Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,769,478	(G)7→6	coding (405/1113 nt)	ydiK →	putative transporter YdiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,769,472	0	G	.	100.0%	130.3 / NA	31	coding (399/1113 nt)	ydiK	putative transporter YdiK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (13/18);  total (13/18)

GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTG  >  NC_000913/1769336‑1769601                                                                                                                                         |                                                                                                                                  gTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATgggg                                                                                                                                >  2:1240/1‑140 (MQ=255)    tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATgggggga                                                                                                                             >  1:1377611/1‑139 (MQ=255)    tcgtcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATgggggga                                                                                                                             >  1:723646/1‑139 (MQ=255)       tcgACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATggggggacgg                                                                                                                          <  2:723646/140‑5 (MQ=255)           cGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGAt                                                                                                                     <  1:1240/140‑1 (MQ=255)            ggCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATc                                                                                                                    >  1:532643/1‑140 (MQ=255)                        cTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGTTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTc                                                                                                        <  2:1278788/140‑1 (MQ=255)                        cTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTc                                                                                                        <  1:998488/140‑1 (MQ=255)                        cTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTc                                                                                                        <  2:414939/140‑1 (MQ=255)                        cTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTc                                                                                                        <  2:532643/140‑1 (MQ=255)                        cTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTc                                                                                                        <  2:555067/140‑1 (MQ=255)                                      ttCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTgg                                                                                          >  2:362843/1‑140 (MQ=255)                                              gTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                                   >  1:1084956/1‑139 (MQ=255)                                              gTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccacc                                                                                   <  2:1084956/139‑1 (MQ=255)                                                 aCATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGCCGATCATGGCGAAAGTCCGCCCTTATATTGGcaccaccacca                                                                               >  2:1317524/1‑140 (MQ=255)                                                           aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGt                                                                     <  2:767036/140‑1 (MQ=255)                                                           aCCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGt                                                                     >  2:913167/1‑140 (MQ=255)                                                             ccGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCt                                                                            >  2:1177345/1‑131 (MQ=255)                                                             ccGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCt                                                                            <  1:1177345/131‑1 (MQ=255)                                                                     gCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAggcg                                                           >  2:610335/1‑140 (MQ=255)                                                                      cGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAggcgg                                                          >  2:877857/1‑140 (MQ=255)                                                                                tACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCgg                                                <  1:668430/140‑1 (MQ=255)                                                                                     ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                           <  2:41691/140‑1 (MQ=255)                                                                                     ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                           >  1:41691/1‑140 (MQ=255)                                                                                     ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                           >  1:341257/1‑140 (MQ=255)                                                                                     ttCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCt                                           >  2:857522/1‑140 (MQ=255)                                                                                      tCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCtt                                          >  1:806226/1‑140 (MQ=255)                                                                                          gTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATg                                      >  1:255638/1‑140 (MQ=255)                                                                                               tGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCa                                 <  2:255638/140‑1 (MQ=255)                                                                                                     gAAGCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTgc                           <  2:1402560/140‑1 (MQ=255)                                                                                                        gCTGTATGCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCt                        <  1:995446/140‑1 (MQ=255)                                                                                                               gCAGGCTGGCACAACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCtt                 <  1:913167/140‑1 (MQ=255)                                                                                                                         acaACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTg       <  1:362843/140‑1 (MQ=255)                                                                                                                          caACTTGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGc      <  1:877857/140‑1 (MQ=255)                                                                                                                              ttGCTGGATAT‑GGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCctg  <  1:857522/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GTATCGTCGACGGCAGCGGCCCGCTAATTAAAGCCATTTCCAGCGGTGACATGACGTTACCCGATCTGGCGTGGCTTAATACCATTCCGGTGATTGGCGCGAAGCTGTATGCAGGCTGGCACAACTTGCTGGATATGGGGGGGACGGCGATCATGGCGAAAGTCCGCCCTTATATTGGCACCACCACCACCTGGTTCGTTGGGCAGGCGGCGCATATCGGGCGCTTTATGGTGCATTGTGCGCTGATGCTTCTCTTCAGTGCCCTG  >  NC_000913/1769336‑1769601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑