Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,609,038	C→T	I184I (ATC→ATT)	hyfG →	hydrogenase 4 catalytic subunit HyfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,609,038	0	C	T	100.0%	187.2 / NA	54	I184I (ATC→ATT)	hyfG	hydrogenase 4 catalytic subunit HyfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/26);  total (28/26)

TGCCCGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGA  >  NC_000913/2608905‑2609174                                                                                                                                      |                                                                                                                                         tGCCCGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTc                                                                                                                                    <  2:20673/140‑1 (MQ=255)  gCCCGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCg                                                                                                                                   >  2:722947/1‑140 (MQ=255)     cGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGcc                                                                                                                                <  2:686581/140‑1 (MQ=255)     cGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGcc                                                                                                                                <  1:722947/140‑1 (MQ=255)        tGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCg                                                                                                                                   <  2:642152/134‑1 (MQ=255)        tGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCg                                                                                                                                   >  1:642152/1‑134 (MQ=255)        tGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGc                                                                                                                             >  1:179420/1‑140 (MQ=255)        tGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGc                                                                                                                             >  2:868948/1‑140 (MQ=255)               ccGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCatat                                                                                                                      <  2:179420/140‑1 (MQ=255)                        atGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGa                                                                                                             >  1:954261/1‑140 (MQ=255)                        atGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGa                                                                                                             >  1:752091/1‑140 (MQ=255)                             tCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAAc                                                                                                        <  1:400289/140‑1 (MQ=255)                              ccGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAAcc                                                                                                       >  1:381914/1‑140 (MQ=255)                                gCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCgg                                                                                                     >  1:249658/1‑140 (MQ=255)                                 cTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCggg                                                                                                    >  2:396824/1‑140 (MQ=255)                                 cTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCggg                                                                                                    <  1:382003/140‑1 (MQ=255)                                          gATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCg                                                                                           <  1:515147/140‑1 (MQ=255)                                            tGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCt                                                                                         <  2:752091/140‑1 (MQ=255)                                            tGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCt                                                                                         <  2:765883/140‑1 (MQ=255)                                            tGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCt                                                                                         >  1:460459/1‑140 (MQ=255)                                              cGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCttg                                                                                       <  1:868948/140‑1 (MQ=255)                                                   gATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCttgtttgt                                                                                  >  2:493343/1‑140 (MQ=255)                                                      tATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGa                                                                               <  2:381914/140‑1 (MQ=255)                                                        tCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATg                                                                             >  2:77366/1‑140 (MQ=255)                                                               cgcCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCa                                                                      >  2:872825/1‑140 (MQ=255)                                                                  ccTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAAt                                                                   <  2:249658/140‑1 (MQ=255)                                                                     gAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGt                                                                <  1:391515/140‑1 (MQ=255)                                                                     gAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGt                                                                <  2:870730/140‑1 (MQ=255)                                                                         cgacgaCTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGAt                                                            <  2:1009504/140‑1 (MQ=255)                                                                          gacgacTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATg                                                           <  2:954261/140‑1 (MQ=255)                                                                           acgacTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGc                                                          >  1:7366/1‑140 (MQ=255)                                                                                tGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCCCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGAtt                                                     >  2:755473/1‑140 (MQ=255)                                                                                  aTTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTAc                                                   <  2:7366/140‑1 (MQ=255)                                                                                         aaCGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGt                                            >  1:338517/1‑140 (MQ=255)                                                                                              aTCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTAt                                       >  1:940595/1‑140 (MQ=255)                                                                                                ccGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCGCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGt                                     >  1:559412/1‑140 (MQ=255)                                                                                                   tttATCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCa                                  <  1:493343/140‑1 (MQ=255)                                                                                                      aTCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATcg                               >  2:483798/1‑140 (MQ=255)                                                                                                      aTCAATGAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATcg                               >  2:157788/1‑140 (MQ=255)                                                                                                      aTCAATAAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGAGGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATcg                               <  2:745614/140‑1 (MQ=255)                                                                                                            gAGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCAt                         >  1:306008/1‑140 (MQ=255)                                                                                                             aGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGGTTACCGCCTGTTTTATGTCCATCGCGGCATg                        >  2:398447/1‑140 (MQ=255)                                                                                                             aGGGCAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATg                        >  2:706369/1‑140 (MQ=255)                                                                                                                 cAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGc                           <  2:837973/133‑1 (MQ=255)                                                                                                                 cAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGc                           >  1:837973/1‑133 (MQ=255)                                                                                                                 cAACAGCGATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGgaga                    >  1:109470/1‑140 (MQ=255)                                                                                                                        gATGCGCGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGc             <  2:306008/140‑1 (MQ=255)                                                                                                                           gcgcGGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGa          >  2:372942/1‑140 (MQ=255)                                                                                                                             gcgGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGaaa        <  1:157788/140‑1 (MQ=255)                                                                                                                              cgGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAAc       >  1:698704/1‑140 (MQ=255)                                                                                                                              cgGGTGATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAAc       >  2:462215/1‑140 (MQ=255)                                                                                                                                   gATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGa  <  1:48650/140‑1 (MQ=255)                                                                                                                                   gATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGa  <  2:109470/140‑1 (MQ=255)                                                                                                                                   gATTCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGa  <  2:295064/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TGCCCGATGACTGGCCGGAAGATATGCATCCGCTGCGCAAAGATGCGATGGATTATCGACTGCGCCCTGAACCGACGACTGATTCCGAAACGTATCCGTTTATCAATGAGGGCAACAGCGATGCGCGGGTGATCCCTGTCGGCCCGCTGCATATCACCTCCGATGAACCGGGTCACTTCCGCTTGTTTGTGGATGGCGAGCAAATTGTCGATGCTGATTACCGCCTGTTTTATGTCCATCGCGGCATGGAGAAACTGGCAGAAACGCGGA  >  NC_000913/2608905‑2609174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑