Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,249,622	C→T	intergenic (‑4/‑95)	yeiE ← / → yeiH	putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,249,622	0	C	T	100.0%	69.3 / NA	21	intergenic (‑4/‑95)	yeiE/yeiH	putative LysR‑type transcriptional regulator YeiE/conserved inner membrane protein YeiH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/14);  total (7/14)

CTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACA  >  NC_000913/2249487‑2249742                                                                                                                                        |                                                                                                                         cTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCtt                                                                                                                      <  2:582761/140‑1 (MQ=255)   gCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTAc                                                                                                                    <  2:996019/140‑1 (MQ=255)   gCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTAc                                                                                                                    <  1:654442/140‑1 (MQ=255)           aGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTtata                                                                                                            <  2:104728/140‑1 (MQ=255)                 gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTa                                                                                                                     >  2:562810/1‑125 (MQ=255)                 gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTa                                                                                                                     <  1:562810/125‑1 (MQ=255)                 gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTAcc                                                                                                                   <  2:759925/127‑1 (MQ=255)                 gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTAcc                                                                                                                   >  1:759925/1‑127 (MQ=255)                 gtcAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGatt                                                                                                      <  1:221379/140‑1 (MQ=255)                                  tgctgATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAAt                                                                                     >  1:192659/1‑140 (MQ=255)                                   gctgATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATc                                                                                    >  1:1042610/1‑140 (MQ=255)                                                                        tggtTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGc                                               <  1:289943/140‑1 (MQ=255)                                                                               tCCACTTTTCAATACTTCTGCAAAAACTTCCAGCTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTa                                        <  2:486591/140‑1 (MQ=255)                                                                               tCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTa                                        <  2:341334/140‑1 (MQ=255)                                                                                   cTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAgg                                    <  1:473451/140‑1 (MQ=255)                                                                                         aaTACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAggg                              >  1:952606/1‑140 (MQ=255)                                                                                              ttCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATg                         >  2:362329/1‑140 (MQ=255)                                                                                                     aaaaCTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAata                  <  2:192659/140‑1 (MQ=255)                                                                                                     aaaaCTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAata                  <  1:362329/140‑1 (MQ=255)                                                                                                       aaCTTCCAACTGCCGGAGGGTGATGTGCATAGTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATc                >  1:523005/1‑140 (MQ=255)                                                                                                                     ggAGGGTGATGTGCATATTTGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCGGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAaaca  <  2:928463/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                         CTGCCCTTCCAGGTCGGTCAAGGCTGCGCTCACTGCTGATTGCGACAACGCCAGCATCACCGACGCCTGGGTGGTTGATCCACTTTTCAATACTTCTGCAAAAACTTCCAACTGCCGGAGGGTGATGTGCATAGTCGCTTACCACTTATAAAGATTAATTATAAATATATAATCAATTTTATTTTTAAACCAGTTAGTCGTAACCTTATGCCCGGTTAAAGGAGAGGGTTATGACGAATATCACCTTACAGAAACA  >  NC_000913/2249487‑2249742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑