Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,719,833	A→G	E204G (GAG→GGG)	yfiP →	conserved protein YfiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,719,833	0	A	G	100.0%	185.2 / NA	51	E204G (GAG→GGG)	yfiP	conserved protein YfiP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

CGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGAGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGCGAGGACT  >  NC_000913/2719705‑2719969                                                                                                                                 |                                                                                                                                         cGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTAccc                                                                                                                               >  2:444076/1‑140 (MQ=255)       ttCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTAcc                                                                                                                                <  1:817628/133‑1 (MQ=255)       ttCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTAcc                                                                                                                                >  2:817628/1‑133 (MQ=255)         cccGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCACTTTaaa                                                                                                                       <  2:651541/140‑1 (MQ=255)            ggCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAaca                                                                                                                    <  2:681644/140‑1 (MQ=255)                 tCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTTCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTa                                                                                                               >  2:184492/1‑140 (MQ=255)                     ccTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTg                                                                                                           <  1:184789/140‑1 (MQ=255)                       tATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGc                                                                                                         <  1:912708/140‑1 (MQ=255)                          cGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAgg                                                                                                      >  1:378090/1‑140 (MQ=255)                               gCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGaaaaa                                                                                                 >  2:195424/1‑140 (MQ=255)                                  tGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGc                                                                                              <  2:788048/140‑1 (MQ=255)                                  tGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGc                                                                                              <  2:4919/140‑1 (MQ=255)                                       cccAGGCTGAAGGCCAATATTGTACTTCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACAt                                                                                         <  1:184492/140‑1 (MQ=255)                                             cTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGt                                                                                   <  2:76448/140‑1 (MQ=255)                                                          ttGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCAcagcag                                                                      <  1:572760/140‑1 (MQ=255)                                                          ttGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCAcagcag                                                                      <  1:195424/140‑1 (MQ=255)                                                            gTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGaa                                                                    <  2:592426/140‑1 (MQ=255)                                                            gTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGaa                                                                    >  1:496051/1‑140 (MQ=255)                                                             tACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCGGGGTTAGGCGGGCTTTTTACCCGCTTTAAAACACGCTATTTGGCGGGAAAAACCCAACATCTGGGAAGCATCACAGCAGaaa                                                                   >  2:61752/1‑139 (MQ=255)                                                             tACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAAc                                                                   >  1:406316/1‑140 (MQ=255)                                                                  ccGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCAcagc                                                                        >  1:58180/1‑130 (MQ=255)                                                                  ccGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCAcagc                                                                        <  2:58180/130‑1 (MQ=255)                                                                  ccGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTa                                                              >  2:898369/1‑140 (MQ=255)                                                                  ccGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTa                                                              >  2:488541/1‑140 (MQ=255)                                                                    gAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCAt                                                                              >  2:730706/1‑122 (MQ=255)                                                                    gAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCAt                                                                              <  1:730706/122‑1 (MQ=255)                                                                     aGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATTTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGaa                                                           >  2:170177/1‑140 (MQ=255)                                                                               cGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGtt                                                     <  2:756302/136‑1 (MQ=255)                                                                               cGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGtt                                                     >  1:756302/1‑136 (MQ=255)                                                                                      ttAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTc                                          >  1:954862/1‑140 (MQ=255)                                                                                              ggCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCg                                         <  2:244353/133‑1 (MQ=255)                                                                                              ggCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCg                                         >  1:244353/1‑133 (MQ=255)                                                                                               gCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTc                                 >  2:123732/1‑140 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGt                                       >  1:985049/1‑133 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGt                                       <  2:985049/133‑1 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCt                                >  2:298928/1‑140 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCt                                >  2:207186/1‑140 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCt                                >  2:677351/1‑140 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCt                                >  1:684583/1‑140 (MQ=255)                                                                                                ccGGCGATACCGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTCAGAAAGCGTTTAAAATCATTCGGTCACTTCt                                >  2:975562/1‑140 (MQ=255)                                                                                                         ccGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGAc                       <  2:406316/140‑1 (MQ=255)                                                                                                         ccGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGAc                       <  1:444076/140‑1 (MQ=255)                                                                                                         ccGGGGCGGCGGCAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGAc                       <  1:170177/140‑1 (MQ=255)                                                                                                             ggcggcggcAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGt                   >  2:941420/1‑140 (MQ=255)                                                                                                              gcggcggcAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTa                  <  2:93576/140‑1 (MQ=255)                                                                                                                 gcggcAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGa               >  1:252415/1‑140 (MQ=255)                                                                                                                  cggcAGGGTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAg              <  2:67776/140‑1 (MQ=255)                                                                                                                       gggTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGGAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGc         <  2:378090/140‑1 (MQ=255)                                                                                                                        ggTTAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGCg        <  1:941420/140‑1 (MQ=255)                                                                                                                          ttAGGCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGCGAg      <  2:954862/140‑1 (MQ=255)                                                                                                                              gCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGCGAGGACt  <  1:975562/140‑1 (MQ=255)                                                                                                                              gCGGGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGCGAGGACt  <  2:684583/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         CGATCTTTCCCGGCTTTCTGCCTATCGCCTGCGTGAAGCCCAGGCTGAAGGCCAATATTGTACTGCCGAGGTAGCCATCGCACTGTTAGATATGGCCGGCGATACCGGGGCGGCGGCAGGGTTAGGCGAGCATTTTACCCGCTTTAAAACACGCTATCTGGCAGGAAAAACGCAACATCTGGGTAGCATCACAGCAGAACAGTTAGAAAGCGTTTAAAATCATTCGGTCACTTCTGCGGGAGACCGGTATGAGTCAGCGAGGACT  >  NC_000913/2719705‑2719969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑