1‑140 (MQ=255)                                                                                                                         cGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgctcg                             >  2:896814/1‑124 (MQ=255)                                                                                                                         cGCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAAcgctcg                             <  1:896814/124‑1 (MQ=255)                                                                                                                          gCTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCAATATc            <  2:732536/140‑1 (MQ=255)                                                                                                                           cTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGAt                     <  1:972676/130‑1 (MQ=255)                                                                                                                           cTATTGATTCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGAt                     >  2:972676/1‑130 (MQ=255)                                                                                                                                  ttCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCAATATCAGCTTCGc    <  2:130661/140‑1 (MQ=255)                                                                                                                                   tCCAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCAATATCAGCTTCGcc   >  2:286612/1‑140 (MQ=255)                                                                                                                                    ccAGCGCCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCAATATCAGCTTCGCCa  <  1:208266/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      AATGACATCAGAATCCTGCGCACTTAATACAATATCTGCCAGCAAACCTTTGCGGGCAAATGCGTCATCAATACGTGAGCGCCCCGTAATCCCCTGTCGGTAAGTGATTAACGGCCACTTCGCTATTGATTCCAGCGTCAATGGTGAAATTTGCGTCAAGGGATGATCGTGTGGAACAAGCAAACTATGGTGCCAACGAAACCACGGGAAGGCGACGAGCTGCGGGTCATTACTCAAACGCTCGCTGGCGATACCAATATCAGCTTCGCCA  >  NC_000913/2060225‑2060495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑