Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,659,669	T→C	D73G (GAC→GGC)	iscA ←	iron‑sulfur cluster insertion protein IscA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,659,669	0	T	C	100.0%	151.1 / NA	43	D73G (GAC→GGC)	iscA	iron‑sulfur cluster insertion protein IscA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/23);  total (20/23)

GGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATACCTGAACACCCG  >  NC_000913/2659532‑2659788                                                                                                                                          |                                                                                                                        ggCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGccc                                                                                                                       >  2:768234/1‑140 (MQ=255)       cACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAAt                                                                                                                 >  2:600550/1‑140 (MQ=255)            tGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAg                                                                                                            >  2:370549/1‑140 (MQ=255)                gTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGctc                                                                                                        >  1:1015917/1‑140 (MQ=255)                gTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGctc                                                                                                        <  1:307017/140‑1 (MQ=255)                 ttATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGctct                                                                                                       <  1:768234/140‑1 (MQ=255)                    tCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGc                                                                                                    >  1:326947/1‑140 (MQ=255)                                 cAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGAc                                                                                       >  1:982254/1‑140 (MQ=255)                                             cTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCttt                                                                           <  2:982254/140‑1 (MQ=255)                                                  gccgcAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCtt                                                                      <  2:878644/140‑1 (MQ=255)                                                     gcAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCaa                                                                   <  1:1006812/140‑1 (MQ=255)                                                        aCCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAaca                                                                >  2:838963/1‑140 (MQ=255)                                                          cacaCTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACg                                                              <  2:1015917/140‑1 (MQ=255)                                                            cacTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGAt                                                            <  1:838963/140‑1 (MQ=255)                                                              cTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGt                                                          >  2:539222/1‑140 (MQ=255)                                                                 aTCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCtt                                                       >  2:761826/1‑140 (MQ=255)                                                                 aTCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCtt                                                       >  1:52686/1‑140 (MQ=255)                                                                   cTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTcc                                                     >  1:343102/1‑140 (MQ=255)                                                                   cTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTcc                                                     >  1:327979/1‑140 (MQ=255)                                                                      tGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGc                                                  >  1:63084/1‑140 (MQ=255)                                                                      tGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGc                                                  <  2:734799/140‑1 (MQ=255)                                                                       gACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCg                                                 >  1:494105/1‑140 (MQ=255)                                                                         cGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTc                                               <  1:36220/140‑1 (MQ=255)                                                                           ttCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCgg                                             >  2:54268/1‑140 (MQ=255)                                                                           ttCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCgg                                             >  2:528622/1‑140 (MQ=255)                                                                           ttCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCgg                                             >  2:151530/1‑140 (MQ=255)                                                                                   ggTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTcaa                                     <  1:151530/140‑1 (MQ=255)                                                                                     tGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTcaaca                                   <  2:52686/140‑1 (MQ=255)                                                                                          ttAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTc                              <  2:327979/140‑1 (MQ=255)                                                                                          ttAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTc                              >  2:72580/1‑140 (MQ=255)                                                                                            aaaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCa                            <  1:761826/140‑1 (MQ=255)                                                                                            aaaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCa                            <  2:494105/140‑1 (MQ=255)                                                                                            aaaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCa                            <  1:54268/140‑1 (MQ=255)                                                                                            aaaCCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCa                            <  2:63084/140‑1 (MQ=255)                                                                                                  ttCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACAt                      >  1:69417/1‑140 (MQ=255)                                                                                                      ttCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGc                  <  1:528622/140‑1 (MQ=255)                                                                                                       tCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGcc                 <  1:72580/140‑1 (MQ=255)                                                                                                         aGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCAt               <  2:343102/140‑1 (MQ=255)                                                                                                          ggCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATa              >  2:197729/1‑140 (MQ=255)                                                                                                          ggCCTTCTTTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATa              <  2:851381/140‑1 (MQ=255)                                                                                                               tcttTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATACCTGa         <  2:326947/140‑1 (MQ=255)                                                                                                                cttTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATACCTGa         >  1:683846/1‑139 (MQ=255)                                                                                                                cttTTACGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATACCTGa         <  2:683846/139‑1 (MQ=255)                                                                                                                      cGAAGTCCAGCTGCGTACCGCCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATACCTGAACACCCg  <  2:868517/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                        GGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCTTCGTTCAGGCCTTCTTTTACGAAGTCCAGCTGCGTACCGTCCAGAAATTGCAGGCTCTTGCCATCGACCACGACTTTCACGCCTTTGTCTTCAAACACGATGTCTTCCGGCGTCGGTTCGTCAACAAATTCCAGTACATAAGCCATACCTGAACACCCG  >  NC_000913/2659532‑2659788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑