Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,129 (T)6→7 coding (119/333 nt) ygjH ← putative tRNA‑binding protein YgjH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,1231.T98.9% 325.9 / ‑7.1 93T42K (ACG→AAG) ygjHputative tRNA‑binding protein YgjH
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base T (45/47);  minor base C (0/1);  total (45/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑TTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTC  >  NC_000913/3220986‑3221256
                                                                                                                                          |                                                                                                                                     
tttCCGCACACAACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tt                                                                                                                                     >  1:533838/1‑140 (MQ=255)
tttCCGCACACAACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tt                                                                                                                                     >  1:268997/1‑140 (MQ=255)
    cGCACACAACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttt                                                                                                                                 <  1:901703/140‑1 (MQ=255)
        cacaACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttttgcc                                                                                                                             <  2:530901/140‑5 (MQ=255)
        cacaACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttttgcc                                                                                                                             <  1:501739/140‑5 (MQ=255)
        cacaACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttttgcc                                                                                                                             <  1:1173435/140‑5 (MQ=255)
        cacaACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttttgcc                                                                                                                             <  2:799718/140‑5 (MQ=255)
        cacaACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTAACGGTTTGCAGCG‑tttttttgcc                                                                                                                             <  2:386844/140‑5 (MQ=255)
         acaacaGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttttg                                                                                                                               <  2:756130/137‑3 (MQ=255)
         acaacaGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑tttttttg                                                                                                                               >  1:756130/1‑135 (MQ=255)
          cgacaGCATGCAGTCCGACGTTTCCCCACGCATTTTCGCTTTTTGCAGATTGCATAAGCCCCCCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGcccc                                                                                                                            <  2:48555/138‑1 (MQ=255)
          caacaGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGcccc                                                                                                                            <  2:1360418/140‑1 (MQ=255)
              aGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACAt                                                                                                                        >  1:1111456/1‑140 (MQ=255)
               gcatgcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATc                                                                                                                       <  2:268997/140‑1 (MQ=255)
               gcatgcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATc                                                                                                                       <  2:73346/140‑1 (MQ=255)
                 atgcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCaa                                                                                                                     >  1:1232796/1‑140 (MQ=255)
                 atgcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCaa                                                                                                                     <  1:96550/140‑1 (MQ=255)
                   gcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAAcc                                                                                                                   >  2:279447/1‑140 (MQ=255)
                   gcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAAcc                                                                                                                   >  2:1171234/1‑140 (MQ=255)
                   gcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAAcc                                                                                                                   >  2:202886/1‑140 (MQ=255)
                   gcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAAcc                                                                                                                   >  2:160930/1‑140 (MQ=255)
                   gcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGCTTGCAGCGTTTTTTTGCCCCACATCAAcc                                                                                                                   >  1:454991/1‑140 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCt                                                                                                                  <  2:618858/140‑1 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCt                                                                                                                  <  2:1232796/140‑1 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCt                                                                                                                  <  1:1256333/140‑1 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCt                                                                                                                  <  2:1103249/140‑1 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCt                                                                                                                  <  1:169630/140‑1 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGCTTTTTTGCCCCACATCAACCt                                                                                                                  <  2:1006920/140‑1 (MQ=255)
                    catTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTGCCCCATCAGCTCTTCTTCGCTGTAGTTCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCt                                                                                                                  <  1:1338647/140‑1 (MQ=255)
                       tCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTa                                                                                                               >  1:1385823/1‑140 (MQ=255)
                         cGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTAc                                                                                                              >  2:899536/1‑139 (MQ=255)
                         cGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTAc                                                                                                              <  1:899536/139‑1 (MQ=255)
                          gACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGa                                                                                                            >  1:1253151/1‑140 (MQ=255)
                              tttCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTa                                                                                                        >  2:684085/1‑140 (MQ=255)
                                tCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACa                                                                                                      >  1:661203/1‑140 (MQ=255)
                                         aTTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCgg                                                                                             <  1:1171234/140‑1 (MQ=255)
                                         aTTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCgg                                                                                             <  2:781699/140‑1 (MQ=255)
                                                 tttttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCa                                                                                     <  1:892330/140‑1 (MQ=255)
                                                 tttttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCa                                                                                     <  2:654846/140‑1 (MQ=255)
                                                   tttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATg                                                                                   >  1:1044111/1‑140 (MQ=255)
                                                   tttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATg                                                                                   <  1:1144088/140‑1 (MQ=255)
                                                     tGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGc                                                                                 >  1:783098/1‑140 (MQ=255)
                                                           ttacATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCa                                                                           <  2:872070/137‑1 (MQ=255)
                                                           ttGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCa                                                                           <  2:1044111/140‑1 (MQ=255)
                                                           ttGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCa                                                                           <  2:1253151/140‑1 (MQ=255)
                                                           ttGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCa                                                                           <  2:920840/140‑1 (MQ=255)
                                                               aTAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc                                                                       <  2:901518/140‑1 (MQ=255)
                                                                   gACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCcaca                                                                   >  1:379460/1‑140 (MQ=255)
                                                                    accaccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACaa                                                                  >  1:1007649/1‑140 (MQ=255)
                                                                      caccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCcac                                                                    <  2:512472/136‑1 (MQ=255)
                                                                      caccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCcac                                                                    >  1:512472/1‑136 (MQ=255)
                                                                      caccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATc                                                                >  2:610620/1‑140 (MQ=255)
                                                                      caccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATAAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATc                                                                >  2:808290/1‑140 (MQ=255)
                                                                        ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt                                                              <  2:783098/140‑1 (MQ=255)
                                                                        ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt                                                              >  2:808268/1‑140 (MQ=255)
                                                                        ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt                                                              >  2:1101720/1‑140 (MQ=255)
                                                                        ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt                                                              >  1:373182/1‑140 (MQ=255)
                                                                              ttttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAc                                                        >  1:385598/1‑140 (MQ=255)
                                                                              ttttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAc                                                        >  1:765279/1‑140 (MQ=255)
                                                                                ttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcgc                                                      <  2:379460/140‑1 (MQ=255)
                                                                                ttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcgc                                                      <  1:678328/140‑1 (MQ=255)
                                                                                  ccccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACgcgc                                                    <  2:1385823/140‑1 (MQ=255)
                                                                                   cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGa                                                         <  2:1033387/134‑1 (MQ=255)
                                                                                   cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGa                                                         >  1:1033387/1‑134 (MQ=255)
                                                                                   cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCa                                                   >  2:74588/1‑140 (MQ=255)
                                                                                   cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCa                                                   >  2:1337039/1‑140 (MQ=255)
                                                                                      aTCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCAttt                                                >  2:15207/1‑140 (MQ=255)
                                                                                           ctctTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGa                                           <  2:1007649/140‑1 (MQ=255)
                                                                                           ctctTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGa                                           <  1:356185/140‑1 (MQ=255)
                                                                                           ctctTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGa                                           <  2:765279/140‑1 (MQ=255)
                                                                                                 ttcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCa                                     >  1:166382/1‑140 (MQ=255)
                                                                                                    gCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCaaaa                                  <  1:202886/140‑1 (MQ=255)
                                                                                                    gCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCaaaa                                  <  1:279447/140‑1 (MQ=255)
                                                                                                    gCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCaaaa                                  <  1:160930/140‑1 (MQ=255)
                                                                                                     cTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAAt                                 <  2:1139274/140‑1 (MQ=255)
                                                                                                     cTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAAt                                 >  1:864388/1‑140 (MQ=255)
                                                                                                        tagtaCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg                              >  2:225195/1‑140 (MQ=255)
                                                                                                          gtaCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCg                            >  1:1323077/1‑140 (MQ=255)
                                                                                                           taCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg                              <  2:44572/137‑1 (MQ=255)
                                                                                                           taCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg                              >  1:44572/1‑137 (MQ=255)
                                                                                                             cGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGACCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTaa                         >  2:1255942/1‑140 (MQ=255)
                                                                                                                cACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGcc                      >  1:1369217/1‑140 (MQ=255)
                                                                                                                cACCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGcc                      >  1:1009078/1‑140 (MQ=255)
                                                                                                                 aCCAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCGCATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCa                     <  2:533838/140‑1 (MQ=255)
                                                                                                                  ccAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCAc                    <  2:661203/140‑1 (MQ=255)
                                                                                                                  ccAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCAc                    >  2:368102/1‑140 (MQ=255)
                                                                                                                  ccAGGCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCAc                    >  1:1083133/1‑140 (MQ=255)
                                                                                                                     ggCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGt                 >  1:284268/1‑140 (MQ=255)
                                                                                                                     ggCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGt                 >  2:1285616/1‑140 (MQ=255)
                                                                                                                      gCTGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGtt                <  2:864388/140‑1 (MQ=255)
                                                                                                                        tGGTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTc              <  2:1323077/140‑1 (MQ=255)
                                                                                                                          gTCACGGTTTGCAGTGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGc                       <  1:897735/129‑1 (MQ=255)
                                                                                                                          gTCACGGTTTGCAGTGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGc                       >  2:897735/1‑129 (MQ=255)
                                                                                                                          gTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCa            >  1:522372/1‑140 (MQ=255)
                                                                                                                          gTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCa            <  2:284268/140‑1 (MQ=255)
                                                                                                                          gTCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCACTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCa            >  1:845753/1‑140 (MQ=255)
                                                                                                                           tCACGGTTTGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAGTCAGCGTATGCCACGGTTTCCAt           <  2:385598/140‑1 (MQ=255)
                                                                                                                              cGGTTTGCAGCGTTTTTTTGCCCCACATAAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGc        <  1:808290/140‑1 (MQ=255)
                                                                                                                                 tttGCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCACTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTtc     <  2:845753/140‑1 (MQ=255)
                                                                                                                                    gCAGCGTTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTtcctc  >  2:47569/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
TTTCCGCACACAACAGCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCG‑TTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTC  >  NC_000913/3220986‑3221256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑