Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,937	(A)6→5	coding (1120/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,932	0	A	.	100.0%	343.1 / NA	79	coding (1115/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (50/29);  total (50/29)

TCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACT  >  NC_000913/2602796‑2603067                                                                                                                                         |                                                                                                                                        tCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGaaaa                                                                                                                                      <  2:1241958/140‑1 (MQ=255)          gACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCa                                                                                                                            <  1:517255/140‑1 (MQ=255)          gACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCa                                                                                                                            <  2:1170504/140‑1 (MQ=255)           aCCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCAc                                                                                                                           >  2:781626/1‑140 (MQ=255)           aCCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTACATACCCACGACATGG‑AAAAATGGGGGCAc                                                                                                                           >  2:382953/1‑140 (MQ=255)               gctgctCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGc                                                                                                                       <  1:781626/140‑1 (MQ=255)                     cACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACg                                                                                                                 <  1:1235151/140‑1 (MQ=255)                      aCCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACgg                                                                                                                <  1:1361443/140‑1 (MQ=255)                      aCCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCCGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACgg                                                                                                                >  1:108161/1‑140 (MQ=255)                             ttGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGt                                                                                                         >  2:875579/1‑140 (MQ=255)                                gACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGa                                                                                                      <  2:204650/140‑1 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGcc                                                                                                 >  2:1384359/1‑140 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGcc                                                                                                 >  2:943710/1‑140 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGcc                                                                                                 >  1:456921/1‑140 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGc                                                                                                  >  2:708628/1‑139 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGc                                                                                                  >  2:785349/1‑139 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGc                                                                                                  <  1:708628/139‑1 (MQ=255)                                     cTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGc                                                                                                  <  1:785349/139‑1 (MQ=255)                                      tCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCg                                                                                                >  2:575874/1‑140 (MQ=255)                                         gcgcACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCgcag                                                                                             <  2:456921/140‑1 (MQ=255)                                         gcgcACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCgcag                                                                                             <  1:875579/140‑1 (MQ=255)                                         gcgcACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCgcag                                                                                             <  1:564007/140‑1 (MQ=255)                                          cgcACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCgcagc                                                                                            >  2:1025723/1‑140 (MQ=255)                                          cgcACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCgcagc                                                                                            >  2:704480/1‑140 (MQ=255)                                               tGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGACGTGGACAGCCGCAGCATGcc                                                                                       >  2:1293511/1‑140 (MQ=255)                                               tGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATGTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGcc                                                                                       >  2:971948/1‑140 (MQ=255)                                                    cATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGAtt                                                                                  >  2:444275/1‑140 (MQ=255)                                                         gCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTg                                                                             >  2:293966/1‑140 (MQ=255)                                                         gCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTg                                                                             >  2:97247/1‑140 (MQ=255)                                                         gCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTg                                                                             >  1:924952/1‑140 (MQ=255)                                                          cTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGc                                                                            <  2:146675/140‑1 (MQ=255)                                                           tCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGtt                                                                              >  1:975622/1‑137 (MQ=255)                                                           tCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGtt                                                                              <  2:975622/137‑1 (MQ=255)                                                           tCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGcc                                                                           >  2:18/1‑140 (MQ=255)                                                           tCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGcc                                                                           >  1:31079/1‑140 (MQ=255)                                                              aCCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTcg                                                                        <  2:991395/140‑1 (MQ=255)                                                              aCCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTcg                                                                        <  1:1373712/140‑1 (MQ=255)                                                                  tGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGat                                                                    >  2:889558/1‑140 (MQ=255)                                                                  tGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGat                                                                    <  1:772421/140‑1 (MQ=255)                                                                  tGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGat                                                                    >  1:1292345/1‑140 (MQ=255)                                                                    cgcTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGatat                                                                  >  1:381140/1‑140 (MQ=255)                                                                         ttCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGcc                                                             >  1:1031980/1‑140 (MQ=255)                                                                         ttCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGcc                                                             >  2:688879/1‑140 (MQ=255)                                                                         ttCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGcc                                                             >  1:1367049/1‑140 (MQ=255)                                                                         ttCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGcc                                                             >  2:716978/1‑140 (MQ=255)                                                                             aaGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATtc                                                         <  1:1229550/140‑1 (MQ=255)                                                                              aGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTcg                                                                        <  2:620819/124‑1 (MQ=255)                                                                              aGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTcg                                                                        >  1:620819/1‑124 (MQ=255)                                                                                 gctgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATtcctcc                                                     >  2:333968/1‑140 (MQ=255)                                                                                  ctgctATTTCTCCGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCg                                                    <  2:108161/140‑1 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  2:39222/1‑140 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  2:62499/1‑140 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  2:446146/1‑140 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  1:1420216/1‑140 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  2:306936/1‑140 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  1:1299808/1‑140 (MQ=255)                                                                                   tgctATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGc                                                   >  2:92467/1‑140 (MQ=255)                                                                                      tATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGa                                                <  1:575874/140‑1 (MQ=255)                                                                                      tATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGa                                                <  2:1367049/140‑1 (MQ=255)                                                                                      tATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGa                                                <  1:1193207/140‑1 (MQ=255)                                                                                               gcgcGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCATGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTa                                       >  2:1315578/1‑140 (MQ=255)                                                                                                  cgGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCa                                    >  1:1013393/1‑140 (MQ=255)                                                                                                  cgGGAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCa                                    >  2:376165/1‑140 (MQ=255)                                                                                                    ggAGCGATTATTTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGc                                  <  1:688879/140‑1 (MQ=255)                                                                                                          attattTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATgg                            >  2:265917/1‑140 (MQ=255)                                                                                                             attTCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTac                         >  1:778547/1‑140 (MQ=255)                                                                                                               ttCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGc                                  <  2:1219005/129‑1 (MQ=255)                                                                                                               ttCGCGTTTGCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGc                                  >  1:1219005/1‑129 (MQ=255)                                                                                                                        gCATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTc              <  2:1292345/140‑1 (MQ=255)                                                                                                                         cATACCCACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCg             <  1:293966/140‑1 (MQ=255)                                                                                                                               cACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTtc       <  1:889558/140‑1 (MQ=255)                                                                                                                               cACGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTtc       >  1:1266991/1‑140 (MQ=255)                                                                                                                                aCGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTtct      >  1:582293/1‑140 (MQ=255)                                                                                                                                 cGACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTtctc     >  2:161307/1‑140 (MQ=255)                                                                                                                                  gACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGt         <  1:957575/135‑1 (MQ=255)                                                                                                                                  gACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGt         >  2:957575/1‑135 (MQ=255)                                                                                                                                  gACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCa    >  1:570383/1‑140 (MQ=255)                                                                                                                                  gACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCa    >  1:1138137/1‑140 (MQ=255)                                                                                                                                  gACATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCa    >  1:352419/1‑140 (MQ=255)                                                                                                                                    cATGG‑AAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACt  <  2:1013393/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCACTGCACGACCCGCTGCTCACCGTGGTTGGACTGCTCGGCGCACTGTTTCATCTGCTCAACCATGCGCTGTTCAAAGGGCTGCTATTTCTCGGCGCGGGAGCGATTATTTCGCGTTTGCATACCCACGACATGGAAAAAATGGGGGCACTAGCGAAACGGATGCCGTGGACAGCCGCAGCATGCCTGATTGGTTGCCTCGCGATATCAGCCATTCCTCCGCTGAATGGTTTTATCAGCGAATGGTACACCTGGCAGTCGCTGTTCTCACT  >  NC_000913/2602796‑2603067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑