Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,793	(T)7→8	intergenic (‑141/‑93)	serX ← / → ghrA	tRNA‑Ser/glyoxylate/hydroxypyruvate reductase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,786	1	.	T	100.0%	258.2 / NA	73	intergenic (‑134/‑100)	serX/ghrA	tRNA‑Ser/glyoxylate/hydroxypyruvate reductase A
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (31/42);  total (31/42)

CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑TTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCAACGTTCGA  >  NC_000913/1097649‑1097917                                                                                                                                           |                                                                                                                                    cACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑tt                                                                                                                                   >  1:62673/1‑140 (MQ=255) cACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑tt                                                                                                                                   >  2:842852/1‑140 (MQ=255) cACCAAATTGTCGTTCCTGTCTTCCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑tt                                                                                                                                   >  1:1405360/1‑140 (MQ=255)  aCCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑ttt                                                                                                                                  >  2:89621/1‑140 (MQ=255)   ccAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑tttt                                                                                                                                 >  2:588811/1‑140 (MQ=255)         tGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑ttttttttgc                                                                                                                           >  2:821276/1‑137 (MQ=255)         tGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑ttttttttgc                                                                                                                           >  1:815775/1‑137 (MQ=255)           tCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑ttttttttgcca                                                                                                                         >  1:475222/1‑135 (MQ=255)              ttCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCg                                                                                                                       >  1:65399/1‑140 (MQ=255)               tCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGa                                                                                                                      <  2:1226612/140‑1 (MQ=255)                  tGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCa                                                                                                                   <  1:19745/140‑1 (MQ=255)                   gTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCAc                                                                                                                  >  2:894839/1‑140 (MQ=255)                         cTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGAttt                                                                                                            >  1:106810/1‑140 (MQ=255)                            gAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGtt                                                                                                         >  2:403966/1‑140 (MQ=255)                             aaCGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTa                                                                                                        <  2:1347236/140‑1 (MQ=255)                              aCGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTAt                                                                                                       >  2:1183922/1‑140 (MQ=255)                                gggCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTACAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGc                                                                                                     >  2:571016/1‑140 (MQ=255)                                 ggCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCt                                                                                                    >  1:232417/1‑140 (MQ=255)                                 ggCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCt                                                                                                    >  1:793021/1‑140 (MQ=255)                                  gcgcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGGAATCTGCTTTTTTTTGCCACCGACCACGGAtt                                                                                                             >  2:1113119/1‑130 (MQ=255)                                  gcgcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGGAATCTGCTTTTTTTTGCCACCGACCACGGAtt                                                                                                             <  1:1113119/130‑1 (MQ=255)                                   cgcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCtgg                                                                                                  >  1:563810/1‑140 (MQ=255)                                   cgcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCtg                                                                                                   <  1:1228070/139‑1 (MQ=255)                                   cgcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCtg                                                                                                   >  2:1228070/1‑139 (MQ=255)                                    gcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCtggt                                                                                                 <  2:106367/140‑1 (MQ=255)                                    gcTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCtggt                                                                                                 <  1:842852/140‑1 (MQ=255)                                       aaTTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGc                                                                                              <  1:1225353/140‑1 (MQ=255)                                       aaTTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGc                                                                                              <  2:62673/140‑1 (MQ=255)                                       aaTTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGc                                                                                              <  2:1405360/140‑1 (MQ=255)                                         tttAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCt                                                                                            <  1:1419009/140‑1 (MQ=255)                                           tAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCttt                                                                                          >  2:623795/1‑140 (MQ=255)                                            aGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTg                                                                                         <  1:1339281/140‑1 (MQ=255)                                              ggAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTa                                                                                       <  1:588811/140‑1 (MQ=255)                                              ggAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTa                                                                                       >  2:999974/1‑140 (MQ=255)                                                aaaTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGa                                                                                     <  1:403966/140‑1 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTa                                                                                       <  1:381652/136‑1 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTa                                                                                       >  2:381652/1‑136 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   <  1:1269735/140‑1 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   >  1:277177/1‑140 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   >  1:1399543/1‑140 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   <  2:563810/140‑1 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   <  2:538308/140‑1 (MQ=255)                                                  aTCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   <  2:214606/140‑1 (MQ=255)                                                    cATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCAt                                                                                 <  2:65399/140‑1 (MQ=255)                                                       gACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAAc                                                                              >  2:297883/1‑140 (MQ=255)                                                            gAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATaa                                                                         >  2:723690/1‑140 (MQ=255)                                                             aGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAg                                                                        <  2:106810/140‑1 (MQ=255)                                                               gTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTg                                                                      >  1:607198/1‑140 (MQ=255)                                                               gTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTg                                                                      >  2:786557/1‑140 (MQ=255)                                                                  aacaaACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   <  1:798112/124‑1 (MQ=255)                                                                  aacaaACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATc                                                                                   >  2:798112/1‑124 (MQ=255)                                                                  aacaaACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGa                                                                   >  1:700662/1‑140 (MQ=255)                                                                                aaaaaTCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGcaca                                                     <  2:793021/140‑1 (MQ=255)                                                                                aaaaaTCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGcaca                                                     <  2:232417/140‑1 (MQ=255)                                                                                 aaaaTCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACaa                                                    <  2:475222/140‑1 (MQ=255)                                                                                             ttATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGa                                        <  2:277177/140‑1 (MQ=255)                                                                                             ttATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGa                                        <  1:786557/140‑1 (MQ=255)                                                                                              tATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAg                                       <  1:723690/140‑1 (MQ=255)                                                                                              tATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAg                                       <  2:607198/140‑1 (MQ=255)                                                                                               aTTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGt                                      <  1:203025/140‑1 (MQ=255)                                                                                                     aCTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGa                                        >  2:244862/1‑132 (MQ=255)                                                                                                     aCTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGa                                        <  1:244862/132‑1 (MQ=255)                                                                                                     aCTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTAt                                >  1:19099/1‑140 (MQ=255)                                                                                                       ttCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAg                                       <  1:950619/131‑1 (MQ=255)                                                                                                       ttCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAg                                       >  2:950619/1‑131 (MQ=255)                                                                                                          aatcaatGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGata                           <  1:999974/140‑1 (MQ=255)                                                                                                           atcaatGTGTGGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGatat                          <  2:1196968/140‑1 (MQ=255)                                                                                                                tgtgtgGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCt                     >  1:504026/1‑140 (MQ=255)                                                                                                                 gtgtgGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGatat                          >  1:1308950/1‑134 (MQ=255)                                                                                                                 gtgtgGTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGatat                          <  2:1308950/134‑1 (MQ=255)                                                                                                                     ggTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTAt                <  2:378627/140‑1 (MQ=255)                                                                                                                      gTTTTAATAAGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATc               <  2:1399543/140‑1 (MQ=255)                                                                                                                          taataaGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCAccc           >  1:998344/1‑140 (MQ=255)                                                                                                                          taataaGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCAccc           >  2:1231277/1‑140 (MQ=255)                                                                                                                           aataaGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCa          <  2:504026/140‑1 (MQ=255)                                                                                                                           aataaGCGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCa          <  1:89621/140‑1 (MQ=255)                                                                                                                                gCGAAATCTGCTTTTTTTTGCCACCGACCACGGATCTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCAACGtt     <  2:980045/140‑1 (MQ=255)                                                                                                                                 cGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCAACGTTc    >  2:442785/1‑140 (MQ=255)                                                                                                                                 cGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGACATCATCTTTTATCAcc            <  1:427750/132‑1 (MQ=255)                                                                                                                                 cGAAATCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGACATCATCTTTTATCAcc            >  2:427750/1‑132 (MQ=255)                                                                                                                                   aaaTCTGCTTTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCAACGTTCGa  <  1:821276/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                    CACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGC‑TTTTTTTGCCACCGACCACGGATTTGTTATGCTGGTGGCCTTTGTAGATCATAACGATAAGTGCGAATAAATTTCGCACAACGCTTTTCGGGAGTCAGTATGGATATCATCTTTTATCACCCAACGTTCGA  >  NC_000913/1097649‑1097917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑