Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,988	T→C	Q813Q (CAA→CAG)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,988	0	T	C	100.0%	222.4 / NA	61	Q813Q (CAA→CAG)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/33);  total (28/33)

CATTCAGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCTTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCA  >  NC_000913/152852‑153123                                                                                                                                         |                                                                                                                                        cATTCAGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGt                                                                                                                                      <  1:998770/140‑1 (MQ=255) cATTCAGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGt                                                                                                                                      <  1:480239/140‑1 (MQ=255) cATTCAGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGt                                                                                                                                      <  2:788891/140‑1 (MQ=255)      aGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCAcc                                                                                                                                 <  2:28327/140‑1 (MQ=255)      aGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCAcc                                                                                                                                 <  2:944869/140‑1 (MQ=255)          taataGATTGTGCTATTTTCTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGt                                                                                                                             <  2:1026871/140‑1 (MQ=255)           aataGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTc                                                                                                                            >  2:604350/1‑140 (MQ=255)           aataGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTc                                                                                                                            <  2:807812/140‑1 (MQ=255)                  tgtgCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACa                                                                                                                       >  2:6748/1‑138 (MQ=255)                  tgtgCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACa                                                                                                                       <  1:6748/138‑1 (MQ=255)                  tgtgCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACAtt                                                                                                                     >  1:858556/1‑140 (MQ=255)                  tgtgCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACAtt                                                                                                                     >  1:829875/1‑140 (MQ=255)                    tgCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTAc                                                                                                                   >  2:595392/1‑140 (MQ=255)                       tATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCaa                                                                                                                <  2:954596/140‑1 (MQ=255)                          tttttCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCAGCCTGTCCAACATTACCAATGa                                                                                                             >  2:949276/1‑140 (MQ=255)                            tttCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACa                                                                                                           <  1:102384/140‑1 (MQ=255)                            tttCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACa                                                                                                           <  2:829875/140‑1 (MQ=255)                            tttCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACa                                                                                                           <  1:219152/140‑1 (MQ=255)                            tttCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACa                                                                                                           <  2:858556/140‑1 (MQ=255)                              tCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACAtt                                                                                                         >  2:94784/1‑140 (MQ=255)                                 gCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGcc                                                                                                      <  2:821784/140‑1 (MQ=255)                                 gCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGcc                                                                                                      <  2:569760/140‑1 (MQ=255)                                 gCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGcc                                                                                                      >  1:619641/1‑140 (MQ=255)                                     cTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGc                                                                                                  <  2:277488/140‑1 (MQ=255)                                      tGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCt                                                                                                 <  1:595392/140‑1 (MQ=255)                                        ggCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCtca                                                                                               <  2:619641/140‑1 (MQ=255)                                            ttgttgATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTcatcat                                                                                           >  2:11977/1‑140 (MQ=255)                                            ttgttgATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTcatcat                                                                                           >  2:175474/1‑140 (MQ=255)                                            ttgttgATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTcatcat                                                                                           >  2:180202/1‑140 (MQ=255)                                              gttgATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATaa                                                                                         >  2:1033330/1‑140 (MQ=255)                                                tgATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAat                                                                                       <  1:175474/140‑1 (MQ=255)                                                       gcgcAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTgca                                                                                >  2:1023098/1‑140 (MQ=255)                                                           aaGACAACTTACGGGTTTACTTTGTTCTAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCaa                                                                            <  1:890917/140‑1 (MQ=255)                                                                aaCTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGa                                                                       <  2:104196/140‑1 (MQ=255)                                                                 aCTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGaa                                                                      <  2:82676/140‑1 (MQ=255)                                                                       gggTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATAtttt                                                                <  1:222229/140‑1 (MQ=255)                                                                            tACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCa                                                           >  1:96667/1‑140 (MQ=255)                                                                             aCTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCAt                                                          <  1:1033330/140‑1 (MQ=255)                                                                             aCTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCAGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCAt                                                          <  2:277794/140‑1 (MQ=255)                                                                                 tGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACt                                                      <  1:94784/140‑1 (MQ=255)                                                                                  gTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACtt                                                     >  2:26021/1‑140 (MQ=255)                                                                                   ttCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTc                                                    >  1:953598/1‑140 (MQ=255)                                                                                        gCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGtgtg                                               <  2:953598/140‑1 (MQ=255)                                                                                        gCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGtgtg                                               <  1:472424/140‑1 (MQ=255)                                                                                           aTTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATg                                            <  1:26021/140‑1 (MQ=255)                                                                                             ttAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGtt                                          >  1:251376/1‑140 (MQ=255)                                                                                                   cTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATg                                            >  1:123952/1‑132 (MQ=255)                                                                                                   cTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATg                                            <  2:123952/132‑1 (MQ=255)                                                                                                    tGATATTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATg                                   >  2:646411/1‑140 (MQ=255)                                                                                                      atatTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATg                                            <  2:815929/129‑1 (MQ=255)                                                                                                      atatTTCCCTGCTGCTCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATg                                            >  1:815929/1‑129 (MQ=255)                                                                                                                gctgctCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCt                       >  2:784763/1‑140 (MQ=255)                                                                                                                gctgctCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCt                       >  2:205949/1‑140 (MQ=255)                                                                                                                    ctCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCa                   >  2:674725/1‑140 (MQ=255)                                                                                                                    ctCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCa                   <  1:784763/140‑1 (MQ=255)                                                                                                                    ctCAATACCACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATAGCTGATTGCCCTTGCa                   >  2:327627/1‑140 (MQ=255)                                                                                                                           ccACGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTc            >  1:97766/1‑140 (MQ=255)                                                                                                                              cGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGTAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCaaa         >  2:948344/1‑140 (MQ=255)                                                                                                                              cGAACAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCaaa         <  2:251376/140‑1 (MQ=255)                                                                                                                                 aCAAATGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATc      >  1:919428/1‑140 (MQ=255)                                                                                                                                     aTGCCTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCa  >  1:99757/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CATTCAGAATAATAGATTGTGCTATTTTTTCTGCTTCTGGGCTTTGTTGATAATGCGCAAGACAACTTACGGGTTTACTTTGTTCGAGCCATTTAATGCTGATATTTCCCTGCTGCTCAATACCACGAACAAATGCTTGTCCACCCTGTCCAACATTACCAATGACATTGCCTTGCTCATCATAAATATCTGCAGCAAATGGAATATTTTTACCATCACTTCGTGTGATGTTCATAATGGCTGATTGCCCTTGCACGGTTTCAAAATCAGCA  >  NC_000913/152852‑153123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑