Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 187,029 A→G L541P (CTG→CCG)  glnD ← PII uridylyltransferase/uridylyl removing enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913187,0290AG97.1% 119.6 / ‑4.8 35L541P (CTG→CCG) glnDPII uridylyltransferase/uridylyl removing enzyme
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (15/19);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTCCGTTTGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGA  >  NC_000913/186897‑187161
                                                                                                                                    |                                                                                                                                    
ttttCCGTTTGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTg                                                                                                                               <  2:541375/140‑1 (MQ=255)
        ttGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTTgg                                                                                                                       >  1:433741/1‑140 (MQ=255)
            aCTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTTGGCaaa                                                                                                                   <  2:433741/140‑1 (MQ=255)
                  tcGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCAc                                                                                                             <  2:343768/140‑1 (MQ=255)
                   cGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACt                                                                                                            <  1:220862/140‑1 (MQ=255)
                   cGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACt                                                                                                            <  1:288047/140‑1 (MQ=255)
                     gCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTAc                                                                                                          <  1:612349/140‑1 (MQ=255)
                                 atgaCTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCAcc                                                                                              <  1:706785/140‑1 (MQ=255)
                                     cTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCgaga                                                                                          >  2:734277/1‑140 (MQ=255)
                                                 gAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTcg                                                                              <  2:377079/140‑1 (MQ=255)
                                                       cgcgGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTcgccgccg                                                                        >  2:590970/1‑140 (MQ=255)
                                                       cgcgGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTcgccgccg                                                                        >  2:815057/1‑140 (MQ=255)
                                                       cgcgGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTcgccgccg                                                                        >  1:270746/1‑140 (MQ=255)
                                                          ggcgTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGt                                                                     <  1:815057/140‑1 (MQ=255)
                                                                    ggTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGa                                                           <  1:590970/140‑1 (MQ=255)
                                                                    ggTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGa                                                           <  1:281774/140‑1 (MQ=255)
                                                                            aCATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGa                                                   >  1:824853/1‑140 (MQ=255)
                                                                                   cAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAgcg                                            >  2:156046/1‑140 (MQ=255)
                                                                                      gTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGa                                                           <  2:563694/122‑1 (MQ=255)
                                                                                      gTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGa                                                           >  1:563694/1‑122 (MQ=255)
                                                                                      gTGCTGGAGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCTGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGGGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCgcgg                                         >  2:679711/1‑140 (MQ=255)
                                                                                        gCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCgcggcg                                       >  1:1008859/1‑140 (MQ=255)
                                                                                                 ccagccagGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATc                              >  1:779774/1‑140 (MQ=255)
                                                                                                    gccagGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGc                           <  2:736361/140‑1 (MQ=255)
                                                                                                     ccagGCGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCt                          <  2:981807/140‑1 (MQ=255)
                                                                                                          cGACCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGtt                     <  2:779774/140‑1 (MQ=255)
                                                                                                            aCCAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGa                   >  2:89664/1‑140 (MQ=255)
                                                                                                              cAGCTGTGTTTCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACg                 >  2:910601/1‑140 (MQ=255)
                                                                                                                      tttCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGgcgc         >  2:938763/1‑140 (MQ=255)
                                                                                                                      tttCGCGTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGAGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGgcgc         >  2:917529/1‑140 (MQ=255)
                                                                                                                         cgcgTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGc      <  1:734277/140‑1 (MQ=255)
                                                                                                                           cgTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGgc           <  2:469527/133‑1 (MQ=255)
                                                                                                                           cgTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGgc           >  1:469527/1‑133 (MQ=255)
                                                                                                                           cgTGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCa    <  1:156046/140‑1 (MQ=255)
                                                                                                                             tGAGTTCGGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGa  <  2:270746/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                    
TTTTCCGTTTGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCGGCAAAATGCACTACATCCTGAGCACCGAGAATGGAGTGGTCGCCGCCGCGTCCTTTGGCGATATCGTGAAACAGCGCGGCGATGAAAATCAGCTCAGTTGACGGCAGGCGCGGCCAGA  >  NC_000913/186897‑187161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑