Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	601,940	(A)6→7	intergenic (+83/‑19)	cusA → / → pheP	copper/silver export system RND permease/phenylalanine:H(+) symporter PheP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	601,934	1	.	A	100.0%	293.0 / NA	81	intergenic (+77/‑25)	cusA/pheP	copper/silver export system RND permease/phenylalanine:H(+) symporter PheP
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (46/35);  total (46/35)

TTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑AAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCG  >  NC_000913/601798‑602064                                                                                                                                          |                                                                                                                                   tttATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaa                                                                                                                                 <  1:323607/140‑1 (MQ=255) tttATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaa                                                                                                                                 <  2:628797/140‑1 (MQ=255)      tatCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaag                                                                                                                            <  1:645675/140‑3 (MQ=255)       atCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaaga                                                                                                                           <  1:171135/140‑4 (MQ=255)       atCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaaga                                                                                                                           <  1:732354/140‑4 (MQ=255)        tCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaagac                                                                                                                          >  2:1054372/1‑136 (MQ=255)         cccGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaagaca                                                                                                                         <  1:496518/140‑6 (MQ=255)         cccGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaagaca                                                                                                                         <  2:1330772/140‑6 (MQ=255)         cccGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaagaca                                                                                                                         <  2:1285586/140‑6 (MQ=255)         cccGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑aaaaaaagaca                                                                                                                         <  2:969816/140‑6 (MQ=255)           cggcggCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGacaca                                                                                                                        >  2:613938/1‑140 (MQ=255)             gcggcgTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGACACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGAcacaca                                                                                                                      >  2:1318403/1‑140 (MQ=255)                  gTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGa                                                                                                                 <  1:532237/140‑1 (MQ=255)                   tataAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGaa                                                                                                                >  2:1007876/1‑140 (MQ=255)                          tGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTg                                                                                                         >  1:450613/1‑140 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAgggg                                                                                                                  <  1:38980/128‑1 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAgggg                                                                                                                  >  2:38980/1‑128 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGaaa                                                                                                      >  2:829958/1‑140 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGaaa                                                                                                      <  1:249956/140‑1 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGaaa                                                                                                      <  1:1245654/140‑1 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGaaa                                                                                                      >  1:911185/1‑140 (MQ=255)                             tgtgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGaaa                                                                                                      >  2:436244/1‑140 (MQ=255)                               tgGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGaaaaa                                                                                                    <  1:943535/140‑1 (MQ=255)                                  cTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAAcgc                                                                                                 >  1:775005/1‑140 (MQ=255)                                     cACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTc                                                                                              <  1:1012661/140‑1 (MQ=255)                                      aCCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCa                                                                                             >  1:418650/1‑140 (MQ=255)                                      aCCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCa                                                                                             >  1:1062269/1‑140 (MQ=255)                                       ccGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGTTGCCCCTCAACAAAAAAAGACACACAGGGGAATGGCGTGAAAAACGCGTCaa                                                                                            >  1:1330281/1‑140 (MQ=255)                                       ccGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCaa                                                                                            >  2:686862/1‑140 (MQ=255)                                        cGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAAc                                                                                           <  1:103366/140‑1 (MQ=255)                                          aCATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGTTGCCCCTCAACAAAAAAAGACACACAGGGGAATGGCGTGAAAAACGCGTCAACCg                                                                                         <  2:1330281/140‑1 (MQ=255)                                          aCATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCg                                                                                         <  2:911185/140‑1 (MQ=255)                                           cATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGt                                                                                        <  2:1211436/140‑1 (MQ=255)                                            aTCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTa                                                                                       <  2:775005/140‑1 (MQ=255)                                             tCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTAt                                                                                      <  2:1359928/140‑1 (MQ=255)                                                 gTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGCCAACCGTATCGGa                                                                                  <  1:829958/140‑1 (MQ=255)                                                     ggAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGAt                                                                              >  2:603407/1‑140 (MQ=255)                                                      gAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATa                                                                             >  1:114755/1‑140 (MQ=255)                                                                          cGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCg                                                         <  1:1054372/140‑1 (MQ=255)                                                                           gTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGa                                                        <  1:1007876/140‑1 (MQ=255)                                                                            tttAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGAc                                                       <  2:975018/140‑1 (MQ=255)                                                                             ttAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACg                                                      <  2:398340/140‑1 (MQ=255)                                                                                    tgtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATc                                               <  1:613938/140‑1 (MQ=255)                                                                                    tgtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATc                                               >  2:216427/1‑140 (MQ=255)                                                                                    tgtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATc                                               >  1:737038/1‑140 (MQ=255)                                                                                     gtgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcg                                              >  1:752201/1‑140 (MQ=255)                                                                                      tgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGcc                                                          >  2:1082780/1‑127 (MQ=255)                                                                                      tgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGcc                                                          <  1:1082780/127‑1 (MQ=255)                                                                                      tgGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgc                                             >  1:1987/1‑140 (MQ=255)                                                                                       ggATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgcg                                            <  2:752201/140‑1 (MQ=255)                                                                                        gATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCgg                                           <  1:1318403/140‑1 (MQ=255)                                                                                           tgtgTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGAtt                                        >  1:1387079/1‑140 (MQ=255)                                                                                            gtgtCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTa                                       <  2:450613/140‑1 (MQ=255)                                                                                               tCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgcg                                            <  2:1247678/132‑1 (MQ=255)                                                                                               tCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATcgcg                                            >  1:1247678/1‑132 (MQ=255)                                                                                               tCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACAt                                    >  1:1152484/1‑140 (MQ=255)                                                                                                 ttGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATaa                                  <  1:603407/140‑1 (MQ=255)                                                                                                 ttGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATaa                                  <  1:436244/140‑1 (MQ=255)                                                                                                  tGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCCCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAAc                                 >  2:898344/1‑140 (MQ=255)                                                                                                   gCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAAcc                                >  1:1263042/1‑140 (MQ=255)                                                                                                   gCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAAcc                                >  1:1055344/1‑140 (MQ=255)                                                                                                   gCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAAcc                                >  1:26619/1‑140 (MQ=255)                                                                                                    cgacgaTGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCg                               <  2:647099/140‑1 (MQ=255)                                                                                                       cgaTGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCa                            >  1:1203354/1‑140 (MQ=255)                                                                                                         aTGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCg                               <  1:582337/135‑1 (MQ=255)                                                                                                         aTGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCg                               >  2:582337/1‑135 (MQ=255)                                                                                                          tGGGCACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCatat                         <  1:1154313/140‑1 (MQ=255)                                                                                                              cACTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAGGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCaa                     >  2:1306405/1‑140 (MQ=255)                                                                                                               aCTAAATGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAAc                    <  2:1263042/140‑1 (MQ=255)                                                                                                                  aaaTGTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGa                 <  1:65872/140‑1 (MQ=255)                                                                                                                      gTTAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGc             >  1:82805/1‑140 (MQ=255)                                                                                                                       ttAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCg            <  2:1203354/140‑1 (MQ=255)                                                                                                                        tAAAAGGTGCCTCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGt           >  2:1213447/1‑140 (MQ=255)                                                                                                                        tAAAAGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCGTATTCAACTGATTGCGt           >  2:450468/1‑140 (MQ=255)                                                                                                                           aaGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTgg        >  1:1062777/1‑140 (MQ=255)                                                                                                                            aGGTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTggg       <  2:1387079/140‑1 (MQ=255)                                                                                                                             ggTGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGCTGGGt      >  1:687909/1‑140 (MQ=255)                                                                                                                               tGCCCCTCAACAAAAAAATACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTgg    >  2:595358/1‑140 (MQ=255)                                                                                                                               tGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTgg    >  1:329179/1‑140 (MQ=255)                                                                                                                               tGCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTgg    >  1:409361/1‑140 (MQ=255)                                                                                                                                gCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgc   <  1:1306405/140‑1 (MQ=255)                                                                                                                                gCCCCTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgc   >  2:1055114/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTTGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  >  2:989207/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGa                 <  1:450508/125‑1 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGa                 >  2:450508/1‑125 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  >  1:819652/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  >  2:1002834/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  >  2:605107/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  >  1:1399474/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  >  2:214341/1‑140 (MQ=255)                                                                                                                                 ccccTCAACAAAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGgcg  <  2:404630/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                   TTTATTATCCCGGCGGCGTATAAGCTGATGTGGCTGCACCGACATCGGGTACGGAAATAAAAGCAGGATACCCCGTTTAACCGTGTGGATTGTGTCTTGCGACGATGGGCACTAAATGTTAAAAGGTGCCCCTCAAC‑AAAAAAGACACACAGGGGAAAGGCGTGAAAAACGCGTCAACCGTATCGGAAGATACTGCGTCGAATCAAGAGCCGACGCTTCATCGCGGATTACATAACCGTCATATTCAACTGATTGCGTTGGGTGGCG  >  NC_000913/601798‑602064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑