Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,706,133	G→A	S331F (TCC→TTC)	lepA ←	elongation factor 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,706,133	0	G	A	100.0%	190.3 / NA	53	S331F (TCC→TTC)	lepA	elongation factor 4
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/26);  total (27/26)

GGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGC  >  NC_000913/2705997‑2706265                                                                                                                                         |                                                                                                                                     ggTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGc                                                                                                                                   >  1:369395/1‑140 (MQ=255) ggTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGc                                                                                                                                   >  2:894685/1‑140 (MQ=255)  gTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCt                                                                                                                                  >  2:350795/1‑140 (MQ=255)       aaCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCg                                                                                                                             <  2:369395/140‑1 (MQ=255)       aaCTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCg                                                                                                                             <  1:350795/140‑1 (MQ=255)            cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCa                                                                                                                        <  1:286124/140‑1 (MQ=255)            cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCa                                                                                                                        >  2:84362/1‑140 (MQ=255)            cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCa                                                                                                                        <  1:84362/140‑1 (MQ=255)            cATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCa                                                                                                                        >  2:235017/1‑140 (MQ=255)                   tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCg                                                                                                                             <  1:332854/128‑1 (MQ=255)                   tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCg                                                                                                                             >  2:889965/1‑128 (MQ=255)                   tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCg                                                                                                                             >  2:332854/1‑128 (MQ=255)                   tACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCg                                                                                                                             <  1:889965/128‑1 (MQ=255)                      ggTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTg                                                                                                              >  1:270391/1‑140 (MQ=255)                      ggTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTg                                                                                                              >  2:993413/1‑140 (MQ=255)                      ggTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTg                                                                                                              >  1:689775/1‑140 (MQ=255)                         cGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGaa                                                                                                                   <  1:422942/132‑1 (MQ=255)                         cGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGaa                                                                                                                   >  2:422942/1‑132 (MQ=255)                           gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGc                                                                                                                           <  2:972576/122‑1 (MQ=255)                           gcgcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGc                                                                                                                           >  1:972576/1‑122 (MQ=255)                             gcAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATc                                                                                                       >  2:1009076/1‑140 (MQ=255)                               aGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGt                                                                                                     <  2:689775/140‑1 (MQ=255)                                     gATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                                               <  1:1009076/140‑1 (MQ=255)                                          gatccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGt                                                                                          <  2:336306/140‑1 (MQ=255)                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGt                                                                                                     <  2:493417/128‑1 (MQ=255)                                           atccagatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGt                                                                                                     >  1:493417/1‑128 (MQ=255)                                                gatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                                               >  1:532044/1‑129 (MQ=255)                                                gatcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                                               <  2:532044/129‑1 (MQ=255)                                                 atcGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCa                                                                                   <  1:10248/140‑1 (MQ=255)                                                     tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAgcgc                                                                               >  1:873930/1‑140 (MQ=255)                                                     tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAg                                                                                  <  2:215450/137‑1 (MQ=255)                                                     tATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAg                                                                                  >  1:215450/1‑137 (MQ=255)                                                      aTTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                                               >  2:343064/1‑123 (MQ=255)                                                      aTTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGc                                                                                               <  1:343064/123‑1 (MQ=255)                                                             ttCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGa                                                                       <  1:459884/140‑1 (MQ=255)                                                              tCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa                                                                      <  1:949617/140‑1 (MQ=255)                                                              tCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGaa                                                                      <  2:873930/140‑1 (MQ=255)                                                               ccAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAg                                                                     >  1:47087/1‑140 (MQ=255)                                                                        ccTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATTGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAg                                                            >  2:341593/1‑140 (MQ=255)                                                                                          gcagcaGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCggg                                          <  1:893919/140‑1 (MQ=255)                                                                                           cagcagGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGa                                         >  1:978290/1‑140 (MQ=255)                                                                                           cagcagGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGa                                         >  2:89279/1‑140 (MQ=255)                                                                                            agcagGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGaa                                        <  1:993413/140‑1 (MQ=255)                                                                                            agcagGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGaa                                        <  2:47087/140‑1 (MQ=255)                                                                                             gcagGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAAc                                       >  2:855058/1‑140 (MQ=255)                                                                                                 gCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGAc                                   >  1:166798/1‑140 (MQ=255)                                                                                                      ggAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACAGGCg                              <  2:9406/140‑1 (MQ=255)                                                                                                           ccGCAGCGGAAACCAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATAc                         >  1:16636/1‑140 (MQ=255)                                                                                                                       ccAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGAc             <  1:89279/140‑1 (MQ=255)                                                                                                                        cAAAGCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACt            <  2:16636/140‑1 (MQ=255)                                                                                                                            gCCCAGCGCGCTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCt        <  2:270391/140‑1 (MQ=255)                                                                                                                                 gcgcgcTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAg   >  2:754163/1‑140 (MQ=255)                                                                                                                                  cgcgcTGAAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGc  >  1:198134/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GGTTTCAACTTCATACACTACGGTCGGCGCAGTGGTGATCAGATCCAGATCGTATTCACGTTCCAGACGTTCCTGGATGATCTCCATGTGCAGCAGGCCGAGGAAGCCGCAGCGGAAACCAAAGCCCAGCGCGCTGGAGCTTTCCGGCTCATAGAACAGTGAGGCATCGTTCAGGCTGAGTTTACCCAGCGCGTCACGGAAGGCTTCATAGTCGTCGGAACTTACCGGGAACAGACCGGCGTATACCTGCGGTTTGACTTTCTTAAAGC  >  NC_000913/2705997‑2706265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑