Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,194,558	(A)5→6	intergenic (+33/+163)	yqiK → / ← sibD	protein YqiK/small RNA SibD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,194,553	1	.	A	100.0%	255.9 / NA	72	intergenic (+28/+168)	yqiK/sibD	protein YqiK/small RNA SibD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (36/36);  total (36/36)

CACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑C‑AAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCAT  >  NC_000913/3194416‑3194687                                                                                                                                            |                                                                                                                                       cACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑C‑aa                                                                                                                                      >  2:409027/1‑140 (MQ=255)     gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑C‑aaaaaa                                                                                                                                  >  1:484132/1‑139 (MQ=255)      cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑C‑aaaaaag                                                                                                                                 >  1:740952/1‑138 (MQ=255)           tGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACC‑CAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCgggg                                                                                                                            >  1:843093/1‑140 (MQ=255)             aCTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGa                                                                                                                           <  1:1139261/140‑1 (MQ=255)             aCTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGa                                                                                                                           <  2:835457/140‑1 (MQ=255)                 cTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGa                                                                                                                           <  2:1284481/136‑1 (MQ=255)                 cTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGa                                                                                                                           >  1:1284481/1‑136 (MQ=255)                 cTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGAAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGACGGGGAtttt                                                                                                                       >  1:1011784/1‑140 (MQ=255)                   tGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGAtttttt                                                                                                                     >  2:1240671/1‑140 (MQ=255)                   tGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGAtttttt                                                                                                                     >  1:1365310/1‑140 (MQ=255)                    gCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTta                                                                                                                    <  1:641965/140‑1 (MQ=255)                         aTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCt                                                                                                               >  1:228800/1‑140 (MQ=255)                           gAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGc                                                                                                             >  1:841901/1‑140 (MQ=255)                            aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCg                                                                                                            <  1:1121492/140‑1 (MQ=255)                            aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCg                                                                                                            >  2:850644/1‑140 (MQ=255)                            aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCg                                                                                                            <  2:969642/140‑1 (MQ=255)                              aTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGtt                                                                                                          >  2:1189661/1‑140 (MQ=255)                                       tCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaa                                                                                                 <  2:1344035/140‑1 (MQ=255)                                         aGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                                                                                               <  2:740952/140‑1 (MQ=255)                                          ggcggcTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGt                                                                                              <  1:850644/140‑1 (MQ=255)                                             ggcTCACTGGCGGCATTGACTTCACCCTTAACC‑CAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaa                                                                                          <  2:843093/140‑1 (MQ=255)                                                   cTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt                                                                                     <  2:484132/140‑1 (MQ=255)                                                       cggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg                                                                                 <  1:1189661/140‑1 (MQ=255)                                                         gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt                                                                               >  1:1359926/1‑140 (MQ=255)                                                          cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg                                                                              <  1:1035038/140‑1 (MQ=255)                                                          cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgttg                                                                              <  1:451125/140‑1 (MQ=255)                                                          cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGT‑‑AAATGATCAGgttgtt                                                                            <  2:228800/140‑1 (MQ=38)                                                              gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc                                                                          <  2:1365310/140‑1 (MQ=255)                                                              gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgc                                                                          <  2:1409045/140‑1 (MQ=255)                                                                 ttCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgcagc                                                                       >  2:132008/1‑140 (MQ=255)                                                                    aCCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCAtt                                                                    >  2:1352212/1‑140 (MQ=255)                                                                        ttAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGc                                                                >  2:778515/1‑140 (MQ=255)                                                                        ttAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGc                                                                >  2:376856/1‑140 (MQ=255)                                                                         tAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCg                                                               >  2:596867/1‑140 (MQ=255)                                                                           aCCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTg                                                             <  1:1007145/140‑1 (MQ=255)                                                                           aCCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTg                                                             >  2:501984/1‑140 (MQ=255)                                                                            ccTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGa                                                            <  2:217715/140‑1 (MQ=255)                                                                            ccTCAACAACTCCCGTCGAAG‑AAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGAc                                                           <  1:858728/140‑1 (MQ=255)                                                                                aacaacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgca                                                                         <  1:256303/123‑1 (MQ=255)                                                                                aacaacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTgca                                                                         >  2:256303/1‑123 (MQ=255)                                                                                aacaacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGc                                                        >  2:164119/1‑140 (MQ=255)                                                                                  caacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCT‑AAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCg                                                     >  1:1097293/1‑140 (MQ=255)                                                                                   aacTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCg                                                     <  2:219527/140‑1 (MQ=255)                                                                                    acTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCgg                                                    >  1:1234559/1‑140 (MQ=255)                                                                                     cTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg                                                   >  1:648815/1‑140 (MQ=255)                                                                                     cTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCggg                                                   <  1:596867/140‑1 (MQ=255)                                                                                               agaaAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa                                         <  2:1234559/140‑1 (MQ=255)                                                                                                    aaGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCa                                    <  1:1352212/140‑1 (MQ=255)                                                                                                          agTAAACCCTCTTCCAGGAATAATCCCTGGCCAA‑AAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCagag                              <  2:1011784/140‑1 (MQ=255)                                                                                                           gTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa                                         <  1:788675/128‑1 (MQ=255)                                                                                                           gTAAACCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCaaaa                                         >  2:788675/1‑128 (MQ=255)                                                                                                               aCCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc                         <  1:778515/140‑1 (MQ=255)                                                                                                               aCCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc                         <  1:376856/140‑1 (MQ=255)                                                                                                               aCCCTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGc                         <  2:568530/140‑1 (MQ=255)                                                                                                                cccTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAATTGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCa                        <  1:1240671/140‑1 (MQ=255)                                                                                                                 ccTCTTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCAt                       <  1:164119/140‑1 (MQ=255)                                                                                                                    ctTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGc                    >  2:123831/1‑140 (MQ=255)                                                                                                                    ctTCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGc                    >  2:266866/1‑140 (MQ=255)                                                                                                                      tCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGTAAGGCAAAACTGCATCAGAGAAAGCATTGCGt                  >  2:777471/1‑140 (MQ=255)                                                                                                                      tCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGt                  >  2:436605/1‑140 (MQ=255)                                                                                                                      tCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGt                  >  1:234698/1‑140 (MQ=255)                                                                                                                      tCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGt                  >  2:122656/1‑140 (MQ=255)                                                                                                                      tCCAGGAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGt                  >  1:1231835/1‑140 (MQ=255)                                                                                                                          ggAATAATCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTgggg              >  1:332631/1‑140 (MQ=255)                                                                                                                            aataatCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTc            <  2:1359926/140‑1 (MQ=255)                                                                                                                               aatCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTgg         >  2:1024555/1‑140 (MQ=255)                                                                                                                               aatCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTgg         >  2:614390/1‑140 (MQ=255)                                                                                                                                 tCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg       >  2:82263/1‑140 (MQ=255)                                                                                                                                 tCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg       <  1:1002762/140‑1 (MQ=255)                                                                                                                                 tCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg       <  1:266866/140‑1 (MQ=255)                                                                                                                                 tCCCTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGgcg       >  2:1411624/1‑140 (MQ=255)                                                                                                                                  cccTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCT‑AAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGCGGGGTCCGGCGCt     <  2:1097293/140‑1 (MQ=14)                                                                                                                                   ccTGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCt     <  1:123831/140‑1 (MQ=255)                                                                                                                                     tGGCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtca   >  2:1047196/1‑140 (MQ=255)                                                                                                                                      ggCC‑CAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCtcat  >  2:1325227/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                       CACCGCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCC‑C‑AAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTTGTTGCAGCATTTTGCGTGACGGCTCGGGGAAGGCAAAACTGCATCAGAGAAAGCATTGCGTGGGGTCTGGCGCTCAT  >  NC_000913/3194416‑3194687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑