Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,598	T→C	R433R (CGT→CGC)	yejF →	putative oligopeptide ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,598	0	T	C	100.0%	165.2 / NA	46	R433R (CGT→CGC)	yejF	putative oligopeptide ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/23);  total (23/23)

GGCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCT  >  NC_000913/2277461‑2277734                                                                                                                                          |                                                                                                                                         ggCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCAt                                                                                                                                        >  2:271483/1‑140 (MQ=255)            tCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCa                                                                                                                             >  2:629091/1‑140 (MQ=255)                 aGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCa                                                                                                                        <  2:796575/140‑1 (MQ=255)                  gCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCAt                                                                                                                       <  2:144493/140‑1 (MQ=255)                    cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                                     <  1:271483/140‑1 (MQ=255)                    cGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTa                                                                                                                     >  2:342946/1‑140 (MQ=255)                     gACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTaa                                                                                                                    <  2:266307/140‑1 (MQ=255)                            tCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTaa                                                                                                             >  2:821377/1‑140 (MQ=255)                               gCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGcc                                                                                                          <  2:986690/140‑1 (MQ=255)                                   cacaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                      <  2:153699/140‑1 (MQ=255)                                    acaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                      <  2:767569/139‑1 (MQ=255)                                    acaACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCg                                                                                                      >  1:767569/1‑139 (MQ=255)                                     caACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCt                                                                                                    <  2:548390/140‑1 (MQ=255)                                           gAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCAt                                                                                              <  1:342946/140‑1 (MQ=255)                                             acaacaAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATAc                                                                                            >  2:322066/1‑140 (MQ=255)                                                acaaGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTtg                                                                                         >  2:267929/1‑140 (MQ=255)                                                           ccGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAca                                                                              >  2:28599/1‑140 (MQ=255)                                                           ccGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAca                                                                              >  1:987905/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  1:1016427/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  1:27362/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  2:15608/1‑140 (MQ=255)                                                              tGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGAcatca                                                                           >  1:5573/1‑140 (MQ=255)                                                               gATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTC‑‑‑TGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCAc                                                                       >  2:1012045/1‑140 (MQ=255)                                                                            ggATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAAc                                                             >  1:635677/1‑140 (MQ=255)                                                                                tAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTa                                                         <  1:15608/140‑1 (MQ=255)                                                                                  gATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACa                                                       <  1:267929/140‑1 (MQ=255)                                                                                        gAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCa                                                 >  1:750056/1‑140 (MQ=255)                                                                                             acGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAatat                                            >  1:252013/1‑140 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                           >  1:439124/1‑140 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                           >  2:820671/1‑140 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                           >  2:486319/1‑140 (MQ=255)                                                                                              cGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATAtt                                           >  1:577294/1‑140 (MQ=255)                                                                                               gCCACCGTTATCCGGCGGAGTTCTC‑‑‑TGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:1012045/140‑1 (MQ=255)                                                                                                ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                         <  2:27362/140‑1 (MQ=255)                                                                                                ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                         <  2:635677/140‑1 (MQ=255)                                                                                                ccACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGa                                         <  2:1016427/140‑1 (MQ=255)                                                                                                  aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  1:312417/140‑1 (MQ=255)                                                                                                  aCCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACg                                       <  2:252013/140‑1 (MQ=255)                                                                                                     gTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTa                                    <  1:629091/140‑1 (MQ=255)                                                                                                              cggAGTTCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCa                           >  2:196324/1‑140 (MQ=255)                                                                                                                   ttCTCTGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGca                      <  2:439124/140‑1 (MQ=255)                                                                                                                      tctGGTGGTCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGcaaca                   <  2:750056/140‑1 (MQ=255)                                                                                                                             gtCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCAt            >  1:661861/1‑140 (MQ=255)                                                                                                                              tCAGCGACAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATc           <  1:486319/140‑1 (MQ=255)                                                                                                                                     cAACGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGc    <  1:322066/140‑1 (MQ=255)                                                                                                                                       aCGCATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCt  <  2:477568/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GGCTTACGGGTTCACCAGCCGACGCTTTCTGCCGCACAACGCGAACAACAAGTGATAGCCGTGATGCATGAAGTGGGATTAGATCCTGAAACACGCCACCGTTATCCGGCGGAGTTCTCTGGTGGTCAGCGACAACGTATTGCGATTGCCAGGGCATTAATTCTTAAGCCCTCGCTGATCATACTTGATGAACCGACATCATCACTCGACAAAACGGTACAGGCGCAAATATTGACGCTATTGAAATCATTGCAACAAAAGCATCAACTGGCCT  >  NC_000913/2277461‑2277734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑