Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	175,305	(G)5→4	coding (199/1422 nt)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	175,301	0	G	.	100.0%	121.7 / NA	29	coding (195/1422 nt)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (11/18);  total (11/18)

GACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCC  >  NC_000913/175162‑175435                                                                                                                                            |                                                                                                                                       gACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTTACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATg                                                                                                                                        <  2:105106/140‑1 (MQ=255)  gcaatttaTTCGGCAACTTCTTGGGCGCGATAAAACCCCGTTAGCCCTTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATgg                                                                                                                                       <  2:696939/133‑1 (MQ=255)    aaCTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATgggg                                                                                                                                     <  2:351473/140‑1 (MQ=255)      cTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATggggcg                                                                                                                                   <  2:61252/140‑3 (MQ=255)      cTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATggggcg                                                                                                                                   <  2:301950/140‑3 (MQ=255)      cTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATggggcg                                                                                                                                   <  2:164800/140‑3 (MQ=255)                aaCTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATa                                                                                                                        <  2:789514/140‑1 (MQ=255)                      ttGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATActgctg                                                                                                                  <  2:497797/140‑1 (MQ=255)                               aTAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATc                                                                                                         <  1:1033821/140‑1 (MQ=255)                               aTAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATc                                                                                                         <  2:716473/140‑1 (MQ=255)                                 aaaaCCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCg                                                                                                       <  2:727126/140‑1 (MQ=255)                                  aaaCCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGc                                                                                                      <  1:1339595/140‑1 (MQ=255)                                                   ttGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTc                                                                                        >  1:346859/1‑137 (MQ=255)                                                   ttGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTc                                                                                        <  2:346859/137‑1 (MQ=255)                                                        tATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTtct                                                                                <  2:826556/140‑1 (MQ=255)                                                        tATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTtct                                                                                <  2:479403/140‑1 (MQ=255)                                                            gcggcAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGt                                                                            >  1:974075/1‑140 (MQ=255)                                                                   tcgtcgGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTcggcgg                                                                     <  2:318935/140‑1 (MQ=255)                                                                       cgGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCt                                                                 >  1:698296/1‑140 (MQ=255)                                                                           aCGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCg                                                             <  2:924341/140‑1 (MQ=255)                                                                            cGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGa                                                            <  2:1099911/140‑1 (MQ=255)                                                                                  ttgGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTg                                                      >  1:174297/1‑140 (MQ=255)                                                                                   tgGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTgg                                                     <  2:974075/140‑1 (MQ=255)                                                                                              gggTTGCTTTTGACAAAGGTGTCTCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATGCTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGAGATGTTTGGCTACTTTTTg                                           <  2:454639/139‑1 (MQ=255)                                                                                                    cTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCa                                    >  2:1232883/1‑140 (MQ=255)                                                                                                         gACAAAGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATAc                               >  2:1215221/1‑140 (MQ=255)                                                                                                             aaGGTGTCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACgcgc                           >  1:761494/1‑140 (MQ=255)                                                                                                                   tCGCCTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCGTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAg                     >  2:982546/1‑140 (MQ=255)                                                                                                                       cTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAgg                 <  2:616823/140‑1 (MQ=255)                                                                                                                       cTGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAgg                 <  2:761494/140‑1 (MQ=255)                                                                                                                        tGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACgcgc                           >  2:44854/1‑129 (MQ=255)                                                                                                                        tGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACgcgc                           <  1:44854/129‑1 (MQ=255)                                                                                                                        tGGTTGCAGAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAggt                <  1:1232883/140‑1 (MQ=255)                                                                                                                                gAACCAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCggg        <  1:982546/140‑1 (MQ=255)                                                                                                                                    cAACGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATc    >  1:141454/1‑140 (MQ=255)                                                                                                                                      aCGTAT‑GGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATccc  >  1:990649/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       GACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACCAACGTATGGGGGCGCTGGTACATACTGCTGATAATTATCCGCTTCTGTTAACCGTCGCTTTTCTCTGTTCGGCGGTGCTGGCGATGTTTGGCTACTTTTTGGTGCGCAAATACGCGCCGGAAGCAGGTGGTTCGGGGATCCC  >  NC_000913/175162‑175435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑