Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,225,733	T→C	intergenic (‑102/‑68)	yohC ← / → yohD	putative inner membrane protein/DedA family protein YohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,225,733	0	T	C	100.0%	255.1 / NA	70	intergenic (‑102/‑68)	yohC/yohD	putative inner membrane protein/DedA family protein YohD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/38);  total (32/38)

TCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCCGCGCTGGTGATCGGTAGCCTGGCGG  >  NC_000913/2225597‑2225864                                                                                                                                         |                                                                                                                                    tCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCt                                                                                                                                  >  1:344058/1‑140 (MQ=255)    ggATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCtttt                                                                                                                               >  2:283802/1‑140 (MQ=255)    ggATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCtttt                                                                                                                               >  1:256852/1‑140 (MQ=255)    ggATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCtttt                                                                                                                               >  1:687982/1‑140 (MQ=255)               cAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAAt                                                                                                                    <  2:1038696/140‑1 (MQ=255)               cAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAAt                                                                                                                    >  1:434660/1‑140 (MQ=255)                 gTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGa                                                                                                                  >  1:551087/1‑140 (MQ=255)                  tCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGAt                                                                                                                 >  2:213296/1‑140 (MQ=255)                   ccccAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGAtt                                                                                                                >  1:772389/1‑140 (MQ=255)                      cAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  2:1034503/140‑1 (MQ=255)                      cAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  2:986443/140‑1 (MQ=255)                      cAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  2:965945/140‑1 (MQ=255)                      cAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  2:862010/140‑1 (MQ=255)                      cAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  1:330531/140‑1 (MQ=255)                      cAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  1:283802/140‑1 (MQ=255)                       aGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcaca                                                                                                            <  2:434660/140‑1 (MQ=255)                       aGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcaca                                                                                                            <  2:234167/140‑1 (MQ=255)                       aGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcaca                                                                                                            <  1:929783/140‑1 (MQ=255)                         aCATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAgg                                                                                                          >  1:1040269/1‑140 (MQ=255)                          cATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAggg                                                                                                         <  1:70841/140‑1 (MQ=255)                            tGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGgtg                                                                                                       >  2:711493/1‑140 (MQ=255)                              gCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTa                                                                                                     >  2:173224/1‑140 (MQ=255)                              gCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTa                                                                                                     >  1:793146/1‑140 (MQ=255)                                   tACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             <  1:243978/127‑1 (MQ=255)                                   tACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTcac                                                                                                             >  2:243978/1‑127 (MQ=255)                                   tACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCtt                                                                                                >  2:579646/1‑140 (MQ=255)                                        cccccTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTgg                                                                                           >  2:384778/1‑140 (MQ=255)                                        cccccTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTgg                                                                                           >  1:445151/1‑140 (MQ=255)                                        cccccTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTgg                                                                                           >  1:1016608/1‑140 (MQ=255)                                        cccccTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATACTTACTgg                                                                                           >  2:280752/1‑140 (MQ=255)                                           ccTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCaa                                                                                        <  2:1016608/140‑1 (MQ=255)                                              tgttgtGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTgg                                                                                           <  1:209332/134‑1 (MQ=255)                                              tgttgtGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTgg                                                                                           >  2:209332/1‑134 (MQ=255)                                               gttgtGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCaa                                                                                    >  1:894209/1‑140 (MQ=255)                                                     aGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGgag                                                                              >  2:508589/1‑140 (MQ=255)                                                     aGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGgag                                                                              >  2:579504/1‑140 (MQ=255)                                                     aGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGgag                                                                              <  2:344058/140‑1 (MQ=255)                                                      gTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGgaga                                                                             >  2:369115/1‑140 (MQ=255)                                                       ttCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagac                                                                            >  2:742539/1‑140 (MQ=255)                                                       ttCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagac                                                                            >  1:32026/1‑140 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         >  1:545898/1‑140 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  2:894209/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  1:508589/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  2:32026/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  2:793146/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  2:551087/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  1:579504/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  1:579646/140‑1 (MQ=255)                                                          aTGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacaga                                                                         <  1:969622/140‑1 (MQ=255)                                                           tGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGagacagac                                                                        <  2:772389/140‑1 (MQ=255)                                                             aaCCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTg                                                                      <  2:687982/140‑1 (MQ=255)                                                              aCCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTgg                                                                     <  1:173224/140‑1 (MQ=255)                                                                 tGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCt                                                                  >  2:957050/1‑140 (MQ=255)                                                                  gACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTa                                                                 >  1:570144/1‑140 (MQ=255)                                                                  gACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTa                                                                 <  2:777028/140‑1 (MQ=255)                                                                      gTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGa                                                             <  2:445151/140‑1 (MQ=255)                                                                      gTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGa                                                             <  2:1040269/140‑1 (MQ=255)                                                                       ttCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGAt                                                            <  1:213296/140‑1 (MQ=255)                                                                       ttCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGAt                                                            <  2:570144/140‑1 (MQ=255)                                                                                 aGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATacac                                                  >  2:203938/1‑140 (MQ=255)                                                                                  gTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACt                                                 <  1:384778/140‑1 (MQ=255)                                                                                  gTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATACTTACTGGCAACCAAAGGGAGACAGACTGGTCTATGGATCTCAATACACt                                                 <  1:280752/140‑1 (MQ=255)                                                                                       cTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTAtct                                            >  1:830683/1‑140 (MQ=255)                                                                                         tgtgATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTca                                          <  2:830683/140‑1 (MQ=255)                                                                                                         tCGCCCATGTCATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCcg                          <  1:715921/140‑1 (MQ=255)                                                                                                         tCGCCCATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCcg                          <  1:742539/140‑1 (MQ=255)                                                                                                            cccATGTAATTTTCACTGTCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCCgcgc                       <  2:545898/140‑1 (MQ=255)                                                                                                                              tCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCCGCGCTGGTGATCGGTAGCCTgg     >  1:937701/1‑140 (MQ=255)                                                                                                                              tCTGATGAACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCCGCGCTGGTGATCGGTAGCCTgg     >  1:353028/1‑140 (MQ=255)                                                                                                                                 gatgaACGCCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCCGCGCTGGTGATCGGTAGCCTGGCgg  <  1:711493/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TCGGGATGGGAAAACAGTCCCCAGACATGGCTCATACCGCCCCCTTGTTGTGAGTTCATGAACCTGACAGTTCAAGTATAAGTCAGCTTGTGATTATTTTTTGTTCGCCCATGTAATTTTCACTGTCTGATGAACGTCCTTTTTCAGCGTGAATGATTCACAGGGTGTATGCTTACTGGCAACCAAAGGGAGACAGACTGGCCTATGGATCTCAATACACTTATCTCACAATATGGTTATGCCGCGCTGGTGATCGGTAGCCTGGCGG  >  NC_000913/2225597‑2225864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑