Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,052	G→A	A84A (GCG→GCA)	yohD →	DedA family protein YohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,052	0	G	A	100.0%	197.2 / NA	55	A84A (GCG→GCA)	yohD	DedA family protein YohD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/30);  total (25/30)

TAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTC  >  NC_000913/2225916‑2226185                                                                                                                                         |                                                                                                                                      tAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAg                                                                                                                                    >  1:808038/1‑140 (MQ=255)     gTTCCCGCTGGCGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAAc                                                                                                                                >  2:627934/1‑140 (MQ=255)        cccGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTa                                                                                                                             >  2:1022384/1‑140 (MQ=255)        cccGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTa                                                                                                                             >  1:12308/1‑140 (MQ=255)        cccGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTa                                                                                                                             >  2:129757/1‑140 (MQ=255)        cccGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTa                                                                                                                             >  2:556973/1‑140 (MQ=255)          cGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGcaca                                                                                                                                     >  2:33212/1‑130 (MQ=255)          cGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGcaca                                                                                                                                     <  1:33212/130‑1 (MQ=255)            cTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCa                                                                                                                         <  2:48707/140‑1 (MQ=255)              ggtggtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAAc                                                                                                                       >  1:145484/1‑140 (MQ=255)                  gtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCa                                                                                                                   <  1:556973/140‑1 (MQ=255)                  gtACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCa                                                                                                                   <  2:632760/140‑1 (MQ=255)                     cTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATcc                                                                                                                >  1:942089/1‑140 (MQ=255)                       ttCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGt                                                                                                              <  1:129757/140‑1 (MQ=255)                        tCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCTGTa                                                                                                             <  1:129764/140‑1 (MQ=255)                          tgtgGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATc                                                                                                           >  2:390210/1‑140 (MQ=255)                            tgGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTg                                                                                                         >  2:884446/1‑140 (MQ=255)                            tgGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTg                                                                                                         <  1:1022384/140‑1 (MQ=255)                              gcgcTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGtt                                                                                                       >  2:1024396/1‑140 (MQ=255)                                 cTTGGCGGCATTATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGt                                                                                                    >  2:851930/1‑140 (MQ=255)                                 cTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGt                                                                                                    >  2:57098/1‑140 (MQ=255)                                    ggcggcATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCAt                                                                                                 >  2:532136/1‑140 (MQ=255)                                       ggcATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTgg                                                                                              <  2:373732/140‑1 (MQ=255)                                         cATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTa                                                                                            <  2:942089/140‑1 (MQ=255)                                         cATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTa                                                                                            <  1:1024396/140‑1 (MQ=255)                                           ttaTTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTAcg                                                                                          <  1:851930/138‑1 (MQ=255)                                           tGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTAcg                                                                                          <  1:649515/140‑1 (MQ=255)                                           tGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTAcg                                                                                          <  1:57098/140‑1 (MQ=255)                                            gATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTAcgc                                                                                         <  1:884446/140‑1 (MQ=255)                                            gATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTAcgc                                                                                         <  1:532136/140‑1 (MQ=255)                                               tGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCt                                                                                      <  2:364714/140‑1 (MQ=255)                                                ggCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCtt                                                                                     >  2:793254/1‑140 (MQ=255)                                                 gCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCttt                                                                                    <  1:793254/140‑1 (MQ=255)                                                  cGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTa                                                                                            <  2:1027767/131‑1 (MQ=255)                                                  cGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTa                                                                                            >  1:1027767/1‑131 (MQ=255)                                                      cAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTtatgta                                                                               <  1:757649/140‑1 (MQ=255)                                                      cAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTtatgta                                                                               <  1:175823/140‑1 (MQ=255)                                                      cAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTtatgta                                                                               <  1:1009144/140‑1 (MQ=255)                                                        ggTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTtatgtatg                                                                             >  2:698663/1‑140 (MQ=255)                                                                   tgtgCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTg                                                                  >  1:760523/1‑140 (MQ=255)                                                                       cgggcggCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTg                                                              >  2:475087/1‑140 (MQ=255)                                                                       cgggcggCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTg                                                              >  1:598890/1‑140 (MQ=255)                                                                         ggcggcGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGc                                                            >  2:953064/1‑140 (MQ=255)                                                                          gcggcgGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGcc                                                           <  1:963128/140‑1 (MQ=255)                                                                          gcggcgGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGcc                                                           <  2:760523/140‑1 (MQ=255)                                                                          gcggcgGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGcc                                                           <  2:808038/140‑1 (MQ=255)                                                                           cggcggTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGccc                                                          >  2:496974/1‑140 (MQ=255)                                                                                    ggcggcAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGAt                                                 <  1:698663/140‑1 (MQ=255)                                                                                    ggcggcAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGAt                                                 <  2:894932/140‑1 (MQ=255)                                                                                                        tttCTCGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTgccgc                             >  2:464948/1‑140 (MQ=255)                                                                                                          tctcGAAACATCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGccgccg                           >  1:881772/1‑140 (MQ=255)                                                                                                                   aTCAGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCttt                  <  1:390210/140‑1 (MQ=255)                                                                                                                      aGGATAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTg               <  1:156472/140‑1 (MQ=255)                                                                                                                          tAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGc           <  2:598890/140‑1 (MQ=255)                                                                                                                          tAAAATTGAGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGc           <  1:496974/140‑1 (MQ=255)                                                                                                                                  aGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCg            <  2:616/131‑1 (MQ=255)                                                                                                                                  aGCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCg            >  1:616/1‑131 (MQ=255)                                                                                                                                   gCGGGCACAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATtc  >  1:1004622/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TAAAGTTCCCGCTGGTGGTACTTTCTGTGGCGCTTGGCGGCATGATTGGCGACCAGGTGCTCTATCTGTGCGGGCGGCGGTTTGGCGGCAAGCTGTTACGCCGTTTCTCGAAACATCAGGATAAAATTGAGCGGGCGCAGAAACTTATCCAACGCCATCCGTATCTGTTTGTCATTGGTACGCGCTTTATGTATGGCTTTCGGGTGATTGGCCCGACGCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTC  >  NC_000913/2225916‑2226185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑