Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,338,723	T→C	Y233C (TAC→TGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,338,723	0	T	C	100.0%	167.5 / NA	47	Y233C (TAC→TGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/23);  total (24/23)

GCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCGTTGATGAC  >  NC_000913/2338588‑2338853                                                                                                                                        |                                                                                                                                   gCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGGATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGc                                                                                                                                <  2:834264/140‑1 (MQ=255) gCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGc                                                                                                                                <  1:146559/140‑1 (MQ=255) gCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGc                                                                                                                                <  1:1031830/140‑1 (MQ=255)  cAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGct                                                                                                                               >  1:684383/1‑140 (MQ=255)         ctcGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCaa                                                                                                                        >  1:918237/1‑140 (MQ=255)             aTCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATAcc                                                                                                                    >  2:746877/1‑140 (MQ=255)                   cgcgcTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGcgacg                                                                                                              <  1:196393/140‑1 (MQ=255)                    gcgcTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGcgacga                                                                                                             <  2:918237/140‑1 (MQ=255)                    gcgcTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGcgacga                                                                                                             <  1:369642/140‑1 (MQ=255)                       cTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCg                                                                                                          >  1:671846/1‑140 (MQ=255)                        tttGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGt                                                                                                         >  2:880526/1‑140 (MQ=255)                                ccTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGAtt                                                                                                 >  2:293290/1‑140 (MQ=255)                                    aTACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTgccg                                                                                             >  2:57963/1‑140 (MQ=255)                                     tACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTgccgc                                                                                            >  2:65785/1‑140 (MQ=255)                                      aCGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTgccgcc                                                                                           >  1:35042/1‑140 (MQ=255)                                           aTTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCgg                                                                                      >  2:408789/1‑140 (MQ=255)                                              tCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGaa                                                                                   >  1:47368/1‑140 (MQ=255)                                               cGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAg                                                                                  >  1:617427/1‑140 (MQ=255)                                                 tGGACGATAATGGTTTCACGCCCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTc                                                                                <  1:484330/140‑1 (MQ=255)                                                 tGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTc                                                                                <  1:293290/140‑1 (MQ=255)                                                   gACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCg                                                                              <  2:1014802/140‑1 (MQ=255)                                                         aaTGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCg                                                                        >  2:61949/1‑140 (MQ=255)                                                            ggTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGa                                                                     <  1:408789/140‑1 (MQ=255)                                                                tCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGAtgtg                                                                 >  1:432319/1‑140 (MQ=255)                                                                  aCGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGtt                                                               >  1:776929/1‑140 (MQ=255)                                                                  aCGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGtt                                                               >  1:777564/1‑140 (MQ=255)                                                                         gTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAg                                                        <  1:746877/140‑1 (MQ=255)                                                                         gTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAg                                                        <  2:70351/140‑1 (MQ=255)                                                                              ggCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCtt                                                   <  2:35042/140‑1 (MQ=255)                                                                                    aaCTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAg                                                        <  2:524000/129‑1 (MQ=255)                                                                                    aaCTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAg                                                        >  1:524000/1‑129 (MQ=255)                                                                                    aaCTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCaa                                                <  2:953870/137‑1 (MQ=255)                                                                                    aaCTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCaa                                                >  1:953870/1‑137 (MQ=255)                                                                                      cTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTg                                           <  1:65785/140‑1 (MQ=255)                                                                                         ccACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATg                                        <  2:776929/140‑1 (MQ=255)                                                                                              tCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTtc                                   <  1:284972/140‑1 (MQ=255)                                                                                                   gcgAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTtcatcat                              <  2:684383/140‑1 (MQ=255)                                                                                                   gcgAGCGCGGATATACACCTTGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTtcatcat                              <  2:671846/140‑1 (MQ=255)                                                                                                                       tGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCg          <  1:880526/140‑1 (MQ=255)                                                                                                                       tGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCg          >  2:394666/1‑140 (MQ=255)                                                                                                                       tGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCg          >  1:830236/1‑140 (MQ=255)                                                                                                                       tGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCg          >  1:437160/1‑140 (MQ=255)                                                                                                                       tGCCGCGACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCg          >  1:123980/1‑140 (MQ=255)                                                                                                                         ccGCGACCGGTACGGCAAGCTTCTTCAATACTGCGTCGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGCCCCCGGGATGGGTTCCATCAGCCCTTCATTGCGGATGTCTTCATCATCAATATACGCCAGACAACCGtt        >  2:857784/1‑140 (MQ=255)                                                                                                                           gcgACCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCGTtga      >  1:135234/1‑140 (MQ=255)                                                                                                                              aCCGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCGTtgatga   >  2:513403/1‑140 (MQ=255)                                                                                                                               ccGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCGTTGATGAc  <  2:432319/140‑1 (MQ=255)                                                                                                                               ccGGTACGGCAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCGTTGATGAc  <  1:61949/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GCAATCTTCTCGATCAGGCGCGCTTTGTTTACCTGATACGGAATTTCGTGGACGATAATGGTTTCACGACCGGTTTTGGCGTCAACTTCCACTTCTGCGCGAGCGCGGATATACACCTTGCCGCGACCGGTACGGTAAGCTTCTTCAATACCGCGACGACCGTTAATGATTGCCGCCGTCGGGAAGTCCGGCCCCGGGATGTGTTCCATCAGCCCTTCAATGCTGATGTCTTCATCATCAATATACGCCAGACAACCGTTGATGAC  >  NC_000913/2338588‑2338853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑