Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,744,734	G→A	A202A (GCC→GCT)	trmD ←	tRNA m(1)G37 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,744,734	0	G	A	100.0%	163.1 / NA	46	A202A (GCC→GCT)	trmD	tRNA m(1)G37 methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/30);  total (16/30)

GCTGTTGTGCGTGTTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCGGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGT  >  NC_000913/2744598‑2744863                                                                                                                                         |                                                                                                                                  gCTGTTGTGCGTGTTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCa                                                                                                                                >  1:174448/1‑140 (MQ=255)  cTGTTGTGCGTGTTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCAt                                                                                                                               >  2:82754/1‑140 (MQ=255)  cTGTTGTGCGTGTTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCAt                                                                                                                               >  2:106605/1‑140 (MQ=255)          cGTGTTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGccc                                                                                                                       <  1:105047/140‑1 (MQ=255)            tgtTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGa                                                                                                                     <  2:684211/140‑1 (MQ=255)            tgtTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGa                                                                                                                     >  2:495354/1‑140 (MQ=255)               tCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAg                                                                                                                  >  2:104526/1‑140 (MQ=255)               tCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAg                                                                                                                  >  1:776925/1‑140 (MQ=255)                   ttttGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAac                                                                                                              <  2:174448/140‑1 (MQ=255)                      tGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAg                                                                                                                  >  2:933936/1‑133 (MQ=255)                      tGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAg                                                                                                                  <  1:933936/133‑1 (MQ=255)                           tCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCgg                                                                                                      <  1:343057/140‑1 (MQ=255)                               ccAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACt                                                                                                  >  2:54179/1‑140 (MQ=255)                                  agtaCCTTGCTTACTATTCAGTCAGAGCCAGATTTTACAGAAGTTAAGGTCTACTAAGCCAGGTACGGCCCAGCGACTATTCCAAACGCCAGCAACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTcc                                                                                               <  2:169938/137‑1 (MQ=255)                                   cAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAGCACTGGCGGAACTTCCa                                                                                              <  2:776925/140‑1 (MQ=255)                                   cAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCa                                                                                              <  1:495354/140‑1 (MQ=255)                                     aCCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATc                                                                                            <  1:51980/140‑1 (MQ=255)                                     aCCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATc                                                                                            <  1:747847/140‑1 (MQ=255)                                      ccTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATcc                                                                                           <  1:54179/140‑1 (MQ=255)                                      ccTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATcc                                                                                           <  1:351594/140‑1 (MQ=255)                                             tgctCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTaa                                                                                    <  2:886528/140‑1 (MQ=255)                                                 ctTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACAcc                                                                                >  1:894976/1‑140 (MQ=255)                                                 ctTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACAcc                                                                                <  1:96589/140‑1 (MQ=255)                                                  ttCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTATGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCAt                                                                                             <  1:858113/126‑1 (MQ=255)                                                  ttCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTATGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCAt                                                                                             >  2:858113/1‑126 (MQ=255)                                                   tcagtcagAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTc                                                                              <  1:869988/140‑1 (MQ=255)                                                   tcagtcagAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTc                                                                              <  1:281075/140‑1 (MQ=255)                                                   tcagtcagAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTc                                                                              <  2:218305/140‑1 (MQ=255)                                                     agtcagAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAg                                                                            <  1:82754/140‑1 (MQ=255)                                                       tcagAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGc                                                                          <  1:106605/140‑1 (MQ=255)                                                          gagCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCcgc                                                                       >  2:689551/1‑140 (MQ=255)                                                          gagCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCcgc                                                                       >  1:163574/1‑140 (MQ=255)                                                                      ccAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCggg                                                           <  1:760864/140‑1 (MQ=255)                                                                               cAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGc                                                  <  2:163574/140‑1 (MQ=255)                                                                                      tctAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCtt                                           >  2:902645/1‑140 (MQ=255)                                                                                       ctAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTc                                          <  2:1016642/140‑1 (MQ=255)                                                                                       ctAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTc                                          >  1:131059/1‑140 (MQ=255)                                                                                                 tACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGaa                                >  2:676558/1‑140 (MQ=255)                                                                                                         aGCGACTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGt                        >  1:480498/1‑140 (MQ=255)                                                                                                             aCTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGcc                    <  1:902645/140‑1 (MQ=255)                                                                                                             aCTGTTTCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGcc                    <  1:679793/140‑1 (MQ=255)                                                                                                                   tCAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCt              <  1:104526/140‑1 (MQ=255)                                                                                                                    cAAACGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCtt             <  1:676558/140‑1 (MQ=255)                                                                                                                      aaCGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCa           <  2:480498/140‑1 (MQ=255)                                                                                                                      aaCGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCa           <  2:667702/140‑1 (MQ=255)                                                                                                                      aaCGCCAGCGACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCa           <  2:894976/140‑1 (MQ=255)                                                                                                                               gACGTATCTCAGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGt  <  1:548831/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GCTGTTGTGCGTGTTCCGTTTTGAACTCCGCCAGCAACCTTGCTTGCTCTTCAGTCAGAGCCAGGTTTTCCAGAAGTTCAGGTCTTCTAAGCCAGGTACGGCCCAGCGACTGTTTCAAACGCCAGCGACGTATCTCGGCATGGTTGCCCGACAGTAACACTGGCGGAACTTCCATCCCTTCTAACACCTCAGGCCGCGTATAGTGCGGGCAATCCAGCAATCCTTCAGCAAAGGAATCTTCCGTTGCCGAGGCTTCATGTCCCAGT  >  NC_000913/2744598‑2744863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑