Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,471,489	T→C	N10S (AAC→AGC)	yfdK ←	CPS‑53 (KpLE1) prophage; putative tail fiber assembly protein YfdK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,471,489	0	T	C	100.0%	174.9 / NA	48	N10S (AAC→AGC)	yfdK	CPS‑53 (KpLE1) prophage; putative tail fiber assembly protein YfdK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

GATACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGTTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGT  >  NC_000913/2471359‑2471620                                                                                                                                   |                                                                                                                                    gATACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcg                                                                                                                            <  2:257334/140‑1 (MQ=255) gATACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcg                                                                                                                            <  2:619200/140‑1 (MQ=255) gATACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcg                                                                                                                            <  1:687155/140‑1 (MQ=255) gATACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcg                                                                                                                            <  1:546257/140‑1 (MQ=255)  aTACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcgc                                                                                                                           <  1:559077/140‑1 (MQ=255)       gATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAat                                                                                                                      <  2:246370/140‑1 (MQ=255)            ttCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAg                                                                                                                               >  1:708109/1‑126 (MQ=255)            ttCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAg                                                                                                                               <  2:708109/126‑1 (MQ=255)             tCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGt                                                                                                                >  2:255958/1‑140 (MQ=255)                  tCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcg                                                                                                                            >  1:678736/1‑123 (MQ=255)                  tCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTcg                                                                                                                            <  2:678736/123‑1 (MQ=255)                  tCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTc                                                                                                           >  1:493412/1‑140 (MQ=255)                    ggcggTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCAt                                                                                                         >  1:420036/1‑140 (MQ=255)                       ggTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCAtttt                                                                                                      <  2:493412/140‑1 (MQ=255)                          aaTCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCa                                                                                                   <  2:487741/140‑1 (MQ=255)                                gAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCAt                                                                                             <  1:206236/140‑1 (MQ=255)                                        aaTATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTaa                                                                                     <  1:325359/140‑1 (MQ=255)                                                    cTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTaa                                                                                     <  1:478793/128‑1 (MQ=255)                                                    cTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTaa                                                                                     >  2:478793/1‑128 (MQ=255)                                                    cTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAaca                                                                         >  1:433239/1‑140 (MQ=255)                                                       atcaACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACAccc                                                                      >  2:906412/1‑140 (MQ=255)                                                        tcaACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCt                                                                     >  1:214348/1‑140 (MQ=255)                                                         caACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTa                                                                    >  1:963219/1‑140 (MQ=255)                                                             ttCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGc                                                                >  1:53743/1‑140 (MQ=255)                                                             ttCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGc                                                                >  1:272442/1‑140 (MQ=255)                                                              tCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAAt                                                                  >  2:103738/1‑137 (MQ=255)                                                              tCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAAt                                                                  <  1:103738/137‑1 (MQ=255)                                                                      atcatcTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGa                                                       >  1:782954/1‑140 (MQ=255)                                                                      atcatcTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGa                                                       <  2:782954/140‑1 (MQ=255)                                                                      atcatcTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGa                                                       >  2:258313/1‑140 (MQ=255)                                                                       tcatcTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGaa                                                      <  2:287848/140‑1 (MQ=255)                                                                           cTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCAc                                                  <  1:73302/140‑1 (MQ=255)                                                                               ccATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGAt                                              >  2:970148/1‑140 (MQ=255)                                                                                cATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTGAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATa                                             >  1:231100/1‑140 (MQ=255)                                                                                cATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATa                                             <  2:69888/140‑1 (MQ=255)                                                                                cATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATa                                             <  2:963219/140‑1 (MQ=255)                                                                                  tGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATAcc                                           >  1:668233/1‑140 (MQ=255)                                                                                   gAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCg                                          <  2:433239/140‑1 (MQ=255)                                                                                    aGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCgg                                         >  1:60060/1‑140 (MQ=255)                                                                                                   aaTCCTCTTTCATCTCCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGGACCGATACCGGGTACGCCTGCTCTgg                          >  2:923493/1‑140 (MQ=255)                                                                                                                 tcCAAGGGATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCAcc            <  1:906412/140‑1 (MQ=255)                                                                                                                      gggATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTc       <  2:272442/140‑1 (MQ=255)                                                                                                                      gggATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTc       <  2:53743/140‑1 (MQ=255)                                                                                                                      gggATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTc       <  1:633735/140‑1 (MQ=255)                                                                                                                      gggATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAAAGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTc       <  1:363342/140‑1 (MQ=255)                                                                                                                       ggATAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTcc      <  1:923493/140‑1 (MQ=255)                                                                                                                          tAGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTgg   >  2:14151/1‑140 (MQ=255)                                                                                                                           aGAAAGAGCTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGt  >  1:91838/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                    GATACGGATTTTTCCTTTCGGCGGTAATCCGGAAAACTCAATATACACTTGCTCATCAACTTCAACAGCATCATCTGGCCATGAGCCAGCTTGCGTGTAATCCTCTTTCATCTCCAAGGGATAGAAAGAGTTTGTAGTCGCGGAATATATGTAATTCATTTTTCACTCCATAAAGTTAAAAGAAATTAACACCCTAATGCGAAAAATGAAGCACCGATACCGGGTACGCCTGCTCTGGAAATAAATTTCACCGGGTCCTGGT  >  NC_000913/2471359‑2471620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑