Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,867	T→C	Y375H (TAC→CAC)	pheA →	fused chorismate mutase/prephenate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,867	0	T	C	100.0%	209.6 / NA	58	Y375H (TAC→CAC)	pheA	fused chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/31);  total (27/31)

CACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGA  >  NC_000913/2738733‑2738999                                                                                                                                       |                                                                                                                                     cACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCa                                                                                                                                 <  2:578136/140‑1 (MQ=255)   cGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAg                                                                                                                               >  1:988182/1‑140 (MQ=255)   cGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAg                                                                                                                               >  1:361289/1‑140 (MQ=255)   cGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAg                                                                                                                               >  2:429536/1‑140 (MQ=255)       cGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTgag                                                                                                                           <  1:892350/140‑1 (MQ=255)       cGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTgag                                                                                                                           >  1:354874/1‑140 (MQ=255)           tCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACg                                                                                                                       <  1:197430/140‑1 (MQ=255)            cACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGt                                                                                                                      <  1:716093/140‑1 (MQ=255)               ggTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGt                                                                                                                   <  2:961176/140‑1 (MQ=255)                    tCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTg                                                                                                              >  2:154756/1‑140 (MQ=255)                    tCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTg                                                                                                              <  2:354874/140‑1 (MQ=255)                    tCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTg                                                                                                              <  2:431951/140‑1 (MQ=255)                        tGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGt                                                                                                             <  2:356636/137‑1 (MQ=255)                        tGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGt                                                                                                             >  1:356636/1‑137 (MQ=255)                         gggAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTg                                                                                                              >  1:670702/1‑135 (MQ=255)                         gggAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTg                                                                                                              <  2:670702/135‑1 (MQ=255)                           gAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATcc                                                                                                       >  1:872011/1‑140 (MQ=255)                           gAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATc                                                                                                        >  2:1019204/1‑139 (MQ=255)                           gAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATc                                                                                                        <  1:1019204/139‑1 (MQ=255)                             agagATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCaa                                                                                                     >  2:177025/1‑140 (MQ=255)                             agagATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCaa                                                                                                     <  1:654529/140‑1 (MQ=255)                             agagATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCaa                                                                                                     >  1:181790/1‑140 (MQ=255)                              gagaTGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAc                                                                                                    <  2:643234/140‑1 (MQ=255)                               agaTGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAcc                                                                                                   <  2:181790/140‑1 (MQ=255)                               agaTGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAcc                                                                                                   <  2:55193/140‑1 (MQ=255)                                   gTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCtgat                                                                                               <  1:700099/140‑1 (MQ=255)                                       tATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGaaa                                                                                           <  2:988182/140‑1 (MQ=255)                                         tCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAg                                                                                         >  1:1031287/1‑140 (MQ=255)                                         tCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAg                                                                                         >  1:530874/1‑140 (MQ=255)                                              atatTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAcc                                                                                                   <  1:280311/125‑1 (MQ=255)                                              atatTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAcc                                                                                                   >  2:280311/1‑125 (MQ=255)                                              atatTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGcc                                                                                    >  2:867154/1‑140 (MQ=255)                                                atTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCgg                                                                                  <  1:867154/140‑1 (MQ=255)                                                           tCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAat                                                                       >  1:786248/1‑140 (MQ=255)                                                           tCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAat                                                                       >  2:683627/1‑140 (MQ=255)                                                                    aGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCa                                                              <  1:1000520/140‑1 (MQ=255)                                                                    aGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCa                                                              >  1:225019/1‑140 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             >  1:345418/1‑140 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  2:786248/140‑1 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  2:384916/140‑1 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             >  1:309970/1‑140 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  1:631187/140‑1 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  1:154756/140‑1 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  1:177025/140‑1 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  1:186406/140‑1 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             >  2:591896/1‑140 (MQ=255)                                                                     gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTAGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCAc                                                             <  1:429536/140‑1 (MQ=255)                                                                      cGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGaa                                                                        <  1:411429/128‑1 (MQ=255)                                                                      cGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGaa                                                                        >  2:411429/1‑128 (MQ=255)                                                                              cAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCa                                                              <  2:442305/130‑1 (MQ=255)                                                                              cAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCa                                                              >  1:442305/1‑130 (MQ=255)                                                                                      aTTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGa                                            >  1:998543/1‑140 (MQ=255)                                                                                                  aGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGcc                                <  2:225019/140‑1 (MQ=255)                                                                                                  aGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGcc                                <  2:361289/140‑1 (MQ=255)                                                                                                   ggggAAATCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCt                               <  2:670432/140‑1 (MQ=255)                                                                                                        aaTCACCCGTTCAATGAAGGTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGc                          >  2:139468/1‑140 (MQ=255)                                                                                                                           gTATTGGGCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCAc       >  1:780506/1‑140 (MQ=255)                                                                                                                                gggCTGTCACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTCCACTCTGa  >  1:961772/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CACGCCCGATTCACGGTAATCCATGGGAAGAGATGTTCTATCTGGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGA  >  NC_000913/2738733‑2738999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑