Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,217,643	T→C	L305L (TTA→TTG)	yehY ←	glycine betaine ABC transporter membrane subunit YehY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,217,643	0	T	C	100.0%	109.7 / NA	31	L305L (TTA→TTG)	yehY	glycine betaine ABC transporter membrane subunit YehY
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/13);  total (18/13)

TGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCACCAGCG  >  NC_000913/2217507‑2217776                                                                                                                                         |                                                                                                                                      tGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAAc                                                                                                                                    >  2:626120/1‑140 (MQ=255)      ctgctTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAAc                                                                                                                               >  1:304216/1‑140 (MQ=255)      ctgctTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAAc                                                                                                                               >  1:830098/1‑140 (MQ=255)                 ccccTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAAc                                                                                                                    >  1:668351/1‑140 (MQ=255)                   ccTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGc                                                                                                                  >  1:532375/1‑140 (MQ=255)                                 gcACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCAt                                                                                                    >  1:986253/1‑140 (MQ=255)                                      aaacCCGCCTGCCCCGATTAACCCCCCATTCCCCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCa                                                                                               <  1:103571/140‑1 (MQ=255)                                             ccTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCATGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGctct                                                                                        >  2:131803/1‑140 (MQ=255)                                               ggcgcCGATCACCGCCGAAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTgg                                                                                      <  2:668351/139‑1 (MQ=255)                                                   ccGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCATGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCt                                                                                  <  1:131803/140‑1 (MQ=255)                                                                        gCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGAt                                                             <  2:986253/140‑1 (MQ=255)                                                                        gCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGAt                                                             <  2:304216/140‑1 (MQ=255)                                                                             aCCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGt                                                        >  2:485290/1‑140 (MQ=255)                                                                               cTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTc                                                      <  2:145445/140‑1 (MQ=255)                                                                                 aCAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCaa                                                    >  2:1002310/1‑140 (MQ=255)                                                                                    gTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAAcc                                                 <  1:626120/140‑1 (MQ=255)                                                                                       tGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGAc                                              >  1:700086/1‑140 (MQ=255)                                                                                       tGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGAc                                              >  2:431260/1‑140 (MQ=255)                                                                                         cACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTa                                            >  2:560140/1‑140 (MQ=255)                                                                                               caccacCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCAcgc                                      >  2:331628/1‑140 (MQ=255)                                                                                                 ccaccCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCAcgccg                                    <  2:532375/140‑1 (MQ=255)                                                                                                  caccCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCAcgccgc                                   <  2:389316/140‑1 (MQ=255)                                                                                                        cAGGCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCa                             >  1:526042/1‑140 (MQ=255)                                                                                                          ggCTGCGCAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGc                           >  1:710175/1‑140 (MQ=255)                                                                                                              gcgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgca                       <  1:770293/140‑1 (MQ=255)                                                                                                               cgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgcag                      >  2:587146/1‑140 (MQ=255)                                                                                                               cgcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgcag                      >  1:1024604/1‑140 (MQ=255)                                                                                                                gcAGAAATACCGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGgcagc                     <  2:1017564/140‑1 (MQ=255)                                                                                                                         ccGGTAACGCCAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATag            >  1:287365/1‑140 (MQ=255)                                                                                                                                   cAGCGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCACCAgcg  <  2:384451/140‑1 (MQ=255)                                                                                                                                      cGGCAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCa        <  2:671083/131‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGGCGCTGCTTAGCAGCCCCTGGAAAACCAGCGCACCAAAACCGCCTGCGCCGATTAACGCCGCAATCACCGCCATACCTACAGTTTGCACCATCACCACCCGCAGGCTGCGCAGAAATACCGGTAACGCCAGCGGTAACTGAACATGCAGGAAACGCTGCGCCCCGCTCATCCCCATCGCTCTGGCGCTTTCCAGCACATCGCGCGGGATCTGGTTCAAACCGACTACCACGCCGCGCACCAGCGGCAGCAAGGCATAGAGCACCAGCG  >  NC_000913/2217507‑2217776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑